Top

Published in:

01-04-2006 | Original Article

Low-energy irradiation stimulates formation of osteoclast-like cells via RANK expression in vitro

Authors: Norihito Aihara, Masaru Yamaguchi, Kazutaka Kasai

Published in: Lasers in Medical Science | Issue 1/2006

Abstract

Low-energy laser irradiation (Ga–Al–As semiconductor laser, output 50 mW) was applied to rat osteoclast precursor cells for 1, 3, 6, or 10 min at 24-h intervals during the culture period. The number of tartrate-resistant acid phosphatase positive multinucleate cells was increased by approximately 1.3-fold in the 3- and 6-min irradiation groups. Further, osteoclasts appeared on day 2 in the laser irradiation groups but not until day 3 in the control groups. In immunohistochemical staining for receptor activator of NF-κB (RANK), the laser irradiation groups showed significantly greater amounts of staining in comparison with the control group on days 2 and 3. Reverse transcription-polymerase chain reaction results also showed that the expressions of RANK were upregulated. In the pit formation assay, resorption pits were significantly more abundant in the laser irradiation groups than in the controls. The present results suggest that low-energy laser irradiation facilitates differentiation and activation of osteoclasts via RANK expression.

Kana JS, Hutschenreiter G, Haina D, Waidelich W (1981) Effect of low-power density laser radiation on healing of open skin wounds in rats. Arch Surg 116:293–296PubMed

Mester E, Mester AF, Mester A (1985) The biomedical effects of laser application. Lasers Surg Med 5:31–39PubMedCrossRef

Mester E, Nagylucskay S, Tisza S, Mester A (1978) Stimulation of wound healing by means of laser rays. Part III—investigation of the effect on immune competent cells. Acta Chir Acad Sci Hung 19:163–170PubMed

Oudry M, Franquin JC, Pourreau-Schreider N, Martin PM (1988) Effect of a helium–neon laser on cellular growth: an in vitro study of human gingival fibroblasts. J Biol Buccale 16:129–135

van Breugel HH, Bar PR (1992) Power density and exposure time of He–Ne laser irradiation are more important than total energy dose in photo-biomodulation of human fibroblasts in vitro. Lasers Surg Med 12:528–537PubMedCrossRef

Schultz RJ, Krishnamurthy S, Thelmo W, Rodriguez JE, Harvey G (1985) Effects of varying intensities of laser energy on articular cartilage: a preliminary study. Lasers Surg Med 5:577–588PubMedCrossRef

Abergel RP, Meeker CA, Lam TS, Dwyer RM, Lesavoy MA, Uitto J (1984) Control of connective tissue metabolism by lasers: recent developments and future prospects. J Am Acad Dermatol 11:1142–1150PubMed

Balboni GC, Brandi ML, Zonefrati R, Repice F (1986) Effects of He–Ne/I. R. laser irradiation on two lines of normal human fibroblasts in vitro. Arch Ital Anat Embriol 91:179–188PubMed

Bosatra M, Jucci A, Olliaro P, Quacci D, Sacchi S (1984) In vitro fibroblast and dermis fibroblast activation by laser irradiation at low energy. An electron microscopic study. Dermatologica 168:157–162PubMedCrossRef

10.

Anders JJ, Borke RC, Woolery SK, Van de Merwe WP (1993) Low power laser irradiation alters the rate of regeneration of the rat facial nerve. Lasers Surg Med 13:72–82PubMedCrossRef

11.

Tang XM, Chai BP (1986) Effect of CO2 laser irradiation on experimental fracture healing: a transmission electron microscopic study. Lasers Surg Med 6:346–352PubMedCrossRef

12.

Trelles MA, Mayayo E (1987) Bone fracture consolidates faster with low-power laser. Lasers Surg Med 7:36–45PubMedCrossRef

13.

Lim HM, Lew KK, Tay DK (1995) A clinical investigation of the efficacy of low level laser therapy in reducing orthodontic postadjustment pain. Am J Orthod Dentofac Orthop 108:614–622CrossRef

14.

Rodrigues MTJ, Ribeiro MS, Groth EB, Zezell DM (2002) Evaluation of effects of laser therapy (λ=830 nm) on oral ulceration induced by fixed orthodontic appliances. Lasers Surg Med 30(Suppl 14):15

15.

Saito S, Shimizu N (1997) Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat. Am J Orthod Dentofac Orthop 111:525–532CrossRef

16.

Ozawa Y, Shimizu N, Kariya G, Abiko Y (1998) Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22:347–354PubMedCrossRef

17.

Kawasaki K, Shimizu N (2000) Effects of low-energy laser irradiation on bone remodeling during experimental tooth movement in rats. Lasers Surg Med 26:282–291PubMedCrossRef

18.

Rodan GA (1996) Coupling of bone resorption and formation during bone remodeling. In: Marcus R, Feldman D, Kelsey J (eds) Osteoporosis. Academic, New York, pp 289–299

19.

Lemaire V, Tobin FL, Greller LD, Cho CR, Suva LJ (2004) Modeling the interactions between osteoblast and osteoclast activities in bone remodeling. J Theor Biol 229:293–309PubMedCrossRef

20.

Cruz DR, Kohara EK, Ribeiro MS, Wetter NU (2004) Effects of low-intensity laser therapy on the orthodontic movement velocity of human teeth: a preliminary study. Lasers Surg Med 35:117–120PubMedCrossRef

21.

Roodman GD (1996) Advances in bone biology: the osteoclast. Endocr Rev 17:308–332PubMedCrossRef

22.

Roodman GD, Ibbotson KJ, MacDonald BR, Kuehl TJ, Mundy GR (1985) 1,25-Dihydroxyvitamin D3 causes formation of multinucleated cells with several osteoclast characteristics in cultures of primate marrow. Proc Natl Acad Sci U S A 82:8213–8217PubMedCrossRef

23.

Chambers TJ, Revell PA, Fuller K, Athanasou NA (1984) Resorption of bone by isolated rabbit osteoclasts. J Cell Sci 66:383–399PubMed

24.

Anderson DM, Maraskovsky E, Billingsley WL, Dougall WC, Tometsko ME, Roux ER, Teepe MC, DuBose RF, Cosman D, Galibert L (1997) A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function. Nature 390:175–179PubMedCrossRef

25.

Takeshita S, Kaji K, Kudo A (2000) Identification and characterization of the new osteoclast progenitor with macrophage phenotypes being able to differentiate into mature osteoclasts. J Bone Miner Res 15:1477–1488PubMedCrossRef

26.

Myers DE, Collier FM, Minkin C, Wang H, Holloway WR, Malakellis M, Nicholson GC (1999) Expression of functional RANK on mature rat and human osteoclasts. FEBS Lett 463:295–300PubMedCrossRef

27.

Kanzaki H, Chiba M, Shimizu Y, Mitani H (2002) Priodontal ligament cells under mechanical stress induce osteoclastogenesis by receptor activator of nuclear factor kappaB ligand up-regulation via prostaglandin E2 synthesis. J Bone Miner Res 17:210–220PubMedCrossRef

28.

Suda T, Takahashi N, Udagawa N, Jimi E, Gillespie MT, Martin TJ (1999) Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr Rev 20:345–357PubMedCrossRef

29.

Klebanov GI, Kreinina MV, Poltanov EA, Khristoforova TV, Vladimirov YA (2001) Mechanism of therapeutic effect of low-intensity infrared laser radiation. Bull Exp Biol Med 131:239–241PubMedCrossRef

30.

Wong BR, Rho J, Arron J, Robinson E, Orlinick J, Chao M, Kalachikov S, Cayani E, Bartlett FS 3rd, Frankel WN, Lee SY, Choi Y (1997) TRANCE is a novel ligand of the tumor necrosis factor receptor family that activates c-Jun N-terminal kinase in T cells. J Biol Chem 272:25190–25194PubMedCrossRef

31.

Yasuda H, Shima N, Nakagawa N, Yamaguchi K, Kinosaki M, Mochizuki S, Tomoyasu A, Yano K, Goto M, Murakami A, Tsuda E, Morinaga T, Higashio K, Udagawa N, Takahashi N, Suda T (1998) Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL. Proc Natl Acad Sci U S A 95:3597–3602PubMedCrossRef

32.

Hsu H, Lacey DL, Dunstan CR, Solovyev I, Colombero A, Timms E, Tan HL, Elliott G, Kelley MJ, Sarosi I, Wang L, Xia XZ, Elliott R, Chiu L, Black T, Scully S, Capparelli C, Morony S, Shimamoto G, Bass MB, Boyle WJ (1999) Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand. Proc Natl Acad Sci U S A 96:3540–3545PubMedCrossRef

33.

Udagawa N, Takahashi N, Jimi E, Matsuzaki K, Tsurukai T, Itoh K, Nakagawa N, Yasuda H, Goto M, Tsuda E, Higashio K, Gillespie MT, Martin TJ, Suda T (1999) Osteoblasts/stromal cells stimulate osteoclast activation through expression of osteoclast differentiation factor/RANKL but not macrophage colony-stimulating factor: receptor activator of NF-kappa B ligand. Bone 25:517–523PubMedCrossRef

34.

Ueda Y, Shimizu N (2003) Effects of pulse frequency of low-level laser therapy (LLLT) on bone nodule formation in rat calvarial cells. J Clin Laser Med Surg 21:271–277PubMedCrossRef

35.

Dortbudak O, Haas R, Mailath-Pokorny G (2002) Effect of low-power laser irradiation on bony implant sites. Clin Oral Implants Res 13:288–292PubMedCrossRef

36.

Barushka O, Yaakobi T, Oron U (1995) Effect of low-energy laser (He–Ne) irradiation on the process of bone repair in the rat tibia. Bone 16:47–55PubMedCrossRef

37.

Simonet WS, Lacey DL, Dunstan CR, Kelley M, Chang MS, Luthy R, Nguyen HQ, Wooden S, Bennett L, Boone T, Shimamoto G, DeRose M, Elliott R, Colombero A, Tan HL, Trail G, Sullivan J, Davy E, Bucay N, Renshaw-Gegg L, Hughes TM, Hill D, Pattison W, Campbell P, Sander S, Van G, Tarpley J, Derby P, Lee R, Boyle WJ (1997) Osteoprotegerin: a novel secreted protein involved in the regulation of bone density. Cell 89:309–319PubMedCrossRef

38.

Ogasawara T, Yoshimine Y, Kiyoshima T, Kobayashi I, Matsuo K, Akamine A, Sakai H (2004) In situ expression of RANKL, RANK, osteoprotegerin and cytokines in osteoclasts of rat periodontal tissue. J Periodontal Res 39:42–49PubMedCrossRef

39.

Oshiro T, Shiotani A, Shibasaki Y, Sasaki T (2002) Osteoclast induction in periodontal tissue during experimental movement of incisors in osteoprotegerin-deficient mice. Anat Rec 266:218–225PubMedCrossRef

40.

Kanzaki H, Chiba M, Takahashi I, Haruyama N, Nishimura M, Mitani H (2004) Local OPG gene transfer to periodontal tissue inhibits orthodontic tooth movement. J Dent Res 83:920–925PubMedCrossRef

Title: Low-energy irradiation stimulates formation of osteoclast-like cells via RANK expression in vitro
Authors: Norihito Aihara
Masaru Yamaguchi
Kazutaka Kasai
Publication date: 01-04-2006
Publisher: Springer-Verlag
Published in: Lasers in Medical Science / Issue 1/2006
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-005-0368-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Low-energy irradiation stimulates formation of osteoclast-like cells via RANK expression in vitro

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 1/2006

A comparison study of the efficacy and side effects of different light sources in hair removal

Closure of skin incisions by 980-nm diode laser welding

Action of low-level laser therapy on living fatty tissue of rats

Monitoring the ups and downs of pulsed dye laser energy output

Pain reduction by infrared light-emitting diode irradiation: a pilot study on experimentally induced delayed-onset muscle soreness in humans

A new user-friendly software platform for systematic classification of skin lesions to aid in their diagnosis and prognosis