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01-12-2007 | Original Article

Ezrin and alpha-smooth muscle actin are immunohistochemical prognostic markers in conventional osteosarcomas

Authors: Sébastien Salas, Catherine Bartoli, Jean-Laurent Deville, Jean Gaudart, Fréderic Fina, Arlette Calisti, Gérard Bollini, Georges Curvale, Jean-Claude Gentet, Florence Duffaud, Dominique Figarella-Branger, Corinne Bouvier

Published in: Virchows Archiv | Issue 6/2007

Abstract

Ezrin is a cytoskeleton linker protein that is actively involved in the metastatic process of cancer cells. We have searched for a prognostic value of ezrin and some of its partners: α-smooth muscle actin and CD44H in 37 patients with an osteosarcoma. Automate immunohistochemistry (IHC) with anti-ezrin, α-smooth muscle actin and CD44H antibodies was performed in 66 specimens: 37 biopsies before chemotherapy, 16 resected tumours of “poor” responders and 13 metastases. The messenger RNA (mRNA) levels of ezrin of 13 frozen biopsies and 4 metastases were evaluated by real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR). All results were correlated to the following clinical data. Ezrin expression by IHC was found in 62% of 37 biopsies in the different histological subtypes. A good correlation was found between positive or negative samples by IHC and mRNA levels. Ezrin expression was recorded in 84.5% of metastastic samples. The mean expression of ezrin was higher in metastases than biopsies (p = 0.024). In multivariate analysis, ezrin was an independent prognostic marker for event-free survival and overall survival (OS) with p < 0.001 and p = 0.003, respectively, and α-smooth muscle actin for OS only (p = 0.024). Our findings suggest that ezrin and α-smooth muscle actin are predictive IHC prognostic markers for patients with an osteosarcoma.

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Title: Ezrin and alpha-smooth muscle actin are immunohistochemical prognostic markers in conventional osteosarcomas
Authors: Sébastien Salas
Catherine Bartoli
Jean-Laurent Deville
Jean Gaudart
Fréderic Fina
Arlette Calisti
Gérard Bollini
Georges Curvale
Jean-Claude Gentet
Florence Duffaud
Dominique Figarella-Branger
Corinne Bouvier
Publication date: 01-12-2007
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 6/2007
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-007-0474-8

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Ezrin and alpha-smooth muscle actin are immunohistochemical prognostic markers in conventional osteosarcomas

Abstract

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