Top

Published in:

01-01-2015 | Original Article

Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors

Authors: Mishie Tanino, Toshio Sasajima, Hiroshi Nanjo, Shiori Akesaka, Masami Kagaya, Taichi Kimura, Yusuke Ishida, Masaya Oda, Masataka Takahashi, Taku Sugawara, Toshiaki Yoshioka, Hiroshi Nishihara, Yoichi Akagami, Akiteru Goto, Yoshihiro Minamiya, Shinya Tanaka, R-IHC Study Group

Published in: Brain Tumor Pathology | Issue 1/2015

Abstract

Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen–antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 90.7 % (168/183 cases) in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma. To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors.

Available only for authorised users

Brat DJ, Prayson RA, Ryken TC et al (2008) Diagnosis of malignant glioma: role of neuropathology. J Neurooncol 89(3):287–311PubMedCrossRef

Faehndrich J, Weidauer S, Pilatus U et al (2011) Neuroradiological viewpoint on the diagnostics of space-occupying brain lesions. Clin Neuroradiol 21(3):123–139PubMedCrossRef

Nihashi T, Dahabreh IJ, Terasawa T (2013) Diagnostic accuracy of PET for recurrent glioma diagnosis: a meta-analysis. AJNR 34(5):944–950 S1–S11PubMedCrossRef

Louis DN, Ohgaki H, Wiestler OD et al (2007) WHO classification of tumors of the central nervous system. IARC, Lyon

Plesec TP, Prayson RA (2007) Frozen section discrepancy in the evaluation of central nervous system tumors. Arch Pathol Lab Med 131(10):1532–1540PubMed

Uematsu Y, Owai Y, Okita R et al (2007) The usefulness and problem of intraoperative rapid diagnosis in surgical neuropathology. Brain Tumor Pathol 24(2):47–52PubMedCrossRef

Ichihara T, Nakao A, Suzuki Y et al (1989) Improvement of the rapid immunoperoxidase staining method for intraoperative pathological diagnosis of pancreatic cancer using microwave irradiation. J Surg Oncol 42(3):209–214PubMedCrossRef

Richter T, Nahrig J, Komminoth P et al (1999) Protocol for ultrarapid immunostaining of frozen sections. J Clin Pathol 52(6):461–463PubMedCentralPubMedCrossRef

Kammerer U, Kapp M, Gassel AM et al (2001) A new rapid immunohistochemical staining technique using the EnVision antibody complex. J Histochem Cytochem 49(5):623–630PubMedCrossRef

10.

Haapasalo J, Mennander A, Helen P et al (2005) Ultrarapid Ki-67 immunostaining in frozen section interpretation of gliomas. J Clin Pathol 58(3):263–268PubMedCentralPubMedCrossRef

11.

Uzuka T, Aoki H, Natsumeda M et al (2011) Indication of intraoperative immunohistochemistry for accurate pathological diagnosis of brain tumors. Brain Tumor Pathol 28(3):239–246PubMedCrossRef

12.

Monig SP, Luebke T, Soheili A et al (2006) Rapid immunohistochemical detection of tumor cells in gastric carcinoma. Oncol Rep 16(5):1143–1147PubMed

13.

Hatta H, Tsuneyama K, Kumada T et al (2006) Freshly prepared immune complexes with intermittent microwave irradiation result in rapid and high-quality immunostaining. Pathol Res Pract 202(6):439–445PubMedCrossRef

14.

Hatta H, Tsuneyama K, Kondo T et al (2010) Development of an ultrasound-emitting device for performing rapid immunostaining procedures. J Histochem Cytochem 58(5):421–428PubMedCentralPubMedCrossRef

15.

Toda H, Minamiya Y, Kagaya M et al (2011) A novel immunohistochemical staining method allows ultrarapid detection of lymph node micrometastases while conserving antibody. Acta Histochem Cytochem 44(3):133–139PubMedCentralPubMedCrossRef

16.

Firlik KS, Martinez AJ, Lunsford LD (1999) Use of cytological preparations for the intraoperative diagnosis of stereotactically obtained brain biopsies: a 19-year experience and survey of neuropathologists. J Neurosurg 91(3):454–458PubMedCrossRef

Title: Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors
Authors: Mishie Tanino
Toshio Sasajima
Hiroshi Nanjo
Shiori Akesaka
Masami Kagaya
Taichi Kimura
Yusuke Ishida
Masaya Oda
Masataka Takahashi
Taku Sugawara
Toshiaki Yoshioka
Hiroshi Nishihara
Yoichi Akagami
Akiteru Goto
Yoshihiro Minamiya
Shinya Tanaka
R-IHC Study Group
Publication date: 01-01-2015
Publisher: Springer Japan
Published in: Brain Tumor Pathology / Issue 1/2015
Print ISSN: 1433-7398
Electronic ISSN: 1861-387X
DOI: https://doi.org/10.1007/s10014-014-0188-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 1/2015

The perivascular microenvironment in primary central nervous system lymphomas: the role of chemokines and the endothelin B receptor

Preface to special issue

Primary diffuse leptomeningeal glioneuronal tumors

Erratum to: Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors

Isolated spinal Rosai–Dorfman disease misdiagnosed as lymphoplasmacyte-rich meningioma by intraoperative histological examination

Third ventricular atypical meningioma which recurred with further malignant progression