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Clinical Oral Investigations

Published in:

01-03-2014 | Original Article

Effects of enamel matrix proteins in combination with a bovine-derived natural bone mineral for the repair of bone defects

Authors: Richard J. Miron, Lingfei Wei, Dieter D. Bosshardt, Daniel Buser, Anton Sculean, Yufeng Zhang

Published in: Clinical Oral Investigations | Issue 2/2014

Abstract

Objectives

Previously, the use of enamel matrix derivative (EMD) in combination with a natural bone mineral (NBM) was able to stimulate periodontal ligament cell and osteoblast proliferation and differentiation. Despite widespread use of EMD for periodontal applications, the effects of EMD on bone regeneration are not well understood. The aim of the present study was to test the ability of EMD on bone regeneration in a rat femur defect model in combination with NBM.

Materials and methods

Twenty-seven rats were treated with either NBM or NBM + EMD and assigned to histological analysis at 2, 4, and 8 weeks. Defect morphology and mineralized bone were assessed by μCT. For descriptive histology, hematoxylin and eosin staining and Safranin O staining were performed.

Results

Significantly more newly formed trabecular bone was observed at 4 weeks around the NBM particles precoated with EMD when compared with NBM particles alone. The drilled control group, in contrast, achieved minimal bone regeneration at all three time points (P < 0.05).

Conclusions

The present results may suggest that EMD has the ability to enhance the speed of new bone formation when combined with NBM particles in rat osseous defects.

Clinical relevance

These findings may provide additional clinical support for the combination of EMD with bone graft for the repair of osseous and periodontal intrabony defects.

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Title: Effects of enamel matrix proteins in combination with a bovine-derived natural bone mineral for the repair of bone defects
Authors: Richard J. Miron
Lingfei Wei
Dieter D. Bosshardt
Daniel Buser
Anton Sculean
Yufeng Zhang
Publication date: 01-03-2014
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 2/2014
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-013-0992-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effects of enamel matrix proteins in combination with a bovine-derived natural bone mineral for the repair of bone defects

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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