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01-01-2010 | Basic Neurosciences, Genetics and Immunology - Rapid Communication

Astrocytes and Glioblastoma cells release exosomes carrying mtDNA

Authors: Michele Guescini, Susanna Genedani, Vilberto Stocchi, Luigi Francesco Agnati

Published in: Journal of Neural Transmission | Issue 1/2010

Abstract

Cells can exchange information not only by means of chemical and/or electrical signals, but also via microvesicles released into the intercellular space. The present paper, for the first time, provides evidence that Glioblastoma and Astrocyte cells release microvesicles, which carry mitochondrial DNA (mtDNA). These microvesicles have been characterised as exosomes in view of the presence of some protein markers of exosomes, such as Tsg101, CD9 and Alix. Thus, the important finding has been obtained that bonafide exosomes, constitutively released by Glioblastoma cells and Astrocytes, can carry mtDNA, which can be, therefore, transferred between cells. This datum may help the understanding of some diseases due to mitochondrial alterations.

Agnati LF, Guidolin D, Baluska F, Leo G, Barlow PW, Carone C, Genedani S (2009) A new hypothesis of pathogenesis based on the divorce between mitochondria and their host cells: possible relevances for the Alzheimer’s disease. Curr Alzheimer Res (in press)

Barral AM, von Herrath MG (2005) Exosomes: specific intercellular nano-shuttles. Curr Immunol Rev 1:1–6CrossRef

Belting M, Wittrup A (2008) Nanotubes, exosomes, and nucleic acid-binding peptides provide novel mechanisms of intercellular communication in eukaryotic cells: implications in health and disease. J Cell Biol 183:1187–1191CrossRefPubMed

Cocucci E, Racchetti G, Meldolesi J (2009) Shedding microvesicles: artefacts no more. Trends Cell Biol 19:43–51CrossRefPubMed

Fevrier B, Raposo G (2004) Exosomes: endosomal-derived vesicles shipping extracellular messages. Curr Opin Cell Biol 16:415–421CrossRefPubMed

Gerdes HH, Bukoreshtliev NV, Barroso JF (2007) Tunneling nanotubes: a new route for the exchange of components between animal cells. FEBS Lett 581:2194–2201CrossRefPubMed

Jansen FH, Krijgsveld J, van Rijswijk A, van den Bemd GJ, van den Berg MS, van Weerden WM, Willemsen R, Dekker LJ, Luider TM, Jenster G (2009) Exosomal secretion of cytoplasmic prostate cancer xenograft-derived proteins. Mol Cell Proteomics 8:1192–1205CrossRefPubMed

Lakkaraju A, Rodriguez-Boulan E (2008) Itinerant exosomes: emerging roles in cell and tissue polarity. Trends Cell Biol 18:199–209CrossRefPubMed

Mallegol J, Van Niel G, Lebreton C, Lepelletier Y, Candalh C, Dugave C, Heath JK, Raposo G, Cerf-Bensussan N, Heyman M (2007) T84-intestinal epithelial exosomes bear MHC class II/peptide complexes potentiating antigen presentation by dendritic cells. Gastroenterology 132:1866–1876CrossRefPubMed

Mears R, Craven RA, Hanrahan S, Totty N, Upton C, Young SL, Patel P, Selby PJ, Banks RE (2004) Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Proteomics 4:4019–4031CrossRefPubMed

Raiborg C, Rusten TE, Stenmark H (2003) Protein sorting into multivesicular endosomes. Curr Opin Cell Biol 15:446–455CrossRefPubMed

Rajendran L, Honsho M, Zahn TR, Keller P, Geiger KD, Verkade P, Simons K (2006) Alzheimer’s disease beta-amyloid peptides are released in association with exosomes. Proc Natl Acad Sci USA 103:11172–11177CrossRefPubMed

Rustom A, Saffrich R, Markovic I, Walther P, Gerdes HH (2004) Nanotubular highways for intercellular organelle transport. Science 303:1007–1010CrossRefPubMed

Schapira AH (2006) Mitochondrial disease. Lancet 368:70–82CrossRefPubMed

Schorey JS, Bhatnagar S (2008) Exosome function: from tumor immunology to pathogen biology. Traffic 9:871–881CrossRefPubMed

Simons M, Raposo G (2009) Exosomes—vesicular carriers for intercellular communication. Curr Opin Cell Biol May 11. [Epub ahead of print]

Skog J, Wurdinger T, van Rijn S, Meijer DH, Gainche L, Sena-Esteves M, Curry WT Jr, Carter BS, Krichevsky AM, Breakefield XO (2008) Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol 10:1470–1476CrossRefPubMed

Smalheiser NR (2007) Exosomal transfer of proteins and RNAs at synapses in the nervous system. Biol Direct 2:35CrossRefPubMed

Staubach S, Razawi H, Hanisch FG (2009) Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7. Proteomics 9:2820–2835CrossRefPubMed

van Niel G, Porto-Carreiro I, Simoes S, Raposo G (2006) Exosomes: a common pathway for a specialized function. J Biochem 140:13–21CrossRefPubMed

Title: Astrocytes and Glioblastoma cells release exosomes carrying mtDNA
Authors: Michele Guescini
Susanna Genedani
Vilberto Stocchi
Luigi Francesco Agnati
Publication date: 01-01-2010
Publisher: Springer Vienna
Published in: Journal of Neural Transmission / Issue 1/2010
Print ISSN: 0300-9564
Electronic ISSN: 1435-1463
DOI: https://doi.org/10.1007/s00702-009-0288-8

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Astrocytes and Glioblastoma cells release exosomes carrying mtDNA

Abstract

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Abstract

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