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Open Access 01-03-2010 | Brief Report

A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia

Authors: Afshin Hassani-Mehraban, Marleen Botermans, J. Th. J. Verhoeven, Ellis Meekes, Janneke Saaijer, Dick Peters, Rob Goldbach, Richard Kormelink

Published in: Archives of Virology | Issue 3/2010

Abstract

A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests. Further analysis revealed a close serological relationship to tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV). A major part of the S-RNA segment, encompassing the nucleocapsid (N) protein gene, the 5′ untranslated region and a part of the intergenic region 3′ of the N gene, was cloned and sequenced. The deduced N protein sequence showed highest amino acid identity (82%) to that of TCSV, indicating that the virus represents a new tospovirus species, for which the name Alstroemeria necrotic streak virus (ANSV) is coined. Phylogenetic analysis based on the N protein sequence revealed that this Alstroemeria-infecting tospovirus clustered with tospoviruses from the American continent. Frankliniella occidentalis was identified as potential vector species for ANSV.

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Title: A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia
Authors: Afshin Hassani-Mehraban
Marleen Botermans
J. Th. J. Verhoeven
Ellis Meekes
Janneke Saaijer
Dick Peters
Rob Goldbach
Richard Kormelink
Publication date: 01-03-2010
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 3/2010
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-010-0590-7

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