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Archives of Virology
Published in: Archives of Virology 6/2012

01-06-2012 | Brief Report

Diverse population of a new bipartite begomovirus infecting tomato crops in Uruguay

Authors: Belén Márquez-Martín, Diego Maeso, Ada Martínez-Ayala, Roberto Bernal, M. Teresa Federici, Paul Vincelli, Jesús Navas-Castillo, Enrique Moriones

Published in: Archives of Virology | Issue 6/2012

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Abstract

Several isolates of a novel begomovirus were characterized from tomato samples collected in northern Uruguay exhibiting disease symptoms associated with Bemisia tabaci infestations. Analysis of full-length sequences of DNA-A and DNA-B components revealed the presence of a new begomovirus with the typical genome organization of a New World begomovirus, for which the name tomato rugose yellow leaf curl virus (ToRYLCV) is proposed. A high degree of nucleotide sequence diversity was found for both components, suggesting the presence of a diverse virus population. Recombination analysis suggested relationships of ToRYLCV to begomoviruses reported from the New World. Although common regions from DNA-As and DNA-Bs were surprisingly divergent for a cognate pair, a DNA-A and DNA-B pair cloned from one sample were infectious in Nicotiana benthamiana and tomato and reproduced symptoms observed in field-infected tomato plants, suggesting that ToRYLCV is the causal agent of the disease observed. This is the first report of a begomovirus infecting tomato crops in Uruguay and of the presence of begomovirus in this country.
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Metadata
Title
Diverse population of a new bipartite begomovirus infecting tomato crops in Uruguay
Authors
Belén Márquez-Martín
Diego Maeso
Ada Martínez-Ayala
Roberto Bernal
M. Teresa Federici
Paul Vincelli
Jesús Navas-Castillo
Enrique Moriones
Publication date
01-06-2012
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 6/2012
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1262-6

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