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01-11-2014 | Original Article

Synergistic and sequential effects of BMP-2, bFGF and VEGF on osteogenic differentiation of rat osteoblasts

Authors: Peipei Li, Yan Bai, Guangfu Yin, Ximing Pu, Zhongbing Huang, Xiaoming Liao, Xianchun Chen, Yadong Yao

Published in: Journal of Bone and Mineral Metabolism | Issue 6/2014

Abstract

In the present study, the effects of bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on regulation of rat osteoblast (ROB) maturation in vitro were investigated. It was found that the proliferation, differentiation and mineralization of ROBs were all dose-dependently increased at particular times in the case of treatment with only one growth factor. To investigate the effects of combined treatment, ROBs were treated with either a single application of a relatively high dose of each growth factor, or binary/triple combined applications of relatively low doses of the growth factors. Osteogenic differentiation was significantly promoted in the triple combination treatment of BMP-2, VEGF and bFGF compared with the single or binary combination treatments. The optimal timing of the triple combination to enhance osteogenesis was also tested. When bFGF and VEGF were added in the early stage, and BMP-2 and VEGF were added in the late stage, osteogenic differentiation of ROBs could be enhanced more effectively. These results could be used to construct bone tissue engineering scaffolds that release growth factors sequentially.

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Title: Synergistic and sequential effects of BMP-2, bFGF and VEGF on osteogenic differentiation of rat osteoblasts
Authors: Peipei Li
Yan Bai
Guangfu Yin
Ximing Pu
Zhongbing Huang
Xiaoming Liao
Xianchun Chen
Yadong Yao
Publication date: 01-11-2014
Publisher: Springer Japan
Published in: Journal of Bone and Mineral Metabolism / Issue 6/2014
Print ISSN: 0914-8779
Electronic ISSN: 1435-5604
DOI: https://doi.org/10.1007/s00774-013-0538-6

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