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01-09-2009 | Original Paper

Increased nitric oxide production by neutrophils in early stage of Kawasaki disease

Authors: Ken Yoshimura, Kimiko Tatsumi, Anna Iharada, Shoji Tsuji, Ai Tateiwa, Masayuki Teraguchi, Hirotaro Ogino, Kazunari Kaneko

Published in: European Journal of Pediatrics | Issue 9/2009

Abstract

Recent observations suggested that nitric oxide (NO) has a role in triggering the early endothelial dysfunction in Kawasaki disease (KD). We investigated the amount of NO in conjunction with reactive oxygen species (ROS) produced by neutrophils in children with acute KD by a newly developed flow cytometric analysis. Forty children with acute KD (n = 14), non-KD febrile disease (n = 14), and afebrile control (n = 12) were enrolled (age, 3 to 88 months). Neutrophils in KD produced significantly higher amount of NO compared to others (p < 0.05). With regard to ROS, significant increase was not only found in KD but also in non-KD febrile children (p < 0.05 and p < 0.01, respectively). In KD patients, the amount of NO produced by neutrophils decreased after immunoglobulin (IVIG) treatment, while there was no significant change in ROS production. The amount of NO in KD patients also correlated well with the days from the onset. One patient who developed coronary arterial lesion showed the highest value of NO. In conclusion, neutrophils in acute KD generate both NO and ROS considerably, while NO production is exclusive in the early stage of KD before IVIG treatment. Abnormal immune system in KD might be characterized by an overproduction of NO, whereas the role of NO in endothelial damage remains to be elucidated.

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Title: Increased nitric oxide production by neutrophils in early stage of Kawasaki disease
Authors: Ken Yoshimura
Kimiko Tatsumi
Anna Iharada
Shoji Tsuji
Ai Tateiwa
Masayuki Teraguchi
Hirotaro Ogino
Kazunari Kaneko
Publication date: 01-09-2009
Publisher: Springer-Verlag
Published in: European Journal of Pediatrics / Issue 9/2009
Print ISSN: 0340-6199
Electronic ISSN: 1432-1076
DOI: https://doi.org/10.1007/s00431-008-0872-1

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Increased nitric oxide production by neutrophils in early stage of Kawasaki disease

Abstract

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Abstract

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