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Clinical Oral Investigations

Published in:

01-10-2012 | Original Article

Assessment of lipopolysaccharide microleakage at conical implant-abutment connections

Authors: Sönke Harder, Elgar Susanne Quabius, Lars Ossenkop, Matthias Kern

Published in: Clinical Oral Investigations | Issue 5/2012

Abstract

Objective

The aim of this in vitro study was to assess lipopolysaccharide microleakage at conical implant-abutment connections of two-piece dental implants in terms of the expression levels of genes involved in lipopolysaccharide-mediated proinflammatory cytokine production.

Materials and methods

Two implant systems with conical implant-abutment connections were inoculated with lipopolysaccharide and submerged in human whole blood. Positive-control blood samples (without implants) were stimulated with 4 μg/ml, 2 μg/ml, 200 ng/ml, and 20 ng/ml lipopolysaccharide. Sampling was performed after 1, 8, and 24 h of incubation. Changes of gene expression levels of Toll-like receptor 9, tumor necrosis factor-α, nuclear factor kappa light chain enhancer of activated B cells, interleukin-1β, and interferon-γ were assessed by real-time quantitative PCR. In addition, protein expression levels of interleukin-6, tumor necrosis factor-α, interleukin-1β, and interferon-γ were determined by immunoassay.

Results

Changes in cytokine expression at the genomic and proteomic levels indicated lipopolysaccharide leakage at the interfaces of both tested implant systems, although some implants showed no sign of microleakage. Any tested concentration of lipopolysaccharide stimulated similar gene expression.

Conclusions

Conical implant-abutment connections of two-piece dental implants do not prevent microleakage on a molecular level. Changes in lipopolysaccharide-induced proinflammatory cytokine gene expression facilitate the detection of lipopolysaccharide microleakage at implant-abutment interfaces.

Clinical relevance

Small amounts of lipopolysaccharide released from intra-implant cavities can stimulate a detectable immunological response in human whole blood and may induce alveolar bone resorption via the osteoclast-activating pathway.

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Title: Assessment of lipopolysaccharide microleakage at conical implant-abutment connections
Authors: Sönke Harder
Elgar Susanne Quabius
Lars Ossenkop
Matthias Kern
Publication date: 01-10-2012
Publisher: Springer-Verlag
Published in: Clinical Oral Investigations / Issue 5/2012
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-011-0646-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Assessment of lipopolysaccharide microleakage at conical implant-abutment connections

Abstract

Objective

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Materials and methods

Results

Conclusions

Clinical relevance

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