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Clinical Oral Investigations

Published in:

01-03-2013 | Original Article

Salivary level of interleukin-8 in oral precancer and oral squamous cell carcinoma

Authors: Silky Rajesh Punyani, Ramhari Shankarrao Sathawane

Published in: Clinical Oral Investigations | Issue 2/2013

Abstract

Background

Interleukin 8 (IL-8) is a pro-angiogenic, pro-inflammatory mediator that belongs to the family of chemokines. Due to its pro-angiogenic characteristic, it may play a vital role in tumour angiogenesis and progression.

Objectives

This study was designed to estimate the levels of salivary IL-8 in oral precancer and oral squamous cell carcinoma (OSCC) patients and compare them with healthy controls. The aim was to evaluate its efficacy as a potential biomarker for these diseases.

Materials and methods

Each group comprised 25 individuals. The salivary IL-8 levels were determined by enzyme-linked immunosorbent assay.

Results

The levels of salivary IL-8 were found to be significantly elevated in patients with OSCC as compared to the precancer group (p < 0.0001) and healthy controls (p < 0.0001). However, the difference in salivary IL-8 concentrations among the precancer group and controls was statistically non-significant (p = 0.738).

Conclusions

Our results suggested that salivary IL-8 can be utilised as a potential biomarker for OSCC. Salivary IL-8 was found to be non-conclusive for oral premalignancy in this preliminary study. Hence, its possible role in transition from premalignancy to malignancy needs further research with larger sample sizes.

Clinical relevance

Saliva as a diagnostic biofluid offers a number of advantages over blood-based testing. The role of IL-8 in oral cancer if validated further by future research can provide an easy diagnostic test as well as a prognostic indicator for patients undergoing treatment. Therefore, if it’s role in tumourigenesis can be sufficiently assessed, it could open up new avenues to find out novel treatment modalities for oral cancer.

Mehrotra R, Yadav S (2006) Oral squamous cell carcinoma: etiology, pathogenesis and prognostic value of genomic alterations. Indian J Cancer 43:60–66PubMedCrossRef

Khanna SS, Karjodkar FR (2006) Circulating immune complexes and trace elements (copper, iron and selenium) as markers in oral precancer and cancer: a randomised, controlled clinical trial. Head Face Med 2:33PubMedCrossRef

Shafer WG, Levy BM, Hine MK, Rajendran R, Sivapathasundharam B (2006) Shafer’s textbook of oral pathology. Elsevier, New Delhi

Oliveira LR, Ribeiro-Silva A (2011) Prognostic significance of immunohistochemical biomarkers in oral squamous cell carcinoma. Int J Oral Maxillofac Surg 40:298–307PubMedCrossRef

Chang YC, Nieh S, Chen S-F, Jao S-W, Lin Y-L, Fu E (2010) Invasive pattern grading score designed as an independent prognostic indicator in oral squamous cell carcinoma. Histopathology 57:295–303PubMedCrossRef

Nagler RM, Barak M, Ben-Aryeh H, Peled M, Filatov M, Laufer D (1999) Early diagnostic and treatment monitoring role of Cyfra 21-1 and TPS in oral squamous cell carcinoma. Cancer 35:1018–1025CrossRef

Bhatavdekar JM, Patel DD, Vora HH, Balar DB (1993) Circulating markers and growth factors as prognosticators in men with advanced tongue cancer. Tumour Biol 14:55–58PubMedCrossRef

Bhatavdekar JM, Patel DD, Vora HH, Balar DB (1993) Circulating prolactin and TPS in monitoring the clinical course of male patients with metastatic tongue cancer: a preliminary study. Anticancer Res 13:237–240PubMed

Krimmel M, Hoffmann J, Krimmel C, Cornelius CP, Schwenzer N (1998) Relevance of SCC-Ag, CEA, CA 19.9, and CA125 for diagnosis and follow-up in oral cancer. J Craniomaxillofac Surg 26:243–248PubMedCrossRef

10.

Kuo WR, Lee KW, Ho KY, Tsai SM, Chiang FY, Juan KH (1999) Tissue polypeptide antigen, carcinoembryonic antigen, carbohydrate antigen, and CA125 levels as tumor markers in squamous cell carcinoma of the head and neck. Kaohsiung J Med Sci 15:152–158PubMed

11.

Hoffmann J, Munz A, Krimmel M, Alfter G (1998) Intraoperative and postoperative kinetics of serum tumor markers in patients with oral carcinoma. J Oral Maxillofac Surg 56:1390–1393PubMedCrossRef

12.

Groschl M, Kohler H, Topf HG, Rupprecht T, Rauh M (2008) Evaluation of saliva collection devices for the analysis of steroids, peptides and therapeutic drugs. J Pharm Biomed Anal 47:478–486PubMedCrossRef

13.

Zimmermann BG, Wong DT (2008) Salivary mRNA targets for cancer diagnostics. Oral Oncol 44:425–429PubMedCrossRef

14.

Young MRI, Wright MA, Lozano Y, Prechel MM, Benefield J, Leonetti JP, Collins SL et al (1997) Increased recurrence and metastasis in patients whose primary head and neck squamous cell carcinomas secreted granulocyte–macrophage colony-stimulating factor and contained CD34⁺ natural suppressor cells. Int J Cancer 74:69–74PubMedCrossRef

15.

Gleich LL, Biddinger PW, Duperier FD, Gluckman JL (1997) Tumor angiogenesis as a prognostic indicator in T2–T4 oral cavity squamous cell carcinoma: a clinical–pathologic correlation. Head Neck 19:276–280PubMedCrossRef

16.

Pak AS, Wright MA, Matthews JP, Collins SL, Petruzzelli GJ, Young MR (1995) Mechanisms of immune suppression in patients with head and neck cancer: presence of CD34(+) cells which suppress immune functions within cancers that secrete granulocyte–macrophage colony-stimulating factor. Clin Cancer Res 1:95–103PubMed

17.

Smith CW, Chen Z, Dong G, Loukinova E, Pegram MY, Figueroa LN, Waes CV (1998) The host environment promotes the development of primary and metastatic squamous cell carcinomas that constitutively express proinflammatory cytokines IL-1 α, IL-6, GM-CSF, and KC. Clin Exp Metastasis 16:655–664PubMedCrossRef

18.

Rhodus NL, Ho V, Miller CS, Myers S, Ondrey F (2005) NF-kappa B dependent cytokine levels in saliva of patients with oral preneoplastic lesions and oral squamous cell carcinoma. Cancer Detect Prev 29:42–45PubMedCrossRef

19.

Liao PH, Chang YC, Huang MF, Tai KW, Chou MY (2000) Mutation of p53 gene codon 63 in saliva as a molecular marker for oral squamous cell carcinomas. Oral Oncol 36:272–276PubMedCrossRef

20.

Zhong LP, Chen GF, Xu ZF, Zhang X, Ping FY, Zhao SF (2005) Detection of telomerase activity in saliva from oral squamous cell carcinoma patient. Int J Oral Maxillofac Surg 34:566–570PubMedCrossRef

21.

Balicki R, Grabowskaa SZ, Citkob A (2005) Salivary epidermal growth factor in oral cavity cancer. Oral Oncol 41:48–55PubMedCrossRef

22.

Nagler R, Bahar G, Shpitzer T, Feinmesser R (2006) Concomitant analysis of salivary tumor markers—a new diagnostic tool for oral cancer. Clin Cancer Res 12:3979–3984PubMedCrossRef

23.

Pickering V, Jordan RCK, Schmidt BL (2007) Elevated salivary endothelin levels in oral cancer patients—a pilot study. Oral Oncol 43:37–41PubMedCrossRef

24.

Rhodus NL, Cheng B, Myers S, Miller L, Ho V, Ondrey F (2005) The feasibility of monitoring NF-κB associated cytokines: TNF-α, IL-1α, IL-6, and IL-8 in whole saliva for the malignant transformation of oral lichen planus. Mol Carcinog 44:77–82PubMedCrossRef

25.

Chaiyarit P, Ultrawichian A, Leelayuwat C, Vatanasapt P, Chcnchareonsook N, Samson MH, Giraud AS (2011) Investigation of trefoil factor expression in saliva and oral mucosal tissues of patients with oral squamous cell carcinoma. Clin Oral Investig. doi:10.1007/s00784-011-0667-z

26.

Rai B, Kaur J, Jacobs R, Anand SC (2011) Adenosine deaminase in saliva as a diagnostic marker of squamous cell carcinoma. Clin Oral Investig 15:347–349PubMedCrossRef

27.

Belperio JA, Keane MP, Arenberg DA, Addison CL, Ehlert JE, Burdick MD, Strieter RM (2000) CXC chemokines in angiogenesis. J Leukoc Biol 68:1–6PubMed

28.

Richards BL, Eisma RJ, Spiro JD, Lindquist RL, Kreutzer DL (1997) Co-expression of interleukin-8 receptors in head and neck squamous cell carcinoma. Am J Surg 174:507–512PubMedCrossRef

29.

Greenberg MS, Glick M, Ship JA (2008) Burkets oral medicine. BC Decker, Ontario

30.

Greenberg MS, Glick M (2003) Burket’s oral medicine diagnosis and treatment. BC Decker, Ontario

31.

Navazesh M, Christensen CM (1982) A comparison of whole mouth resting and stimulated salivary measurement procedures. J Dent Res 61:1158–1162PubMedCrossRef

32.

Balkwill F (2004) Cancer and the chemokine network. Nat Rev Cancer 4:540–545PubMedCrossRef

33.

Strieter RM, Burdick MD, Mestas J, Gomperts B, Keane MP, Belperio JA (2006) Cancer CXC chemokine networks and tumour angiogenesis. Eur J Cancer 42:768–778PubMedCrossRef

34.

Yeudall WA, Miyazaki H (2007) Chemokines and squamous cancer of the head and neck: targets for therapeutic intervention. Expert Rev Anticancer Ther 7:351–360PubMedCrossRef

35.

Bailey C, Negus R, Morris A, Ziprin P, Goldin R, Allavena P et al (2007) Chemokine expression is associated with the accumulation of tumour associated macrophages (TAMs) and progression in human colorectal cancer. Clin Exp Metastasis 24:121–130PubMedCrossRef

36.

Varney ML, Johansson SL, Singh RK (2006) Distinct expression of CXCL8 and its receptors CXCR1 and CXCR2 and their association with vessel density and aggressiveness in malignant melanoma. Am J Clin Pathol 125:209–216PubMed

37.

Brat DJ, Bellail AC, Van Meir EG (2005) The role of interleukin-8 and its receptors in gliomagenesis and tumoral angiogenesis. Neuro Oncol 7:122–133PubMedCrossRef

38.

Son DS, Parl AK, Rice VM, Khabele D (2007) Keratinocyte chemoattractant (kc)/human growth-regulated oncogene (gro) chemokines and pro-inflammatory chemokine networks in mouse and human ovarian epithelial cancer cells. Cancer Biol Ther 6:1302–1312PubMedCrossRef

39.

Mandel ID (1993) Salivary diagnosis: more than a lick and a promise. J Am Dent Assoc 124:85–87PubMed

40.

Ramsey PS, Andrews WW (2003) Biochemical predictors of preterm labor: fetal fibronectin and salivary estriol. Clin Perinatol 30:701–733PubMedCrossRef

41.

Hodinka R, Nagashunmugam T, Malamud D (1998) Detection of human immunodeficiency virus antibodies in oral fluids. Clin Diagn Lab Immunol 5:419–426PubMed

42.

Mortimer PP, Parry JV (1994) Detection of antibody to HIV in saliva: a brief review. Clin Diagn Virol 2:231–243PubMedCrossRef

43.

Mager DL (2005) The salivary microbiota as a diagnostic indicator of oral cancer: a descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects. J Transl Med 3:27PubMedCrossRef

44.

Rosas SL, Koch W, da Costa Carvalho MG (2001) Promoter hypermethylation patterns of p16, O6-methylguanine-DNAmethyl-transferase, and death-associated protein kinase in tumors and saliva of head and neck cancer patients. Cancer Res 61:939–942PubMed

45.

Jiang WW, Masayesva B, Zahurak M et al (2005) Increased mitochondrial DNA content in saliva associated with head and neck cancer. Clin Cancer Res 11:2486–2491PubMedCrossRef

46.

Miller SM (1994) Saliva testing: a nontraditional diagnostic tool. Clin Lab Sci 7:39–44PubMed

47.

Wong DT (2006) Salivary diagnostics powered by nanotechnologies, proteomics and genomics. J Am Dent Assoc 137:313–321PubMed

48.

Cohen RF, Contrino J, Spiro JD, Mann EA, Chen LL, Kreutzer DL (1995) Interleukin-8 expression by head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 121:202–209PubMedCrossRef

49.

Chen Z, Malhotra PS, Thomas GS, Ondrey FG, Duffey DC, Smith CW et al (1999) Expression of proinflammatory and proangiogenic cytokines in patients with head and neck cancer. Clin Cancer Res 5:1369–1379PubMed

50.

St John MAR, Li Y, Zhou X, Denny P, Ho CM, Montemagno C et al (2004) Interleukin 6 and interleukin 8 as potential biomarkers for oral cavity and oropharyngeal squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 130:929–935PubMedCrossRef

51.

Katakura A, Kamiyama I, Takano N, Shibahara T, Muramatsu T, Ishihara K et al (2007) Comparison of salivary cytokine levels in oral cancer patients and healthy subjects. Bull Tokyo Dent Coll 48:199–203PubMedCrossRef

52.

SahebJamee M, Eslami M, Moghadam FA, Sarafnejad A (2008) Salivary concentration of TNFα, IL1α, IL6, and IL8 in oral squamous cell carcinoma. Med Oral Patol Oral Cir Bucal 13:292–295

53.

Khan A, Farah CS, Savage NW, Walsh LJ, Harbrow DJ, Sugerman PB (2003) Th1 cytokines in oral lichen planus. J Oral Pathol Med 32:77–83PubMedCrossRef

54.

Haque MF, Meghji S, Khitab S, Harris M (2000) Oral submucous fibrosis patients have altered levels of cytokine production. J Oral Pathol Med 20:123–128CrossRef

55.

Brailo V, Boras VV, Arambasin AC, Alajbeg IZ, Milenovic A, Lukac J (2006) The significance of salivary interleukin 6 and tumor necrosis factor alpha in patients with oral leukoplakia. Oral Oncol 42:370–373PubMedCrossRef

Title: Salivary level of interleukin-8 in oral precancer and oral squamous cell carcinoma
Authors: Silky Rajesh Punyani
Ramhari Shankarrao Sathawane
Publication date: 01-03-2013
Publisher: Springer-Verlag
Published in: Clinical Oral Investigations / Issue 2/2013
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-012-0723-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Salivary level of interleukin-8 in oral precancer and oral squamous cell carcinoma

Abstract

Background

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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