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01-04-2013 | Original Article

Detection of HPV infection in head and neck squamous cell carcinoma: a practical proposal

Authors: Johannes H. Dreyer, Franziska Hauck, Michelle Oliveira-Silva, Mario Henrique M. Barros, Gerald Niedobitek

Published in: Virchows Archiv | Issue 4/2013

Abstract

Detecting human papillomavirus (HPV) infection in head and neck squamous cell carcinoma (HNSCC) is clinically relevant, but there is no agreement about the most appropriate methodology. We have studied 64 oropharyngeal carcinomas using p16 immunohistochemistry, HPV DNA in situ hybridisation (ISH) and HPV DNA polymerase chain reaction (PCR) followed by pyrosequencing. We have also evaluated a new assay, RNAscope, designed to detect HPV E6/E7 RNA transcripts. Using a threshold of 70 % labelled tumour cells, 21 cases (32.8 %) were p16 positive. Of these, 19 cases scored positive with at least one HPV detection assay. Sixteen cases were positive by HPV DNA-ISH, and 18 cases were positive using the E6/E7 RNAscope assay. By PCR and pyrosequencing, HPV16 was detected in 15 cases, while one case each harboured HPV33, 35 and 56. All p16-negative cases were negative using these assays. We conclude that p16 expression is a useful surrogate marker for HPV infection in HNSCC with a high negative predictive value and that p16-positive cases should be further evaluated for HPV infection, preferably by PCR followed by type determination. Using RNase digestion experiments, we show that the RNAscope assay is not suitable for the reliable discrimination between E6/E7 RNA transcripts and viral DNA.

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Title: Detection of HPV infection in head and neck squamous cell carcinoma: a practical proposal
Authors: Johannes H. Dreyer
Franziska Hauck
Michelle Oliveira-Silva
Mario Henrique M. Barros
Gerald Niedobitek
Publication date: 01-04-2013
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 4/2013
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-013-1393-5

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Detection of HPV infection in head and neck squamous cell carcinoma: a practical proposal

Abstract

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