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01-04-2011 | Original Article

Comparative study on determination of plasma thyroid hormones by chemiluminescence and electrochemiluminescence immunoassay methods in sheep

Authors: Behrad Eshratkhah, Hamid Rajabian, Davar Namvar, Salar Eshratkhah, Salar Mohammadi Bastam

Published in: Comparative Clinical Pathology | Issue 2/2011

Abstract

The aim of this study was to compare plasma thyroid hormone concentrations by both chemiluminescence (CLIA) and electrochemiluminescence immunoassay (ECLIA) methods in sheep. Blood samples were taken from the jugular vein of 25 clinically healthy, non-pregnant adult ewes. The plasma was analyzed to determine thyroxine (T₄), tri-iodothyronine (T₃), free thyroxine (fT₄) and free tri-iodothyronine (fT₃) concentrations. The data from this study indicates, there were significant differences in the T₄ (P < 0.0001), T₃(P < 0.01) and fT₃(P < 0.01) concentrations between the two methods, and the levels of these hormones were higher when using the ECLIA method. In determining thyroid hormones using the CLIA and ECLIA methods, significant positive correlations were found between T₄ and fT₄ (P < 0.001, r = 0.703; P < 0.0001, r = 0.806) also between T₃ and fT₃ (P < 0.0001, r = 0.922; P < 0.0001, r = 0.923) concentrations, respectively. The linear regression analysis of these hormones showed that the CLIA and ECLIA results were significantly correlated (T₄ (P < 0.01, r = 0.583), T₃ (P < 0.0001, r = 0.898), fT4 (P < 0.0001, r = 0.796) and fT₃ (P < 0.0001, r = 0.898).

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Title: Comparative study on determination of plasma thyroid hormones by chemiluminescence and electrochemiluminescence immunoassay methods in sheep
Authors: Behrad Eshratkhah
Hamid Rajabian
Davar Namvar
Salar Eshratkhah
Salar Mohammadi Bastam
Publication date: 01-04-2011
Publisher: Springer-Verlag
Published in: Comparative Clinical Pathology / Issue 2/2011
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-010-0967-8

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Comparative study on determination of plasma thyroid hormones by chemiluminescence and electrochemiluminescence immunoassay methods in sheep

Abstract

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