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Journal of Cancer Research and Clinical Oncology

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01-05-2016 | Original Article – Cancer Research

Expression of the apoptosis repressor with caspase recruitment domain (ARC) in liver metastasis of colorectal cancer and its correlation with DNA mismatch repair proteins and p53

Authors: Csaba Tóth, Jeannine Meinrath, Esther Herpel, Jutta Derix, Jochen Fries, Reinhard Buettner, Peter Schirmacher, Sebastian Heikaus

Published in: Journal of Cancer Research and Clinical Oncology | Issue 5/2016

Abstract

Introduction

Apoptotic signaling is one of the most important processes in the measurement of chemotherapeutic effectiveness. In apoptotic machinery, various pathways and proteins are involved (i.e., mismatch repair proteins, p53). One of the regulatory proteins is ARC, which can inhibit not only the extrinsic but also the intrinsic apoptotic signaling.

Materials and Methods

In this study, we investigated the expression levels of ARC in colorectal liver metastasis and compared them with the expression of mismatch repair proteins and p53. Furthermore, we investigated ARC expression level depending on sex, age, tumor grade, mucin production, tumor size and number of liver metastasis.

Results

ARC expression level in colorectal cancer liver metastasis was independent from clinical data (i.e., age, gender, tumor size, tumor number or mucin production) but strongly correlated with MSH2 and MSH6 expression, which further supported the evidence for the regulatory role of MSH2 and MSH6 in apoptosis; i.e., in case of sufficient MSH2 and MSH6 expression, significantly higher ARC level is required to suppress the apoptosis. A regulatory interaction between ARC and p53 has been described, but we found no correlation between p53 expression levels and ARC levels.

Conclusion

Further studies are needed to define the exact role of ARC in apoptotic signaling and thus its role in chemoresistance and survival of tumor cells.

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Title: Expression of the apoptosis repressor with caspase recruitment domain (ARC) in liver metastasis of colorectal cancer and its correlation with DNA mismatch repair proteins and p53
Authors: Csaba Tóth
Jeannine Meinrath
Esther Herpel
Jutta Derix
Jochen Fries
Reinhard Buettner
Peter Schirmacher
Sebastian Heikaus
Publication date: 01-05-2016
Publisher: Springer Berlin Heidelberg
Published in: Journal of Cancer Research and Clinical Oncology / Issue 5/2016
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-015-2102-3

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Abstract

Introduction

Materials and Methods

Results

Conclusion

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