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01-01-2015 | Brief Report

Identification of several clades of novel single-stranded circular DNA viruses with conserved stem-loop structures in pig feces

Authors: Andrew K. Cheung, Terry Fei Fan Ng, Kelly M. Lager, David P. Alt, Eric Delwart, Roman M. Pogranichniy

Published in: Archives of Virology | Issue 1/2015

Abstract

Metagenomic analysis of fecal samples collected from diarrheal swine detected sequences encoding a replication initiator protein (Rep). The genomes of ten novel single-stranded DNA viruses were determined, and they exhibited a similar genome organization. The two putative open reading frames (ORFs) encoding Rep and the capsid protein are bidirectionally transcribed and separated by two intergenic regions. Stem-loop structure(s) typical of genomes that undergo the rolling-circle DNA replication mechanism were observed. Phylogenetically, these ten genomes are in a monophyletic clade with the previously described porcine stool-associated virus (PoSCV) but are divergent enough to be further classified into to six distinct virus clades.

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Title: Identification of several clades of novel single-stranded circular DNA viruses with conserved stem-loop structures in pig feces
Authors: Andrew K. Cheung
Terry Fei Fan Ng
Kelly M. Lager
David P. Alt
Eric Delwart
Roman M. Pogranichniy
Publication date: 01-01-2015
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 1/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-014-2234-9

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