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Clinical Oral Investigations
Published in: Clinical Oral Investigations 2/2020

01-02-2020 | Original Article

Synergistic potential of 1α,25-dihydroxyvitamin D3 and calcium–aluminate–chitosan scaffolds with dental pulp cells

Authors: Ester Alves Ferreira Bordini, Fernanda Balestrero Cassiano, Isabela Sanches Pompeo Silva, Felipe Rochelle Usberti, Giovana Anovazzi, Leandro Edgar Pacheco, Taísa Nogueira Pansani, Maria Luísa Leite, Josimeri Hebling, Carlos Alberto de Souza Costa, Diana Gabriela Soares

Published in: Clinical Oral Investigations | Issue 2/2020

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Abstract

Objectives

This study aimed to develop a porous chitosan–calcium–aluminate scaffold (CH-AlCa) in combination with a bioactive dosage of 1α,25-dihydroxyvitamin D3 (1α,25VD), to be used as a bioactive substrate capable to increase the odontogenic potential of human dental pulp cells (HDPCs).

Materials and methods

The porous CH-AlCa was developed by the incorporation of an AlCa suspension into a CH solution under vigorous agitation, followed by phase separation at low temperature. Scaffold architecture, porosity, and calcium release were evaluated. Thereafter, the synergistic potential of CH-AlCa and 1 nM 1α,25VD, selected by a dose–response assay, for HDPCs seeded onto the materials was assessed.

Results

The CH-AlCa featured an organized and interconnected pore network, with increased porosity in comparison with that of plain chitosan scaffolds (CH). Increased odontoblastic phenotype expression on the human dental pulp cell (HDPC)/CH and HDPC/CH-AlCa constructs in the presence of 1 nM 1α,25VD was detected, since alkaline phosphatase activity, mineralized matrix deposition, dentin sialophosphoprotein/dentin matrix acidic phosphoprotein 1 mRNA expression, and cell migration were overstimulated. This drug featured a synergistic effect with CH-AlCa, since the highest values of cell migration and odontoblastic markers expression were observed in this experimental condition.

Conclusions

The experimental CH-AlCa scaffold increases the chemotaxis and regenerative potential of HDPCs, and the addition of low-dosage 1α,25VD to this scaffold enhances the potential of these cells to express an odontoblastic phenotype.

Clinical relevance

Chitosan scaffolds enriched with calcium–aluminate in association with low dosages of 1α,25-dihydroxyvitamin D3 provide a highly bioactive microenvironment for dental pulp cells prone to dentin regeneration, thus providing potential as a cell-free tissue engineering system for direct pulp capping.
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Metadata
Title
Synergistic potential of 1α,25-dihydroxyvitamin D3 and calcium–aluminate–chitosan scaffolds with dental pulp cells
Authors
Ester Alves Ferreira Bordini
Fernanda Balestrero Cassiano
Isabela Sanches Pompeo Silva
Felipe Rochelle Usberti
Giovana Anovazzi
Leandro Edgar Pacheco
Taísa Nogueira Pansani
Maria Luísa Leite
Josimeri Hebling
Carlos Alberto de Souza Costa
Diana Gabriela Soares
Publication date
01-02-2020
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 2/2020
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-019-02906-z

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