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01-06-2006 | Original Article

Kidney NGAL is a novel early marker of acute injury following transplantation

Authors: Jaya Mishra, Qing Ma, Caitlin Kelly, Mark Mitsnefes, Kiyoshi Mori, Jonathan Barasch, Prasad Devarajan

Published in: Pediatric Nephrology | Issue 6/2006

Abstract

Acute kidney injury secondary to ischemia–reperfusion in renal allografts often results in delayed graft function. We tested the hypothesis that expression of neutrophil gelatinase-associated lipocalin (NGAL) is an early marker of acute kidney injury following transplantation. Sections from paraffin-embedded protocol biopsy specimens obtained at approximately one hour of reperfusion after transplantation of 13 cadaveric (CAD) and 12 living-related (LRD) renal allografts were examined by immunohistochemistry for expression of NGAL. The staining intensity was correlated with cold ischemia time, peak post-operative serum creatinine, and dialysis requirement. There were no differences between the LRD and CAD groups in age, gender or preoperative serum creatinine. Using a scoring system of 0 (no staining) to 3 (most intense staining), NGAL expression was significantly increased in CAD specimens (2.3±0.8 versus 0.8±0.7 in LRD, p<0.001). There was a strong correlation between NGAL staining intensity and cold ischemia time (R=0.87, p<0.001). Importantly, NGAL staining in these early CAD biopsies was strongly correlated with peak postoperative serum creatinine, which occurred days later (R=0.86, p<0.001). Four patients developed delayed graft function requiring dialysis during the first week posttransplantation; all of these patients displayed the most intense NGAL staining in their first protocol biopsies. We conclude that NGAL staining intensity in early protocol biopsies represents a novel predictive biomarker of acute kidney injury following transplantation.

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Title: Kidney NGAL is a novel early marker of acute injury following transplantation
Authors: Jaya Mishra
Qing Ma
Caitlin Kelly
Mark Mitsnefes
Kiyoshi Mori
Jonathan Barasch
Prasad Devarajan
Publication date: 01-06-2006
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Nephrology / Issue 6/2006
Print ISSN: 0931-041X
Electronic ISSN: 1432-198X
DOI: https://doi.org/10.1007/s00467-006-0055-0

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Kidney NGAL is a novel early marker of acute injury following transplantation

Abstract

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Abstract

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