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01-09-2005 | Original Article

Tracing origin of serrated adenomas with BRAF and KRAS mutations

Authors: Eui Jin Lee, Chan Choi, Cheol Keun Park, Leeso Maeng, Jehoon Lee, Anhi Lee, Kyoung-Mee Kim

Published in: Virchows Archiv | Issue 3/2005

Abstract

Serrated neoplasm of the colorectum raised many as-yet unanswered issues. To characterize serrated neoplasia pathway, we investigated BRAF and KRAS mutations in 35 traditional serrated adenomas. BRAF exons 11 and 15, and KRAS exon 2 were amplified by polymerase chain reaction and directly sequenced. BRAF V599E mutation was found in 27 serrated adenomas (77.1%), and KRAS mutations were found in 3 (8.6%) of 35 traditional serrated adenomas. In 13 cases, mixed polyps composed of traditional serrated adenomas and hyperplastic (serrated) polyps were observed, and seven of them showed the same BRAF mutations in both components. Somatic mutations of BRAF and KRAS genes were mutually exclusive. These findings suggest that BRAF mutations are early and a critical event in the serrated adenomas, and most serrated adenomas in both sides of colon may progress from microvesicular hyperplastic polyps via BRAF mutations, and some left-sided serrated adenomas develop via KRAS mutations.

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Title: Tracing origin of serrated adenomas with BRAF and KRAS mutations
Authors: Eui Jin Lee
Chan Choi
Cheol Keun Park
Leeso Maeng
Jehoon Lee
Anhi Lee
Kyoung-Mee Kim
Publication date: 01-09-2005
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 3/2005
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-005-1226-2

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Tracing origin of serrated adenomas with BRAF and KRAS mutations

Abstract

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