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Medical Molecular Morphology
Published in: Medical Molecular Morphology 1/2013

01-03-2013 | Original Paper

Localization of multidomain adaptor proteins, p140Cap and vinexin, in the pancreatic islet of a spontaneous diabetes mellitus model, Otsuka Long-Evans Tokushima Fatty rats

Authors: Masahiro Yamauchi, Kaori Sudo, Hidenori Ito, Ikuko Iwamoto, Rika Morishita, Toshihiro Murai, Kazuo Kajita, Tatsuo Ishizuka, Koh-ichi Nagata

Published in: Medical Molecular Morphology | Issue 1/2013

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Abstract

We have shown that two multidomain adaptor proteins, p140Cap and vinexin, interact with each other and are likely to be involved in neurotransmitter release. Because the basic molecular mechanism governing neurotransmitter and insulin secretion is conserved, these two proteins may also to play pivotal roles in insulin secretion. We therefore performed some characterization of p140Cap and vinexin in pancreas of a wild-type rat or a spontaneous type 2 diabetes mellitus (DM) model, the Otsuka Long-Evans Tokushima Fatty (OLETF) rat. These two proteins were detected in Wistar rat pancreas by Western blotting. Immunohistochemistry revealed that p140Cap and vinexin are enriched in β and α cells, respectively, in the rat pancreas. We then found that pancreatic islet structure was disorganized in the OLETF rat with hyperinsulinemia or with hyperglycemia, based on immunohistochemical analyses of vinexin. In β cells of these model rats, p140Cap was distributed in a cytoplasmic granular pattern as in the control rats, although its expression was reduced to various extents from cell to cell. These results may suggest possible involvement of p140Cap in insulin secretion, and reduction of p140Cap might be related to abnormal insulin secretion in DM.
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Metadata
Title
Localization of multidomain adaptor proteins, p140Cap and vinexin, in the pancreatic islet of a spontaneous diabetes mellitus model, Otsuka Long-Evans Tokushima Fatty rats
Authors
Masahiro Yamauchi
Kaori Sudo
Hidenori Ito
Ikuko Iwamoto
Rika Morishita
Toshihiro Murai
Kazuo Kajita
Tatsuo Ishizuka
Koh-ichi Nagata
Publication date
01-03-2013
Publisher
Springer Japan
Published in
Medical Molecular Morphology / Issue 1/2013
Print ISSN: 1860-1480
Electronic ISSN: 1860-1499
DOI
https://doi.org/10.1007/s00795-013-0008-1

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