Published in:
01-02-2010 | Original Article—Alimentary Tract
The 5-HT4 receptor agonist mosapride attenuates NSAID-induced gastric mucosal damage
Published in: Journal of Gastroenterology | Issue 2/2010
Login to get accessAbstract
Background
The cholinergic anti-inflammatory pathway is a novel physiological mechanism found at various locations in the body where the nicotinic regulation of inflammatory cells through the autonomic nervous system is involved. In this study, we tested the hypothesis that cholinergic nerve stimulation by a 5-HT4 agonist may modulate the progression of gastric mucosal ulcers induced by nonsteroidal anti-inflammatory drugs (NSAIDs).
Methods
Acute gastric ulcers were induced in rats by the oral administration of indomethacin.
Results
Gastric damage analysis indicated that pretreatment with mosapride, a selective 5-HT4 agonist, at 0.25, 0.5, and 0.75 mg/kg, inhibited the mucosal damage induced by indomethacin. In gastric emptying analysis, an evacuation effect was observed in the 3.0 mg/kg mosapride pretreatment group, but this effect was not observed in the lower dose (0.5 mg/kg) group. The antiulcerogenic activity of mosapride treatment (at 0.5 mg/kg) was blocked by a 5-HT4-specific antagonist, GR113808 (1 mg/kg, i.v.). Additionally, we demonstrated that methyllycaconitine (0.29 and 0.87 mg/kg i.p.), a selective inhibitor of α7 nicotinic acetylcholine (ACh) receptors (α7nAChRs), ablated the antiulcerogenic action of mosapride.
Conclusions
These results suggest that the mucosal protective action of mosapride may be mediated by an action on immune cells through the acceleration of ACh release from parasympathetic nerves via the activation of 5-HT4 receptors, followed by activation of the nicotinic anti-inflammatory system. It appears that the α7nAChR may be involved in the antiulcerogenic action of mosapride.