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Archives of Virology
Published in: Archives of Virology 6/2019

01-06-2019 | Annotated Sequence Record

Molecular characterisation of a putative new polerovirus infecting pumpkin (Cucurbita pepo) in Kenya

Authors: Dawit B. Kidanemariam, Amit C. Sukal, Adane D. Abraham, Joyce N. Njuguna, Francesca Stomeo, James L. Dale, Robert M. Harding, Anthony P. James

Published in: Archives of Virology | Issue 6/2019

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Abstract

Next-generation sequencing of RNA extracted from a pumpkin plant with mosaic symptoms in Kenya identified the presence of a polerovirus sequence closely related to pepo aphid-borne yellows virus (PABYV). The near-complete polerovirus sequence comprised 5,810 nucleotides and contained seven putative open reading frames (ORFs) with a genome organisation typical of poleroviruses. BLASTp analysis of the translated sequences of ORFs 0, 1 and 2 revealed that their amino acid sequences differed by more than 10% from the corresponding protein sequences of other poleroviruses. These results suggest that this virus is a putative novel member of the genus Polerovirus, which has been provisionally named “pumpkin polerovirus” (PuPV).
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Metadata
Title
Molecular characterisation of a putative new polerovirus infecting pumpkin (Cucurbita pepo) in Kenya
Authors
Dawit B. Kidanemariam
Amit C. Sukal
Adane D. Abraham
Joyce N. Njuguna
Francesca Stomeo
James L. Dale
Robert M. Harding
Anthony P. James
Publication date
01-06-2019
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 6/2019
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-019-04219-8

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