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Published in: Archives of Virology 8/2011

01-08-2011 | Annotated Sequence Record

First report of the complete sequence of Sida golden yellow vein virus from Jamaica

Authors: Cheryl S. Stewart, Tatsuya Kon, Robert L. Gilbertson, Marcia E. Roye

Published in: Archives of Virology | Issue 8/2011

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Abstract

Begomoviruses are phytopathogens that threaten food security [18]. Sida spp. are ubiquitous weed species found in Jamaica. Sida samples were collected island-wide, DNA was extracted via a modified Dellaporta method, and the viral genome was amplified using degenerate and sequence-specific primers [2, 11]. The amplicons were cloned and sequenced. Sequence analysis revealed that a DNA-A molecule isolated from a plant in Liguanea, St. Andrew, was 90.9% similar to Sida golden yellow vein virus-[United States of America:Homestead:A11], making it a strain of SiGYVV. It was named Sida golden yellow vein virus-[Jamaica:Liguanea 2:2008] (SiGYVV-[JM:Lig2:08]). The cognate DNA-B, previously unreported, was successfully cloned and was most similar to that of Malvastrum yellow mosaic Jamaica virus (MaYMJV). Phylogenetic analysis suggested that this virus was most closely related to begomoviruses that infect malvaceous hosts in Jamaica, Cuba and Florida in the United States.
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Metadata
Title
First report of the complete sequence of Sida golden yellow vein virus from Jamaica
Authors
Cheryl S. Stewart
Tatsuya Kon
Robert L. Gilbertson
Marcia E. Roye
Publication date
01-08-2011
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 8/2011
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-011-1030-z

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