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Surgical Endoscopy

Published in:

01-03-2009

Mesna protects splanchnic organs from oxidative stress induced by pneumoperitoneum

Authors: Petros Ypsilantis, Ioannis Tentes, Konstantinos Anagnostopoulos, Alexandros Kortsaris, Constantinos Simopoulos

Published in: Surgical Endoscopy | Issue 3/2009

Abstract

Background

We investigated the potential beneficial effect of the antioxidant 2-mercaptoethane-sulfonate (mesna) against oxidative stress induced by pneumoperitoneum in splanchnic organs.

Methods

Wistar rats were subjected to either (a) CO₂ pneumoperitoneum (15 mmHg for 60 min) (group P), (b) pretreatment with mesna (400 mg/kg, p.o.) followed by pneumoperitoneum with a 180 min interval (group MP), (c) sham operation (group S), or (d) administration of mesna only (group M). Forty-five minutes after desufflation (groups P and MP), 60 + 45 min after the induction of anesthesia (group S), or 180 min after mesna administration (group M), tissue specimens were excised from liver, kidneys, jejunum and stomach. Tissue oxidative state was assessed on the basis of glutathione-to-glutathione disulfide ratio, malondialdehyde concentration , and superoxide dismutase activity.

Results

Pneumoperitoneum deteriorated all the oxidative stress markers in the organs studied. Mesna prevented the occurrence of oxidative stress following pneumoperitoneum in all the organs studied. In the absence of pneumoperitoneum, the administration of mesna caused mild enhancement of the oxidative state of liver, stomach, and kidneys compared to sham controls.

Conclusions

Prophylaxis with mesna prevents oxidative stress induced by pneumoperitoneum in splanchnic organs.

Eleftheriadis E, Kotzampassi K, Papanotas K, Heliadis N, Sarris K (1996) Gut ischemia, oxidative stress, and bacterial translocation in elevated abdominal pressure in rats. World J Surg 20:11–16PubMedCrossRef

Pross M, Schulz HU, Flechsig A, Manger T, Halangk W, Augustin W, Lippert H, Reinheckel T (2000) Oxidative stress in lung tissue induced by CO(2) pneumoperitoneum in the rat. Surg Endosc 14:1180–1184PubMedCrossRef

de Souza AM, Wang CC, Chu CY, Lam PM, Rogers MS (2003) The effect of intra-abdominal pressure on the generation of 8-iso prostaglandin F2alpha during laparoscopy in rabbits. Hum Reprod 18:2181–2188PubMedCrossRef

Yilmaz S, Polat C, Kahraman A, Koken T, Arikan Y, Dilek ON, Gokce O (2004) The comparison of the oxidative stress effects of different gases and intra-abdominal pressures in an experimental rat model. J Laparoendosc Adv Surg Tech A 14:165–168PubMedCrossRef

Glantzounis GK, Tselepis AD, Tambaki AP, Trikalinos TA, Manataki AD, Galaris DA, Tsimoyiannis EC, Kappas AM (2001) Laparoscopic surgery-induced changes in oxidative stress markers in human plasma. Surg Endosc 15:1315–1319PubMedCrossRef

Polat C, Kahraman A, Yilmaz S, Koken T, Serteser M, Akbulut G, Arikan Y, Dilek ON, Gokce O (2003) A comparison of the oxidative stress response and antioxidant capacity of open and laparoscopic hernia repairs. J Laparoendosc Adv Surg Tech A 13:167–173PubMedCrossRef

Ishizaki Y, Bandai Y, Shimomura K, Abe H, Ohtomo Y, Idezuki Y (1993) Changes in splanchnic blood flow and cardiovascular effects following peritoneal insufflation of carbon dioxide. Surg Endosc 7:420–423PubMedCrossRef

Chiu AW, Chang LS, Birkett DH, Babayan RK (1995) The impact of pneumoperitoneum, pneumoretroperitoneum, and gasless laparoscopy on the systemic and renal hemodynamics. J Am Coll Surg 181:397–406PubMed

McDougall EM, Bennett HF, Monk TG, Siegel CL, Li D, McFarland EG, Clayman RV, Sharp T, Rayala HJ, Miller SB, Haacke EM (1997) Functional MR imaging of the porcine kidney: physiologic changes of prolonged pneumoperitoneum. JSLS 1:29–35PubMed

10.

Schafer M, Sagesser H, Reichen J, Krahenbuhl L (2001) Alterations in hemodynamics and hepatic and splachnic circulation during laparoscopy in rats. Surg Endosc 15:1197–1201PubMedCrossRef

11.

Yilmaz S, Koken T, Tokyol C, Kahraman A, Akbulut G, Serteser M, Polat C, Gokce C, Gokce O (2003) Can preconditioning reduce laparoscopy-induced tissue injury? Surg Endosc 17:819–824PubMedCrossRef

12.

Yilmaz S, Ates E, Polat C, Koken T, Tokyol C, Akbulut G, Gokce O (2003) Ischemic preconditioning decreases laparoscopy-induced oxidative stress in small intestine. Hepatogastroenterology 50:979–982PubMed

13.

Cevrioglu AS, Yilmaz S, Koken T, Tokyol C, Yilmazer M, Fenkci IV (2004) Comparison of the effects of low intra-abdominal pressure and ischaemic preconditioning on the generation of oxidative stress markers and inflammatory cytokines during laparoscopy in rats. Hum Reprod 19:2144–2151PubMedCrossRef

14.

Ates E, Yilmaz S, Ihtiyar E, Yasar B, Karahuseyinoglu E (2006) Preconditioning-like amelioration of erythropoietin against laparoscopy-induced oxidative injury. Surg Endosc 20:815–819PubMedCrossRef

15.

Cay A, Imamoglu M, Unsal MA, Aydin S, Alver A, Akyol A, Sarihan H (2006) Does anti-oxidant prophylaxis with melatonin prevent adverse outcomes related to increased oxidative stress caused by laparoscopy in experimental rat model? J Surg Res 135:2–8PubMedCrossRef

16.

Dechant KL, Brogden RN, Pilkington T, Faulds D, Ifosfamide/mesna (1991) A review of its antineoplastic activity, pharmacokinetic properties and therapeutic efficacy in cancer. Drugs 42:428–467

17.

Haselberger MB, Schwinghammer TL (1995) Efficacy of mesna for prevention of hemorrhagic cystitis after high-dose cyclophosphamide therapy. Ann Pharmacother 29:918–921PubMed

18.

Siu LL, Moore MJ (1998) Use of mesna to prevent ifosfamide-induced urotoxicity. Support Care Cancer 6:144–154PubMedCrossRef

19.

Kabasakal L, Sehirli AO, Cetinel S, Cikler E, Gedik N, Sener G (2004) Mesna (2-mercaptoethane sulfonate) prevents ischemia/reperfusion induced renal oxidative damage in rats. Life Sci 75:2329–2340PubMedCrossRef

20.

Sener G, Sehirli O, Ercan F, Sirvanci S, Gedik N, Kacmaz A (2005) Protective effect of MESNA (2-mercaptoethane sulfonate) against hepatic ischemia/reperfusion injury in rats. Surg Today 35:575–580PubMedCrossRef

21.

Ypsilantis P, Lambropoulou M, Tentes I, Kortsaris A, Papadopoulos N, Simopoulos C (2006) Mesna protects intestinal mucosa from ischemia-reperfusion injury. J Surg Res 134:278–284PubMedCrossRef

22.

Ypsilantis P, Tentes I, Lambropoulou M, Anagnostopoulos K, Papadopoulos N, Kortsaris A, Simopoulos C (2008) Prophylaxis with mesna prevents oxidative stress induced by ischemia-reperfusion in the intestine via inhibition of nuclear factor-κB activation. J Gastroen Hepatol 23:328–335

23.

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254PubMedCrossRef

24.

Bearden JC Jr (1978) Quantitation of submicrogram quantities of protein by an improved protein-dye binding assay. Biochim Biophys Acta 533:525–529PubMed

25.

Toyokuni S (1999) Reactive oxygen species-induced molecular damage and its application in pathology. Pathol Int 49:91–102PubMedCrossRef

26.

Brivida K, Sies H (1994) Non enzymatic antioxidant defense system. In: Frei B (ed) Natural Antioxidants in Human Health and Disease. Academic Press, San Diego, pp 107–128

27.

de Zwart LL, Meerman JH, Commandeur JN, Vermeulen NP (1999) Biomarkers of free radical damage applications in experimental animals and in humans. Free Radic Biol Med 26:202–226PubMedCrossRef

28.

Carden DL, Granger DN (2000) Pathophysiology of ischaemia-reperfusion injury. J Pathol 190:255–266PubMedCrossRef

29.

Granger DN, Korthuis RJ (1995) Physiologic mechanisms of postischemic tissue injury. Annu Rev Physiol 57:311–332PubMed

30.

Windberger UB, Auer R, Keplinger F, Längle F, Heinze G, Schindl M, Losert UM (1999) The role of intra-abdominal pressure on splanchnic and pulmonary hemodynamic and metabolic changes during carbon dioxide pneumoperitoneum. Gastrointest Endosc 49:84–91PubMedCrossRef

31.

Stofer-Vogel B, Cerny T, Borner M, Lauterburg BH (1993) Oral bioavailability of mesna tablets. Cancer Chemother. Pharmacol 32:78–81

32.

Gressier B, Cabanis A, Lebegue S, Brunet C, Dine T, Luyckx M, Cazin M, Cazin JC (1994) Decrease of hypochlorous acid and hydroxyl radical generated by stimulated human neutrophils: comparison in vitro of some thiol-containing drugs. Methods Find Exp Clin Pharmacol 16:9–13PubMed

33.

Pahl HL (1999) Activators and target genes of Rel/NF-κB transcription factors. Oncogene 18:6853–6866PubMedCrossRef

34.

Gloire G, Legrand-Poels S, Piette J (2006) NF-kappa B activation by reactive oxygen species: fifteen years later. Biochem Pharmacol 72:1493–1505PubMedCrossRef

35.

Schulze-Osthoff K, Beyaert R, Vandevoorde V, Haegeman G, Fiers W (1993) Depletion of the mitochondrial electron transport abrogates the cytotoxic and gene-inductive effects of TNF. EMBO J 12:3095–3104PubMed

36.

Sen CK, Roy S, Packer L (1996) Involvement of intracellular Ca2+ in oxidant-induced NF-kappa B activation. FEBS Lett 385:58–62PubMedCrossRef

37.

Manna SK, Kuo MT, Aggarwal BB (1999) Overexpression of gamma-glutamylcysteine synthetase suppresses tumor necrosis factor-induced apoptosis and activation of nuclear transcription factor-kappa B and activator protein-1. Oncogene 18:4371–4382PubMedCrossRef

Title: Mesna protects splanchnic organs from oxidative stress induced by pneumoperitoneum
Authors: Petros Ypsilantis
Ioannis Tentes
Konstantinos Anagnostopoulos
Alexandros Kortsaris
Constantinos Simopoulos
Publication date: 01-03-2009
Publisher: Springer-Verlag
Published in: Surgical Endoscopy / Issue 3/2009
Print ISSN: 0930-2794
Electronic ISSN: 1432-2218
DOI: https://doi.org/10.1007/s00464-008-9887-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Mesna protects splanchnic organs from oxidative stress induced by pneumoperitoneum

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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