Top

Published in:

01-03-2015 | Annotated Sequence Record

Identification and sequence determination of a novel double-stranded RNA mycovirus from the entomopathogenic fungus Beauveria bassiana

Authors: Ioly Kotta-Loizou, Jana Sipkova, Robert H. A. Coutts

Published in: Archives of Virology | Issue 3/2015

Abstract

An isolate of the entomopathogenic fungus Beauveria bassiana was found to contain five double-stranded (ds) RNA elements ranging from 1.5 to more than 3 kbp. The complete sequence of the largest dsRNA element is described here. Analysis of the RdRp nucleotide sequence reveals its similarity to unclassified dsRNA elements, such as Alternaria longipes dsRNA virus 1, and its distant relationship to the RNA-dependent RNA polymerases of members of the family Partitiviridae.

Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402CrossRefPubMedCentralPubMed

Banks GT, Buck KW, Chain EB, Darbyshire JE, Himmelweit F (1969) Virus-like particles in penicillin producing strains of Penicillium chrysogenum. Nature 223:89–90CrossRef

Coutts RHA, Livieratos IC (2003) A rapid method for sequencing the 5′- and 3′-termini of dsRNA viral templates using RLM-RACE. J Phytopathol 151:525–527CrossRef

Froussard P (1992) A random-PCR method (rPCR) to construct whole cDNA library from low amounts of RNA. Nucleic Acids Res 20:2900CrossRefPubMedCentralPubMed

Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384CrossRefPubMed

Ghikas DV, Kouvelis VN, Typas MA (2010) Phylogenetic and biogeographic implications inferred by mitochondrial intergenic region analyses and ITS1-5.8S-ITS2 of the entomopathogenic fungi Beauveria bassiana and B. brongniartii. BMC Microbiol 10:174CrossRefPubMedCentralPubMed

Kwon S-J, Lim W-S, Park S-H, Park M-R, Kim K-H (2009) Molecular characterization of a dsRNA mycovirus, Fusarium graminearum virus-DK21, which is phylogenetically related to hypoviruses but has a genome organization and gene expression strategy resembling those of plant potex-like viruses. Mol Cells 28:73–74CrossRef

Herrero N, Duenas E, Quesada-Moraga E, Zabalgogeazcoa I (2012) Prevalence and diversity of viruses in the entomopathogenic fungus Beauveria bassiana. Appl Environ Microbiol 78:8523–8530CrossRefPubMedCentralPubMed

Lin Y, Zhang H, Zhao C, Liu S, Guo L (2014) The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28. Arch Virol. doi:10.1007/s00705-014-2218-9 PubMedCentral

10.

Liu H, Fu Y, Jiang D, Li G, Xie J, Peng Y, Yi X, Ghabrial SA (2009) A novel mycovirus that is related to the human pathogen hepatitis E virus and rubi-like viruses. J Virol 83:1981–1991CrossRefPubMedCentralPubMed

11.

Nuss DL (2005) Hypovirulence: mycoviruses at the fungal-plant interface. Nat Rev Microbiol 3:632–642CrossRefPubMed

12.

Yie SW, Khalifa ME, Hahn T, Pearson MN (2014) Molecular characterization of a novel victorivirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 159:1321–1327CrossRefPubMed

13.

Yu X, Li B, Fu Y, Jiang D, Ghabrial SA, Li G, Peng Y, Xie J, Cheng J, Huang J, Yi X (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci USA 107:8387–8392CrossRefPubMedCentralPubMed

14.

Zhang T, Jiang Y, Dong W (2014) A novel monopartite dsRNA virus isolated from the phytopathogenic fungus Ustilaginoidea virens and ancestrally related to a mitochondria-associated dsRNA in the green alga Bryopsis. Virology. doi:10.1016/j.virol.2014.06.003

15.

Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406–3415CrossRefPubMedCentralPubMed

Title: Identification and sequence determination of a novel double-stranded RNA mycovirus from the entomopathogenic fungus Beauveria bassiana
Authors: Ioly Kotta-Loizou
Jana Sipkova
Robert H. A. Coutts
Publication date: 01-03-2015
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 3/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-014-2332-8

Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin

Prof. Florian Limbourg

Prof. Anoop Chauhan

Developed by: Springer Medicine

Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2015

Regulation of the microRNA processor DGCR8 by hepatitis B virus proteins via the transcription factor YY1

The gene expression profile of porcine alveolar macrophages infected with a highly pathogenic porcine reproductive and respiratory syndrome virus indicates overstimulation of the innate immune system by the virus

Complete genome sequence of a filamentous bacteriophage, RS611, that infects the phytopathogen Ralstonia solanacearum

Identification of a picornavirus from chickens with transmissible viral proventriculitis using metagenomic analysis

Qualitative differences in brain-infiltrating T cells are associated with a fatal outcome in mice infected with Japanese encephalitis virus

A -819 C/T polymorphism in the interleukin-10 promoter is associated with persistent HBV infection, but -1082 A/G and -592A/C polymorphisms are not: a meta-analysis

Keynote webinar | Spotlight on medication adherence

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

At a glance: The STEP trials