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01-03-2012 | Original Article

Differential responses of fibroblasts, non-neoplastic epithelial cells, and oral carcinoma cells to low-level laser therapy

Authors: Volker Hans Schartinger, Oliver Galvan, Herbert Riechelmann, József Dudás

Published in: Supportive Care in Cancer | Issue 3/2012

Abstract

Low-level laser therapy (LLLT) is used in the treatment of chemoradiotherapy- or radiotherapy-induced oropharyngeal mucositis (ORM). In head and neck cancer, tumor cells may lie in the LLLT irradiation field, and LLLT might promote tumor progression. We therefore investigated the effect of LLLT on proliferation, cell cycle distribution, and apoptosis in a human oral carcinoma cell line (SCC-25), non-malignant epithelial cells (BEAS-2B), and fibroblasts in vitro. The cell lines were subjected to LLLT on three consecutive days for 15 min. Cell proliferation was assessed using the MTT assay, cell cycle distribution by flow cytometry and propidium-iodide DNA staining, and apoptosis using an Annexin V-FITC assay. Controls were sham-treated, but not exposed to the laser treatment. LLLT treatment resulted in increased fibroblast proliferation (p < 0.001), whereas decreased cell proliferation was observed after LLLT treatment of BEAS-2B (p = 0.003) and SCC-25 cells (p < 0.001). In SCC-25 cells, an increased percentage of S-phase cells and decreased percentage of G1-phase cells were observed (p < 0.001). Moreover, a proapoptotic effect of LLLT was observed in SCC-25 cells (p = 0.02). LLLT did not exhibit a tumor-promoting effect in this in vitro study.

Treister N, Sonis S (2007) Mucositis: biology and management. Curr Opin Otolaryngol Head Neck Surg 15(2):123–129PubMedCrossRef

Russo G, Haddad R, Posner M, Machtay M (2008) Radiation treatment breaks and ulcerative mucositis in head and neck cancer. Oncologist 13(8):886–898PubMedCrossRef

Rosenthal DI, Trotti A (2009) Strategies for managing radiation-induced mucositis in head and neck cancer. Semin Radiat Oncol 19(1):29–34PubMedCrossRef

Sonis ST (2004) The pathobiology of mucositis. Nat Rev Cancer 4(4):277–284PubMedCrossRef

Reiter R, Deutschle T, Wiegel T, Riechelmann H, Bartkowiak D (2009) Absence of inflammatory response from upper airway epithelial cells after X irradiation. Radiat Res 171(3):274–282PubMedCrossRef

Huang YY, Chen AC, Carroll JD, Hamblin MR (2009) Biphasic dose response in low level light therapy. Dose Response 7(4):358–383PubMedCrossRef

Lopes NN, Plapler H, Chavantes MC, Lalla RV, Yoshimura EM, Alves MT (2009) Cyclooxygenase-2 and vascular endothelial growth factor expression in 5-fluorouracil-induced oral mucositis in hamsters: evaluation of two low-intensity laser protocols. Support Care Canc 17(11):1409–1415CrossRef

Lopes NN, Plapler H, Lalla RV, Chavantes MC, Yoshimura EM, da Silva MA et al (2010) Effects of low-level laser therapy on collagen expression and neutrophil infiltrate in 5-fluorouracil-induced oral mucositis in hamsters. Lasers Surg Med 42(6):546–552PubMedCrossRef

Bensadoun RJ, Franquin JC, Ciais G, Darcourt V, Schubert MM, Viot M et al (1999) Low-energy He/Ne laser in the prevention of radiation-induced mucositis. A multicenter phase III randomized study in patients with head and neck cancer. Support Care Canc 7(4):244–252CrossRef

10.

Genot-Klastersky MT, Klastersky J, Awada F, Awada A, Crombez P, Martinez MD et al (2008) The use of low-energy laser (LEL) for the prevention of chemotherapy- and/or radiotherapy-induced oral mucositis in cancer patients: results from two prospective studies. Support Care Canc 16(12):1381–1387CrossRef

11.

Schubert MM, Eduardo FP, Guthrie KA, Franquin JC, Bensadoun RJ, Migliorati CA et al (2007) A phase III randomized double-blind placebo-controlled clinical trial to determine the efficacy of low level laser therapy for the prevention of oral mucositis in patients undergoing hematopoietic cell transplantation. Support Care Canc 15(10):1145–1154CrossRef

12.

Gao X, Xing D (2009) Molecular mechanisms of cell proliferation induced by low power laser irradiation. J Biomed Sci 16:4PubMedCrossRef

13.

Shefer G, Partridge TA, Heslop L, Gross JG, Oron U, Halevy O (2002) Low-energy laser irradiation promotes the survival and cell cycle entry of skeletal muscle satellite cells. J Cell Sci 115(Pt 7):1461–1469PubMed

14.

Wu S, Xing D, Gao X, Chen WR (2009) High fluence low-power laser irradiation induces mitochondrial permeability transition mediated by reactive oxygen species. J Cell Physiol 218(3):603–611PubMedCrossRef

15.

Pasquali D, Vassallo P, Esposito D, Bonavolonta G, Bellastella A, Sinisi AA (2000) Somatostatin receptor gene expression and inhibitory effects of octreotide on primary cultures of orbital fibroblasts from graves' ophthalmopathy. J Mol Endocrinol 25(1):63–71PubMedCrossRef

16.

Pasquali D, Rossi V, Conzo G, Pannone G, Bufo P, De Bellis A et al (2008) Effects of somatostatin analog SOM230 on cell proliferation, apoptosis, and catecholamine levels in cultured pheochromocytoma cells. J Mol Endocrinol 40(6):263–271PubMed

17.

Aprigliano I, Dudas J, Ramadori G, Saile B (2008) Atorvastatin induces apoptosis by a caspase-9-dependent pathway: an in vitro study on activated rat hepatic stellate cells. Liver Int 28(4):546–557PubMedCrossRef

18.

Genot MT, Klastersky J (2005) Low-level laser for prevention and therapy of oral mucositis induced by chemotherapy or radiotherapy. Curr Opin Oncol 17(3):236–240PubMedCrossRef

19.

Zanin T, Zanin F, Carvalhosa AA, Castro PH, Pacheco MT, Zanin IC et al (2010) Use of 660-nm diode laser in the prevention and treatment of human oral mucositis induced by radiotherapy and chemotherapy. Photomed Laser Surg 28(2):233–237PubMedCrossRef

20.

Kreisler M, Christoffers AB, Al Haj H, Willershausen B, D'Hoedt B (2002) Low level 809-nm diode laser-induced in vitro stimulation of the proliferation of human gingival fibroblasts. Lasers Surg Med 30(5):365–369PubMedCrossRef

21.

Vinck EM, Cagnie BJ, Cornelissen MJ, Declercq HA, Cambier DC (2003) Increased fibroblast proliferation induced by light emitting diode and low power laser irradiation. Lasers Med Sci 18(2):95–99PubMedCrossRef

22.

Pereira AN, Eduardo CP, Matson E, Marques MM (2002) Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts. Lasers Surg Med 31(4):263–267PubMedCrossRef

23.

Pinheiro AL, Carneiro NS, Vieira AL, Brugnera A Jr, Zanin FA, Barros RA et al (2002) Effects of low-level laser therapy on malignant cells: in vitro study. J Clin Laser Med Surg 20(1):23–26PubMedCrossRef

24.

De Castro JL, Pinheiro AL, Werneck CE, Soares CP (2005) The effect of laser therapy on the proliferation of oral KB carcinoma cells: an in vitro study. Photomed Laser Surg 23(6):586–589PubMedCrossRef

25.

Mognato M, Squizzato F, Facchin F, Zaghetto L, Corti L (2004) Cell growth modulation of human cells irradiated in vitro with low-level laser therapy. Photomed Laser Surg 22(6):523–526PubMedCrossRef

26.

Kreisler M, Christoffers AB, Willershausen B, D'Hoedt B (2003) Low-level 809 nm GaAlAs laser irradiation increases the proliferation rate of human laryngeal carcinoma cells in vitro. Lasers Med Sci 18(2):100–103PubMedCrossRef

27.

Pawlik TM, Keyomarsi K (2004) Role of cell cycle in mediating sensitivity to radiotherapy. Int J Radiat Oncol Biol Phys 59(4):928–942PubMedCrossRef

28.

Kujawa J, Zavodnik IB, Lapshina A, Labieniec M, Bryszewska M (2004) Cell survival, DNA, and protein damage in B14 cells under low-intensity near-infrared (810 nm) laser irradiation. Photomed Laser Surg 22(6):504–508PubMedCrossRef

29.

Andree HA, Reutelingsperger CP, Hauptmann R, Hemker HC, Hermens WT, Willems GM (1990) Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers. J Biol Chem 265(9):4923–4928PubMed

30.

Fadok VA, Savill JS, Haslett C, Bratton DL, Doherty DE, Campbell PA et al (1992) Different populations of macrophages use either the vitronectin receptor or the phosphatidylserine receptor to recognize and remove apoptotic cells. J Immunol 149(12):4029–4035PubMed

31.

Bedard K, Krause KH (2007) The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology. Physiol Rev 87(1):245–313PubMedCrossRef

Title: Differential responses of fibroblasts, non-neoplastic epithelial cells, and oral carcinoma cells to low-level laser therapy
Authors: Volker Hans Schartinger
Oliver Galvan
Herbert Riechelmann
József Dudás
Publication date: 01-03-2012
Publisher: Springer-Verlag
Published in: Supportive Care in Cancer / Issue 3/2012
Print ISSN: 0941-4355
Electronic ISSN: 1433-7339
DOI: https://doi.org/10.1007/s00520-011-1113-0

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