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01-10-2020 | Root Canal Debridement | Original Article

Clinical endodontic procedures modulate periapical cytokine and chemokine gene expressions

Authors: Lucas Moreira Maia, Marcela Carvalho Espaladori, Julia Maria Braga Diniz, Warley Luciano Faria Tavares, Luciana Carla Neves de Brito, Leda Quércia Vieira, Antônio Paulino Ribeiro Sobrinho

Published in: Clinical Oral Investigations | Issue 10/2020

Abstract

Objectives

To identify the gene expression of the cytokines IL-9, TNF-α, IL-1, INF-γ, IL-17A, and IL-10 and the chemokines CCL-2/MCP-1 and CCR-6 in the periapical fluid of human root canal infections.

Materials and methods

Twenty samples were collected immediately and 7 days after the cleaning and shaping procedures (after reducing the intracanal microbial load) in an attempt to characterize the expression of these genes. The endogenous expression levels of cytokines and chemokines were analyzed by real-time polymerase chain reaction. The Shapiro-Wilk and the Wilcoxon tests analyzed data.

Results

Significantly higher levels of the IL-9, INF-γ, TNF-α, IL-1, and IL-10 markers on day 7 were observed compared with day 0 (p < 0.05). However, IL-17A and the chemokines CCL-2/MCP-1 and CCR-6 did not show a significant difference in mRNA expression when comparing both timepoints (p > 0.05).

Conclusions

The clinical variation of the periapical immune status after endodontic therapy suggests that the cytokine and chemokine-mediated pro-inflammatory response appears to be modulated in an IL-10/IL-9-dependent manner.

Clinical relevance

Few studies have investigated the role of Th9 cells in periapical lesions. IL-9 presents exciting plasticity, performing immunosuppressive actions, and it is also capable of changing their phenotype in the presence of IL-17. Hence, it is relevant to investigate its role in the context of the known mediators involved the periapical immune process.

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Title: Clinical endodontic procedures modulate periapical cytokine and chemokine gene expressions
Authors: Lucas Moreira Maia
Marcela Carvalho Espaladori
Julia Maria Braga Diniz
Warley Luciano Faria Tavares
Luciana Carla Neves de Brito
Leda Quércia Vieira
Antônio Paulino Ribeiro Sobrinho
Publication date: 01-10-2020
Publisher: Springer Berlin Heidelberg
Keywords: Root Canal Debridement
Root Canal Debridement
Cytokines
Published in: Clinical Oral Investigations / Issue 10/2020
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-020-03247-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Clinical endodontic procedures modulate periapical cytokine and chemokine gene expressions

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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