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01-12-2016 | Original Article

Comparative release of growth factors from PRP, PRF, and advanced-PRF

Authors: Eizaburo Kobayashi, Laura Flückiger, Masako Fujioka-Kobayashi, Kosaku Sawada, Anton Sculean, Benoit Schaller, Richard J. Miron

Published in: Clinical Oral Investigations | Issue 9/2016

Abstract

Objectives

The use of platelet concentrates has gained increasing awareness in recent years for regenerative procedures in modern dentistry. The aim of the present study was to compare growth factor release over time from platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and a modernized protocol for PRF, advanced-PRF (A-PRF).

Materials and methods

Eighteen blood samples were collected from six donors (3 samples each for PRP, PRF, and A-PRF). Following preparation, samples were incubated in a plate shaker and assessed for growth factor release at 15 min, 60 min, 8 h, 1 day, 3 days, and 10 days. Thereafter, growth factor release of PDGF-AA, PDGF-AB, PDGF-BB, TGFB1, VEGF, EGF, and IGF was quantified using ELISA.

Results

The highest reported growth factor released from platelet concentrates was PDGF-AA followed by PDGF-BB, TGFB1, VEGF, and PDGF-AB. In general, following 15–60 min incubation, PRP released significantly higher growth factors when compared to PRF and A-PRF. At later time points up to 10 days, it was routinely found that A-PRF released the highest total growth factors. Furthermore, A-PRF released significantly higher total protein accumulated over a 10-day period when compared to PRP or PRF.

Conclusion

The results from the present study indicate that the various platelet concentrates have quite different release kinetics. The advantage of PRP is the release of significantly higher proteins at earlier time points whereas PRF displayed a continual and steady release of growth factors over a 10-day period. Furthermore, in general, it was observed that the new formulation of PRF (A-PRF) released significantly higher total quantities of growth factors when compared to traditional PRF.

Clinical relevance

Based on these findings, PRP can be recommended for fast delivery of growth factors whereas A-PRF is better-suited for long-term release.

Dohan Ehrenfest DM, Wang HL, Galindo-Moreno P, Bowler D, Dym H (2015) Bone morphogenic protein: application in implant dentistry. BioMed Res Int 59:493–503. doi:10.1155/2015/34132710.1016/j.cden.2014.10.006

Padial-Molina M, O’Valle F, Lanis A and Mesa F (2015) Clinical application of mesenchymal stem cells and novel supportive therapies for oral bone regeneration. Biomed Res Int 2015:341327. doi:10.1155/2015/341327

Sanz-Sanchez I, Ortiz-Vigon A, Sanz-Martin I, Figuero E, Sanz M (2015) Effectiveness of lateral bone augmentation on the alveolar crest dimension: a systematic review and meta-analysis. J Dent Res 94:128s–142s. doi:10.1177/0022034515594780 CrossRefPubMed

Miron RJ, Zhang YF (2012) Osteoinduction: a review of old concepts with new standards. J Dent Res 91:736–744. doi:10.1177/0022034511435260 CrossRefPubMed

Anusaksathien O, Giannobile WV (2002) Growth factor delivery to re-engineer periodontal tissues. Curr Pharm Biotechnol 3:129–139CrossRefPubMed

Carreira AC, Lojudice FH, Halcsik E, Navarro RD, Sogayar MC, Granjeiro JM (2014) Bone morphogenetic proteins: facts, challenges, and future perspectives. J Dent Res 93:335–345. doi:10.1177/0022034513518561 CrossRefPubMed

Rocque BG, Kelly MP, Miller JH, Li Y, Anderson PA (2014) Bone morphogenetic protein-associated complications in pediatric spinal fusion in the early postoperative period: an analysis of 4658 patients and review of the literature. J Neurosurg Pediatr 14:635–643. doi:10.3171/2014.8.peds13665 CrossRefPubMed

Kaigler D, Avila G, Wisner-Lynch L, Nevins ML, Nevins M, Rasperini G, Lynch SE, Giannobile WV (2011) Platelet-derived growth factor applications in periodontal and peri-implant bone regeneration. Expert Opin Biol Ther 11:375–385. doi:10.1517/14712598.2011.554814 CrossRefPubMedPubMedCentral

Del Corso M, Vervelle A, Simonpieri A, Jimbo R, Inchingolo F, Sammartino G, Dohan Ehrenfest DM (2012) Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 1: periodontal and dentoalveolar surgery. Curr Pharm Biotechnol 13:1207–1230CrossRefPubMed

10.

Simonpieri A, Del Corso M, Vervelle A, Jimbo R, Inchingolo F, Sammartino G, Dohan Ehrenfest DM (2012) Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 2: bone graft, implant and reconstructive surgery. Curr Pharm Biotechnol 13:1231–1256CrossRefPubMed

11.

Medina-Porqueres I, Alvarez-Juarez P (2015) The efficacy of platelet-rich plasma injection in the management of hip osteoarthritis: a systematic review protocol. Musculoskeletal Care. doi:10.1002/msc.1115 PubMed

12.

Salamanna F, Veronesi F, Maglio M and Della Bella E (2015) New and emerging strategies in platelet-rich plasma application in musculoskeletal regenerative procedures: general overview on still open questions and outlook. 2015:846045. doi: 10.1155/2015/846045

13.

Albanese A, Licata ME, Polizzi B, Campisi G (2013) Platelet-rich plasma (PRP) in dental and oral surgery: from the wound healing to bone regeneration. Immun Ageing 10:23. doi:10.1186/1742-4933-10-23 CrossRefPubMedPubMedCentral

14.

Dohan Ehrenfest DM, Andia I, Zumstein MA, Zhang CQ, Pinto NR, Bielecki T (2014) Classification of platelet concentrates (platelet-rich plasma-PRP, platelet-rich fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectives. Muscles, Ligament Tendons J 4:3–9

15.

Maney P, Amornporncharoen M, Palaiologou A (2013) Applications of plasma rich in growth factors (PRGF) in dental surgery: a review. J West Soc of Periodontol Periodontal Abstr 61:99–104

16.

Panda S, Doraiswamy J, Malaiappan S, Varghese SS and Del Fabbro M (2014) Additive effect of autologous platelet concentrates in treatment of intrabony defects: a systematic review and meta-analysis. J Investig Clin Dent. doi: 10.1111/jicd.12117

17.

Marx RE (2001) Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dent 10:225–228CrossRefPubMed

18.

Marx RE (2004) Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg Off J Am Assoc Oral Maxillofac Surg 62:489–496CrossRef

19.

Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998) Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:638–646CrossRefPubMed

20.

Kukreja BJ, Dodwad V, Kukreja P, Ahuja S, Mehra P (2014) A comparative evaluation of platelet-rich plasma in combination with demineralized freeze-dried bone allograft and DFDBA alone in the treatment of periodontal intrabony defects: a clinicoradiographic study. J Indian Soc of Periodontol 18:618–623. doi:10.4103/0972-124x.142457 CrossRef

21.

Pradeep AR, Rao NS, Agarwal E, Bajaj P, Kumari M, Naik SB (2012) Comparative evaluation of autologous platelet-rich fibrin and platelet-rich plasma in the treatment of 3-wall intrabony defects in chronic periodontitis: a randomized controlled clinical trial. J Periodontol 83:1499–1507. doi:10.1902/jop.2012.110705 CrossRefPubMed

22.

Dori F, Arweiler N, Huszar T, Gera I, Miron RJ, Sculean A (2013) Five-year results evaluating the effects of platelet-rich plasma on the healing of intrabony defects treated with enamel matrix derivative and natural bone mineral. J Periodontol 84:1546–1555. doi:10.1902/jop.2013.120501 CrossRefPubMed

23.

Ozdemir B, Okte E (2012) Treatment of intrabony defects with beta-tricalciumphosphate alone and in combination with platelet-rich plasma. J Biomed Mater Res B, Appl Biomater 100:976–983. doi:10.1002/jbm.b.32660 CrossRef

24.

Yilmaz S, Kabadayi C, Ipci SD, Cakar G, Kuru B (2011) Treatment of intrabony periodontal defects with platelet-rich plasma versus platelet-poor plasma combined with a bovine-derived xenograft: a controlled clinical trial. J Periodontol 82:837–844. doi:10.1902/jop.2010.100503 CrossRefPubMed

25.

Camargo PM, Lekovic V, Weinlaender M, Divnic-Resnik T, Pavlovic M, Kenney EB (2009) A surgical reentry study on the influence of platelet-rich plasma in enhancing the regenerative effects of bovine porous bone mineral and guided tissue regeneration in the treatment of intrabony defects in humans. J Periodontol 80:915–923. doi:10.1902/jop.2009.080600 CrossRefPubMed

26.

Camargo PM, Lekovic V, Weinlaender M, Vasilic N, Madzarevic M, Kenney EB (2005) A reentry study on the use of bovine porous bone mineral, GTR, and platelet-rich plasma in the regenerative treatment of intrabony defects in humans. Int J Periodontics Restorative Dent 25:49–59PubMed

27.

Yassibag-Berkman Z, Tuncer O, Subasioglu T, Kantarci A (2007) Combined use of platelet-rich plasma and bone grafting with or without guided tissue regeneration in the treatment of anterior interproximal defects. J Periodontol 78:801–809. doi:10.1902/jop.2007.060318 CrossRefPubMed

28.

Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, Dohan AJ, Mouhyi J, Dohan DM (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:299–303. doi:10.1016/j.tripleo.2005.07.012 CrossRefPubMed

29.

Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, Gogly B (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e45–e50. doi:10.1016/j.tripleo.2005.07.009 CrossRefPubMed

30.

Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, Gogly B (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e37–e44. doi:10.1016/j.tripleo.2005.07.008 CrossRefPubMed

31.

Sunitha Raja V, Munirathnam Naidu E (2008) Platelet-rich fibrin: evolution of a second-generation platelet concentrate. Indian J Dent Res Off Publ Indian Soc Dental Res 19:42–46CrossRef

32.

Man D, Plosker H, Winland-Brown JE (2001) The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg 107:229–237 discussion 238-9CrossRefPubMed

33.

Lekovic V, Milinkovic I, Aleksic Z, Jankovic S, Stankovic P, Kenney EB, Camargo PM (2012) Platelet-rich fibrin and bovine porous bone mineral vs. platelet-rich fibrin in the treatment of intrabony periodontal defects. J Periodontal Res 47:409–417. doi:10.1111/j.1600-0765.2011.01446.x CrossRefPubMed

34.

Panda S, Jayakumar ND, Sankari M, Varghese SS, Kumar DS (2014) Platelet rich fibrin and xenograft in treatment of intrabony defect. Contemporary Clinical Dentistry 5:550–554. doi:10.4103/0976-237x.142830 CrossRefPubMedPubMedCentral

35.

Sharma A, Pradeep AR (2011) Treatment of 3-wall intrabony defects in patients with chronic periodontitis with autologous platelet-rich fibrin: a randomized controlled clinical trial. J Periodontol 82:1705–1712. doi:10.1902/jop.2011.110075 CrossRefPubMed

36.

Kumar RV, Shubhashini N (2013) Platelet rich fibrin: a new paradigm in periodontal regeneration. Cell Tissue Bank 14:453–463. doi:10.1007/s10561-012-9349-6 CrossRefPubMed

37.

Ghanaati S, Booms P, Orlowska A, Kubesch A, Lorenz J, Rutkowski J, Landes C, Sader R, Kirkpatrick C, Choukroun J (2014) Advanced platelet-rich fibrin: a new concept for cell-based tissue engineering by means of inflammatory cells. J Oral Implantol 40:679–689. doi:10.1563/aaid-joi-D-14-00138 CrossRefPubMed

38.

Gassling VL, Acil Y, Springer IN, Hubert N, Wiltfang J (2009) Platelet-rich plasma and platelet-rich fibrin in human cell culture. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:48–55. doi:10.1016/j.tripleo.2009.02.007 CrossRefPubMed

39.

Miron RJ, Gruber R, Hedbom E, Saulacic N, Zhang Y, Sculean A, Bosshardt DD, Buser D (2013) Impact of bone harvesting techniques on cell viability and the release of growth factors of autografts. Clin Implant Dent Relat Res 15:481–489. doi:10.1111/j.1708-8208.2012.00440.x CrossRefPubMed

40.

El-Sharkawy H, Kantarci A, Deady J, Hasturk H, Liu H, Alshahat M, Van Dyke TE (2007) Platelet-rich plasma: growth factors and pro- and anti-inflammatory properties. J Periodontol 78:661–669. doi:10.1902/jop.2007.060302 CrossRefPubMed

41.

Su CY, Kuo YP, Tseng YH, Su CH, Burnouf T (2009) In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:56–61. doi:10.1016/j.tripleo.2009.02.004 CrossRefPubMed

42.

Okuda K, Kawase T, Momose M, Murata M, Saito Y, Suzuki H, Wolff LF, Yoshie H (2003) Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-beta and modulates the proliferation of periodontally related cells in vitro. J Periodontol 74:849–857. doi:10.1902/jop.2003.74.6.849 CrossRefPubMed

43.

Kawase T, Okuda K, Wolff LF, Yoshie H (2003) Platelet-rich plasma-derived fibrin clot formation stimulates collagen synthesis in periodontal ligament and osteoblastic cells in vitro. J Periodontol 74:858–864. doi:10.1902/jop.2003.74.6.858 CrossRefPubMed

44.

Fan WJ, Yang M, Zhang C, Xue R, Zhang W, Qin HX (2013) Effects of Choukroun’s platelet-rich fibrin on human gingival fibroblasts proliferation, migration and type I collagen secretion. Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi =. Chin J Stomatol 48:72–76

45.

Dohan Ehrenfest DM, Doglioli P, de Peppo GM, Del Corso M, Charrier JB (2010) Choukroun’s platelet-rich fibrin (PRF) stimulates in vitro proliferation and differentiation of human oral bone mesenchymal stem cell in a dose-dependent way. Arch Oral Biol 55:185–194. doi:10.1016/j.archoralbio.2010.01.004 CrossRefPubMed

46.

Kawase T, Okuda K, Saito Y, Amizuka N, Suzuki H, Yoshie H (2005) Platelet-rich plasma provides nucleus for mineralization in cultures of partially differentiated periodontal ligament cells. In Vitro Cell Dev Biol Anim 41:171–176. doi:10.1290/0502013.1 CrossRefPubMed

47.

Kawase T, Tanaka T, Okuda K, Tsuchimochi M, Oda M, Hara T (2015) Quantitative single-cell motility analysis of platelet-rich plasma-treated endothelial cells in vitro. Cytoskeleton (Hoboken, NJ) 72:246–255. doi:10.1002/cm.21221 CrossRef

48.

Gassling V, Hedderich J, Acil Y, Purcz N, Wiltfang J, Douglas T (2013) Comparison of platelet rich fibrin and collagen as osteoblast-seeded scaffolds for bone tissue engineering applications. Clin Oral Implants Res 24:320–328. doi:10.1111/j.1600-0501.2011.02333.x CrossRefPubMed

Title: Comparative release of growth factors from PRP, PRF, and advanced-PRF
Authors: Eizaburo Kobayashi
Laura Flückiger
Masako Fujioka-Kobayashi
Kosaku Sawada
Anton Sculean
Benoit Schaller
Richard J. Miron
Publication date: 01-12-2016
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 9/2016
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-016-1719-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Comparative release of growth factors from PRP, PRF, and advanced-PRF

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

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