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Surgical Endoscopy

Published in:

01-10-2006

Impact of carbon dioxide versus air pneumoperitoneum on peritoneal cell migration and cell fate

Authors: U. Moehrlen, U. Ziegler, E. Boneberg, E. Reichmann, C. A. Gitzelmann, M. Meuli, J. Hamacher

Published in: Surgical Endoscopy | Issue 10/2006

Abstract

Background

Postoperative systemic immune function is suppressed after open abdominal surgery, as compared with that after minimally invasive abdominal surgery. As a first line of defense, peritoneal macrophages (PMo) and polymorphonuclear neutrophil granulocytes (PMNs) are of primary importance in protecting the body from microorganisms. Previous studies have shown changes in these cell populations over time after open versus laparoscopic surgery. This study aimed to investigate the dynamics of cell recruitment and clearance of peritoneal cells.

Methods

Female NMRI mice (33 ± 2 g) were randomly assigned to carbon dioxide (CO₂) or air insufflation. Intravasal cells with phagocytic capabilities were selectively stained by intravenous injection of the fluorescent dye PKH26 24 h before surgery. Gas was insufflated into the peritoneal cavity through a catheter, and the pneumoperitoneum was maintained for 30 min. Peritoneal lavage was performed 1, 3, 8, or 24 h after surgery. Apoptotic cells were assessed by flow cytometry using a general caspase substrate.

Results

The total peritoneal cell count did not differ between groups. The PKH26-positive PMo level was significantly increased after CO₂, as compared with air, at 1 h and 24 h. The ratio of apoptotic PMo did not differ between the groups. In the peritoneal lavage, polymorphonuclear leukocytes (PMNs) were tripled in the air group, as compared with the CO₂ group, whereas the ratio of apoptotic PMNs was significantly decreased. There was a higher fraction of PKH26-positive PMNs after air exposure, as compared with that after CO₂.

Conclusions

Air exposure triggered a higher transmigration rate of PMNs from the blood compartment into the peritoneal cavity and decreased PMN apoptosis, as compared with CO₂. The lower proportion of PKH26-positive peritoneal macrophages in the air group might have been attributable to a higher inflammatory stimulation than in the CO₂ group, leading to increased emigration of PMo to draining lymph nodes. All the findings underscore a complex cell-specific regulation of cell recruitment and clearance in the peritoneal compartment.

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Title: Impact of carbon dioxide versus air pneumoperitoneum on peritoneal cell migration and cell fate
Authors: U. Moehrlen
U. Ziegler
E. Boneberg
E. Reichmann
C. A. Gitzelmann
M. Meuli
J. Hamacher
Publication date: 01-10-2006
Publisher: Springer-Verlag
Published in: Surgical Endoscopy / Issue 10/2006
Print ISSN: 0930-2794
Electronic ISSN: 1432-2218
DOI: https://doi.org/10.1007/s00464-005-0775-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Impact of carbon dioxide versus air pneumoperitoneum on peritoneal cell migration and cell fate

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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