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Archives of Virology
Published in: Archives of Virology 2/2015

01-02-2015 | Annotated Sequence Record

The complete nucleotide sequence of chrysanthemum stem necrosis virus

Authors: A. M. Dullemans, J. Th. J. Verhoeven, R. Kormelink, R. A. A. van der Vlugt

Published in: Archives of Virology | Issue 2/2015

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Abstract

The complete genome sequence of chrysanthemum stem necrosis virus (CSNV) was determined using Roche 454 next-generation sequencing. CSNV is a tentative member of the genus Tospovirus within the family Bunyaviridae, whose members are arthropod-borne. This is the first report of the entire RNA genome sequence of a CSNV isolate. The large RNA of CSNV is 8955 nucleotides (nt) in size and contains a single open reading frame of 8625 nt in the antisense arrangement, coding for the putative RNA-dependent RNA polymerase (L protein) of 2874 aa with a predicted Mr of 331 kDa. Two untranslated regions of 397 and 33 nt are present at the 5’ and 3’ termini, respectively. The medium (M) and small (S) RNAs are 4830 and 2947 nt in size, respectively, and show 99 % identity to the corresponding genomic segments of previously partially characterized CSNV genomes. Protein sequences for the precursor of the Gn/Gc proteins, N and NSs, are identical in length in all of the analysed CSNV isolates.
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Metadata
Title
The complete nucleotide sequence of chrysanthemum stem necrosis virus
Authors
A. M. Dullemans
J. Th. J. Verhoeven
R. Kormelink
R. A. A. van der Vlugt
Publication date
01-02-2015
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 2/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-014-2282-1

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