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01-09-2013 | Original Article

Stage-dependent detection of CD14⁺ and CD16⁺ cells in the human heart after myocardial infarction

Authors: Frauke S. Czepluch, Magdalena Schlegel, Felix Bremmer, Carl L. Behnes, Gerd Hasenfuss, Katrin Schäfer

Published in: Virchows Archiv | Issue 3/2013

Abstract

Monocytes are critically involved in cardiovascular wound healing processes. Human monocytes can be classified into two subsets based on the expression of CD14 and CD16. Here, we examined the temporal and spatial distribution of CD14⁺ and CD16⁺ cells after myocardial infarction (MI) in human heart and spleen tissue and correlated it with markers of cardiac repair. Heart samples obtained at autopsy were histologically classified into acute (AMI; n = 11), subacute (SAMI; n = 10) and old (OMI; n = 16) MI, or control myocardium (CONTR; n = 8). Histochemical analyses revealed marked fibrosis in OMI (p < 0.001 vs. CONTR). The adhesion molecule CD56 was also strongly expressed in OMI (p < 0.01 vs. CONTR) and found to correlate with fibrosis (p < 0.001). The number of capillaries was reduced in OMI (p < 0.01 vs. CONTR; p < 0.05 vs. AMI), whereas the hypoxia indicator carbonic anhydrase IX was predominantly expressed in AMI (p < 0.01 vs. OMI and CONTR) and SAMI (p < 0.05 vs. OMI and CONTR). The monocyte chemoattractrant osteopontin was also more highly expressed in hearts of SAMI patients (p < 0.01 vs. CONTR). Numbers of CD14⁺ monocytes were found to correlate with CD16⁺ cells (p < 0.05) and inversely with fibrosis (p < 0.05). Regarding a MI-associated release of monocytes from spleen reservoirs, a non-significant reduction of splenic CD14⁺ and CD16⁺ cells was detected in subjects with AMI. In conclusion, disease stage-specific alterations in CD14⁺ and CD16⁺ cells in human heart may contribute to cardiac repair processes following MI.

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Title: Stage-dependent detection of CD14+ and CD16+ cells in the human heart after myocardial infarction
Authors: Frauke S. Czepluch
Magdalena Schlegel
Felix Bremmer
Carl L. Behnes
Gerd Hasenfuss
Katrin Schäfer
Publication date: 01-09-2013
Publisher: Springer Berlin Heidelberg
Published in: Virchows Archiv / Issue 3/2013
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-013-1447-8

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Stage-dependent detection of CD14⁺ and CD16⁺ cells in the human heart after myocardial infarction

Abstract

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Abstract

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