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01-08-2003 | Original Article

Tissue microarray technology: validation in colorectal carcinoma and analysis of p53, hMLH1, and hMSH2 immunohistochemical expression

Authors: Florence Jourdan, Nicole Sebbagh, Eva Comperat, Najat Mourra, Antoine Flahault, Sylviane Olschwang, Alex Duval, Richard Hamelin, Jean-François Flejou

Published in: Virchows Archiv | Issue 2/2003

Abstract

Tissue microarray technology enables the analysis of hundreds of specimens by arranging numerous 0.6-mm tissue core biopsy specimens into a single paraffin block. Validation studies are necessary to evaluate the representativeness of small disks taken from the original tissue. We validated the tissue microarray technology in colorectal carcinoma by analyzing the immunohistochemical expression of proteins involved in the two main pathways of colorectal carcinogenesis: p53 protein for loss of heterozygosity tumors, hMLH1 and hMSH2 proteins for microsatellite instability (MSI) tumors. We compared in 30 colorectal carcinomas (15 MSI⁻ and 15 MSI⁺), 8 microarrays disks, and the whole section of the block from which they were derived. Tumoral tissue was present in 95.7% of the microarray disks. The analysis of three disks per case was comparable to the analysis of the whole section in 99.6% (p53), 98.8% (hMLH1), and 99.2% (hMSH2) of cases. In the second part we applied the tissue microarray technology to 263 consecutive cases of colorectal carcinoma, sampled by three cores. We showed that 48.5% overexpressed p53 and 8.7% lost hMLH1 or hMSH2. Tissue microarray technology, validated in colorectal carcinoma, appears as a useful research tool for rapid analysis of the clinical interest of molecular alterations.

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Title: Tissue microarray technology: validation in colorectal carcinoma and analysis of p53, hMLH1, and hMSH2 immunohistochemical expression
Authors: Florence Jourdan
Nicole Sebbagh
Eva Comperat
Najat Mourra
Antoine Flahault
Sylviane Olschwang
Alex Duval
Richard Hamelin
Jean-François Flejou
Publication date: 01-08-2003
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 2/2003
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-003-0833-z

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Tissue microarray technology: validation in colorectal carcinoma and analysis of p53, hMLH1, and hMSH2 immunohistochemical expression

Abstract

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Abstract

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