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Archives of Virology
Published in: Archives of Virology 8/2013

01-08-2013 | Brief Report

Genetic diversity and evolutionary characterization of Chinese porcine reproductive and respiratory syndrome viruses based on NSP2 and ORF5

Authors: Jian-Kui Liu, Chun-Hua Wei, Xiao-Yan Yang, Xi-Lin Hou, AI-Ling Dai, Xiao-Hua Li, Mei-Kang Wei, Xiu-Zhen Pan

Published in: Archives of Virology | Issue 8/2013

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Abstract

To more fully understand the extent of genetic diversity of PRRSV in China, we analyzed the Nsp2 and ORF5 gene sequences of 35 representative PRRSV isolates from 2008 to 2012. Sequence analysis revealed that the Nsp2 and ORF5 genes have undergone genetic variation. Furthermore, the isolate FJLYDX04 contains five insertions at positions 599 to 603 and is the first isolate from China reported to have an insertion in Nsp2. Our results suggest that the highly pathogenic PRRSV has become the dominant strain in China and that Chinese PRRSV has undergone rapid evolution and can circumvent immune responses induced by currently used vaccines.
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Literature
1.
Neumann EJ, Kliebenstein JB, Johnson CD, Mabry JW, Bush EJ, Seitzinger AH, Green AL, Zimmerman JJ (2005) Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States. J Am Vet Med Assoc 227:385–392PubMedCrossRef
2.
Pejsak Z, Stadejek T, Markowska-Daniel I (1997) Clinical signs and economic losses caused by porcine reproductive and respiratory syndrome virus in a large breeding farm. Vet Microbiol 55:317–322PubMedCrossRef
3.
Tian K, Yu X, Zhao T, Feng Y, Cao Z, Wang C, Hu Y, Chen X, Hu D, Tian X, Liu D, Zhang S, Deng X, Ding Y, Yang L, Zhang Y, Xiao H, Qiao M, Wang B, Hou L, Wang X, Yang X, Kang L, Sun M, Jin P, Wang S, Kitamura Y, Yan J, Gao GF (2007) Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PLoS ONE 2:e526PubMedCrossRef
4.
Stadejek T, Stankevicius A, Storgaard T, Oleksiewicz MB, Belak S, Drew TW, Pejsak Z (2002) Identification of radically different variants of porcine reproductive and respiratory syndrome virus in Eastern Europe: towards a common ancestor for European and American viruses. J Gen Virol 83:1861–1873PubMed
5.
Bautista EM, Meulenberg JJ, Choi CS, Molitor TW (1996) Structural polypeptides of the American (VR-2332) strain of porcine reproductive and respiratory syndrome virus. Arch Virol 141:1357–1365PubMedCrossRef
6.
Meulenberg JJ, Petersen-den Besten A, De Kluyver EP, Moormann RJ, Schaaper WM, Wensvoort G (1995) Characterization of proteins encoded by ORFs 2 to 7 of Lelystad virus. Virology 206:155–163PubMedCrossRef
7.
Nelsen CJ, Murtaugh MP, Faaberg KS (1999) Porcine reproductive and respiratory syndrome virus comparison: divergent evolution on two continents. J Virol 73:270–280PubMed
8.
Meng XJ (2000) Heterogenicity of porcine reproductive and respiratory syndrome virus: implication for current vaccine efficacy and future vaccine development. Vet Microbiol 74:309–329PubMedCrossRef
9.
Fang Y, Kim DY, Ropp S, Steen P, Christopher-Hennings J, Nelson EA, Rowland RR (2004) Heterogeneity in Nsp2 of European-like porcine reproductive and respiratory syndrome viruses isolated in the United States. Virus Res 100:229–235PubMedCrossRef
10.
Oleksiewicz MB, Botner A, Toft P, Grubbe T, Nielsen J, Kamstrup S, Storgaard T (2000) Emergence of porcine reproductive and respiratory syndrome virus deletion mutants: correlation with the porcine antibody response to a hypervariable site in the ORF 3 structural glycoprotein. Virology 267:135–140PubMedCrossRef
11.
Murtaugh MP, Elam MR, Kakach LT (1995) Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus. Arch Virol 140:1451–1460PubMedCrossRef
12.
Zhou L, Zhang J, Zeng J, Yin S, Li Y, Zheng L, Guo X, Ge X, Yang H (2009) The 30-amino-acid deletion in the Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence. J Virol 83:5156–5167PubMedCrossRef
13.
Li B, Fang LR, Liu SY, Zhao FW, Jiang YB, He KW, Chen HC, Xiao SB (2010) The genomic diversity of Chinese porcine reproductive and respiratory syndrome virus isolates from 1996 to 2009. Vet Microbiol 146:226–237PubMedCrossRef
14.
Barfoed AM, Blixenkrone-Moller M, Jensen MH, Botner A, Kamstrup S (2004) DNA vaccination of pigs with open reading frame 1–7 of PRRS virus. Vaccine 22:3628–3641PubMedCrossRef
15.
de Lima M, Pattnaik AK, Flores EF, Osorio FA (2006) Serologic marker candidates identified among B-cell linear epitopes of Nsp2 and structural proteins of a North American strain of porcine reproductive and respiratory syndrome virus. Virology 353:410–421PubMedCrossRef
16.
Oleksiewicz MB, Bøtner A, Toft P, Normann P, Storgaard T (2001) Epitope mapping porcine reproductive and respiratory syndrome virus by phage display: the nsp2 fragment of the replicase polyprotein contains a cluster of B-cell epitopes. J Virol 75:3277–3290PubMedCrossRef
17.
Yan Y, Guo X, Ge X, Chen Y, Cha Z, Yang H (2007) Monoclonal antibody and porcine antisera recognized B-cell epitopes of Nsp2 protein of a Chinese strain of porcine reproductive and respiratory syndrome virus. Virus Res 126:207–215PubMedCrossRef
18.
Li B, Fang LR, Xu ZF, Liu SY, Gao JF, Jiang YB, Chen HC, Xiao SB (2009) Recombination in vaccine and circulating strains of porcine reproductive and respiratory syndrome viruses. Emerg Infect Dis 15:2032–2035PubMedCrossRef
19.
Ostrowski M, Galeota JA, Jar AM, Platt KB, Osorio FA, Lopez OJ (2002) Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. J Virol 76:4241–4250PubMedCrossRef
20.
Ansari IH, Kwon BJ, Osorio FA, Pattnaik AK (2006) Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies. J Virol 80:3994–4004PubMedCrossRef
21.
Madsen KG, Hansen CM, Madsen ES, Strandbygaard B, Botner A, Sorensen KJ (1998) Sequence analysis of porcine reproductive and respiratory syndrome virus of the American type collected from Danish swineherds. Arch Virol 143:1683–1700PubMedCrossRef
22.
Yang SX, Kwang J, Laegreid W (1998) Comparative sequence analysis of open reading frames 2 to 7 of the modified live vaccine virus and other North American isolates of the porcine reproductive and respiratory syndrome virus. Arch Virol 143:601–612PubMedCrossRef
23.
Allende R, Kutish GF, Laegreid W, Lu Z, Lewis TL, Rock DL, Friesen J, Galeota JA, Doster AR, Osorio FA (2000) Mutation in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype. Arch Virol 145:1149–1161PubMedCrossRef
24.
Wesley RD, Mengeling WL, Lager KM, Vorwald AC, Roof MB (1999) Evidence for divergence of restriction fragment length polymorphism patterns following in vivo replication of porcine reproductive and respiratory syndrome virus. Am J Vet Res 60:463–467PubMed
Metadata
Title
Genetic diversity and evolutionary characterization of Chinese porcine reproductive and respiratory syndrome viruses based on NSP2 and ORF5
Authors
Jian-Kui Liu
Chun-Hua Wei
Xiao-Yan Yang
Xi-Lin Hou
AI-Ling Dai
Xiao-Hua Li
Mei-Kang Wei
Xiu-Zhen Pan
Publication date
01-08-2013
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 8/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-013-1669-8

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