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Clinical Oral Investigations
Published in: Clinical Oral Investigations 2/2005

01-06-2005 | Original Article

Influence of different treatment approaches on the removal of early plaque biofilms and the viability of SAOS2 osteoblasts grown on titanium implants

Authors: Frank Schwarz, Anton Sculean, Georg Romanos, Monika Herten, Nadine Horn, Werner Scherbaum, Jürgen Becker

Published in: Clinical Oral Investigations | Issue 2/2005

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Abstract

The aim of the present study was to evaluate the influence of different treatment approaches on: (1) the removal of early plaque biofilms grown on titanium implants, and (2) the biocompatibility of the instrumented implant surfaces. Five volunteers wore acrylic splints with sand-blasted and acid-etched titanium discs for 24 h to build up supragingival plaque. A total of 80 specimens were randomly assigned to the following groups: (1) an Er:YAG laser (100 mJ/pulse, 10 Hz) (Y), (2) an ultrasonic system (U), (3) plastic curettes and rinsing with chlorhexidine digluconate (P), or (4) unworn titanium discs (C). Autoclaved specimens were incubated with SAOS2 cells for three days. The following parameters were measured: treatment time (T), residual plaque biofilm (RPB) and clean implant surface (CIS) areas (%), and mitochondrial cell activity (MA) (counts/s). Statistical analysis within and between groups revealed the following mean scores (±SD): RPB areas: P (61.1±11.4) > U (36.8±4.5) > Y (5.8±5.1); CIS areas: Y (94.2±5.1) > U (63.2±4.5) > P (38.9±11.2); T: Y (5.6±1.2) > U (2.4±0.5) > P (2.3±0.5); MA: C (1.528.636±188.371) > U (831.594±370.228) > Y (678.250±367.902) > P (144.105±120.961). Within the limits of the present study, it may be concluded that Y seems to be most suitable for the removal of supragingival early plaque biofilms grown on SLA titanium implants, and (2) all treatment procedures failed to restore the biocompatibility of previously-contaminated SLA titanium surfaces.
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Metadata
Title
Influence of different treatment approaches on the removal of early plaque biofilms and the viability of SAOS2 osteoblasts grown on titanium implants
Authors
Frank Schwarz
Anton Sculean
Georg Romanos
Monika Herten
Nadine Horn
Werner Scherbaum
Jürgen Becker
Publication date
01-06-2005
Publisher
Springer-Verlag
Published in
Clinical Oral Investigations / Issue 2/2005
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-005-0305-8

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