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Published in: Archives of Virology 9/2009

01-09-2009 | Original Article

An improved reverse transcription loop-mediated isothermal amplification assay for sensitive and specific detection of Newcastle disease virus

Authors: Qiang Li, Chunyi Xue, Jianping Qin, Qingfeng Zhou, Feng Chen, Yingzuo Bi, Yongchang Cao

Published in: Archives of Virology | Issue 9/2009

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Abstract

Five of 21 different Newcastle disease virus (NDV) strains isolated from China in 2007 gave false-negative results when detected with a previously reported loop-mediated isothermal amplification (LAMP) method, and mismatches were found between the target gene and LAMP primers. Therefore, an improved, sensitive, specific, and accelerated one-step reverse transcription (RT)-LAMP assay with degenerate primers was developed. Our data demonstrated that the improved RT-LAMP assay detected all 21 NDV isolates, had no cross-reaction with three other avian viruses, could be performed in 50 min less time, was 5-fold more sensitive than the previous LAMP assay, and achieved 96.8% sensitivity with 62 samples, including 30 field clinical samples, 24 experimentally infected samples, and 8 experimentally negative samples. Therefore, the improved RT-LAMP assay is a simple, rapid, and cost-effective method that is practical for less well-equipped laboratories and in the field.
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Metadata
Title
An improved reverse transcription loop-mediated isothermal amplification assay for sensitive and specific detection of Newcastle disease virus
Authors
Qiang Li
Chunyi Xue
Jianping Qin
Qingfeng Zhou
Feng Chen
Yingzuo Bi
Yongchang Cao
Publication date
01-09-2009
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 9/2009
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-009-0464-z

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