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01-11-2005 | Original Article

Expression of small intestinal and colonic phenotypes in complete intestinal metaplasia of the human stomach

Authors: Harunari Tanaka, Tetsuya Tsukamoto, Tsutomu Mizoshita, Ken-ichi Inada, Naotaka Ogasawara, Xueyuan Cao, Sosuke Kato, Takashi Joh, Masae Tatematsu

Published in: Virchows Archiv | Issue 5/2005

Abstract

The incomplete intestinal metaplasia (IM) that is reported to be a risk factor for gastric carcinogenesis in man usually features sulfomucin production and thus is considered of colonic type. To cast light on the underlying mechanisms, we here examined the proportions of colonic and small intestinal phenotypes in IM by immunohistochemistry and real-time reverse transcription–polymerase chain reaction at the single isolated gland level. Carbonic anhydrase 1 (CA1) is a specific marker of colonic epithelial cells, whereas sucrase is specific to absorptive cells of the small intestine. Totals of 139 (23.5%) and 452 (76.5%) IM glands were judged to be CA1 positive and CA1 negative, respectively, in resected pyloric mucosa from cancer patients. The average score for MUC5AC in CA1-positive IMs was significantly lower than in CA1-negative counterpart tissue (P<0.0001), whereas the opposite was the case for sucrase (P<0.0001). High iron diamine–Alcian blue staining revealed CA1 expression to coincide with type I complete IM. The expression of CA1 mRNA strongly correlated with that of sucrase–isomaltase, and inversely with that of MUC5AC in isolated IM glands. In conclusion, CA1 could be colocalized with small intestinal proteins such as sucrase, but only rarely with the gastric mucin, MUC5AC. Its expression warrants further study, with the focus on stimulation and/or suppression mechanisms by gastric and intestinal transcription factors.

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Title: Expression of small intestinal and colonic phenotypes in complete intestinal metaplasia of the human stomach
Authors: Harunari Tanaka
Tetsuya Tsukamoto
Tsutomu Mizoshita
Ken-ichi Inada
Naotaka Ogasawara
Xueyuan Cao
Sosuke Kato
Takashi Joh
Masae Tatematsu
Publication date: 01-11-2005
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 5/2005
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-005-0040-1

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Expression of small intestinal and colonic phenotypes in complete intestinal metaplasia of the human stomach

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