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Published in: European Journal of Applied Physiology 4/2017

Open Access 01-04-2017 | Original Article

Dietary nitrate supplementation enhances short but not longer duration running time-trial performance

Authors: Oliver Michael Shannon, Matthew John Barlow, Lauren Duckworth, Emily Williams, Georgina Wort, David Woods, Mario Siervo, John Paul O’Hara

Published in: European Journal of Applied Physiology | Issue 4/2017

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Abstract

Purpose

This study evaluated the effects of dietary nitrate (NO3 ) supplementation on physiological functioning and exercise performance in trained runners/triathletes conducting short and longer-distance treadmill running time-trials (TT).

Method

Eight trained male runners or triathletes completed four exercise performance tests comprising a 10 min warm up followed by either a 1500 or 10,000 m treadmill TT. Exercise performance tests were preceded 3 h before the exercise by supplementation with either 140 ml concentrated nitrate-rich (~12.5 mmol nitrate) (BRJ) or nitrate-deplete (~0.01 mmol nitrate) (PLA) beetroot juice.

Results

BRJ supplementation significantly elevated plasma [NO2 ] (P < 0.05). Resting blood pressure and exercise \(\dot{\text{V}}{{\text{O}}_{\text{2}}}\) were not significantly different between BRJ and PLA (P > 0.05). However, post-exercise blood [lactate] was significantly greater in BRJ following the 1500 m TT (6.6 ± 1.2 vs. 6.1 ± 1.5 mM; P < 0.05), but not significantly different between conditions in the 10,000 m TT (P > 0.05). Performance in the 1500 m TT was significantly faster in BRJ vs. PLA (319.6 ± 36.2 vs. 325.7 ± 38.8 s; P < 0.05). Conversely, there was no significant difference in 10,000 m TT performance between conditions (2643.1 ± 324. 1 vs. 2649.9 ± 319.8 s, P > 0.05).

Conclusion

Acute BRJ supplementation significantly enhanced 1500 m, but not 10,000 m TT performance. These findings suggest that BRJ might be ergogenic during shorter distance TTs which allow for a high work rate, but not during longer distance TTs, completed at a lower work rate.
Literature
go back to reference Ashworth A, Mitchell K, Blackwell JR, Vanhatalo A, Jones AM (2015) High-nitrate vegetable diet increases plasma nitrate and nitrite concentrations and reduces blood pressure in healthy women. Public Health Nutr 18:2669–2678. doi:10.1017/S1368980015000038 CrossRefPubMed Ashworth A, Mitchell K, Blackwell JR, Vanhatalo A, Jones AM (2015) High-nitrate vegetable diet increases plasma nitrate and nitrite concentrations and reduces blood pressure in healthy women. Public Health Nutr 18:2669–2678. doi:10.​1017/​S136898001500003​8 CrossRefPubMed
go back to reference Bailey SJ, Winyard P, Vanhatalo A, Blackwell JR, DiMenna FJ, Wilkerson DP, Tarr J, Benjamin N, Jones AM (2009) Dietary nitrate supplementation reduces the O2 cost of low-intensity exercise and enhances tolerance to high-intensity exercise in humans. J Appl Physiol 107:1144–1155. doi:10.1152/japplphysiol.00722.2009 CrossRefPubMed Bailey SJ, Winyard P, Vanhatalo A, Blackwell JR, DiMenna FJ, Wilkerson DP, Tarr J, Benjamin N, Jones AM (2009) Dietary nitrate supplementation reduces the O2 cost of low-intensity exercise and enhances tolerance to high-intensity exercise in humans. J Appl Physiol 107:1144–1155. doi:10.​1152/​japplphysiol.​00722.​2009 CrossRefPubMed
go back to reference Bailey SJ, Fulford J, Vanhatalo A, Winyard PG, Blackwell JR, DiMenna FJ, Wilkerson DP, Benjamin N, Jones AM (2010) Dietary nitrate supplementation enhances muscle contractile efficiency during knee-extensor exercise in humans. J Appl Physiol 109:135–148. doi:10.1152/japplphysiol.00046.2010 CrossRefPubMed Bailey SJ, Fulford J, Vanhatalo A, Winyard PG, Blackwell JR, DiMenna FJ, Wilkerson DP, Benjamin N, Jones AM (2010) Dietary nitrate supplementation enhances muscle contractile efficiency during knee-extensor exercise in humans. J Appl Physiol 109:135–148. doi:10.​1152/​japplphysiol.​00046.​2010 CrossRefPubMed
go back to reference Bailey SJ, Varnham RL, DiMenna FJ, Breese BC, Wylie LJ, Jones AM (2015) Inorganic nitrate supplementation improves muscle oxygenation, O2 uptake kinetics, and exercise tolerance at high but not low pedal rates. J Appl Physiol 118:1396–1405. doi:10.1152/japplphysiol.01141.2014 CrossRefPubMed Bailey SJ, Varnham RL, DiMenna FJ, Breese BC, Wylie LJ, Jones AM (2015) Inorganic nitrate supplementation improves muscle oxygenation, O2 uptake kinetics, and exercise tolerance at high but not low pedal rates. J Appl Physiol 118:1396–1405. doi:10.​1152/​japplphysiol.​01141.​2014 CrossRefPubMed
go back to reference Bescós R, Ferrer-Roca V, Galilea PA, Roig A, Drobnic F, Sureda A, Martorell M, Cordova A, Tur JA, Pons A (2012) Sodium nitrate supplementation does not enhance performance of endurance athletes. Med Sci Sports Exerc 44:2400–2409. doi:10.1249/MSS.0b013e3182687e5c CrossRefPubMed Bescós R, Ferrer-Roca V, Galilea PA, Roig A, Drobnic F, Sureda A, Martorell M, Cordova A, Tur JA, Pons A (2012) Sodium nitrate supplementation does not enhance performance of endurance athletes. Med Sci Sports Exerc 44:2400–2409. doi:10.​1249/​MSS.​0b013e3182687e5c​ CrossRefPubMed
go back to reference Breese BC, McNarry MA, Marwood S, Blackwell JR, Bailey SJ, Jones AM (2013) Beetroot juice supplementation speeds O2 uptake kinetics and improves exercise tolerance during severe-intensity exercise initiated from an elevated metabolic rate. Am J Physiol Regul Integr Comp Physiol 305:R1441–R1450. doi:10.1152/ajpregu.00295.2013 CrossRefPubMed Breese BC, McNarry MA, Marwood S, Blackwell JR, Bailey SJ, Jones AM (2013) Beetroot juice supplementation speeds O2 uptake kinetics and improves exercise tolerance during severe-intensity exercise initiated from an elevated metabolic rate. Am J Physiol Regul Integr Comp Physiol 305:R1441–R1450. doi:10.​1152/​ajpregu.​00295.​2013 CrossRefPubMed
go back to reference Castello PR, David PS, McClure T, Crook Z, Poyton RO (2006) Mitochondrial cytochrome oxidase produces nitric oxide under hypoxic conditions: implications for oxygen sensing and hypoxic signalling in eukaryotes. Cell Metab 3:277–287. doi:10.1016/j.cmet.2006.02.011 CrossRefPubMed Castello PR, David PS, McClure T, Crook Z, Poyton RO (2006) Mitochondrial cytochrome oxidase produces nitric oxide under hypoxic conditions: implications for oxygen sensing and hypoxic signalling in eukaryotes. Cell Metab 3:277–287. doi:10.​1016/​j.​cmet.​2006.​02.​011 CrossRefPubMed
go back to reference Cermak NM, Gibala MJ, van Loon LJ, others (2012a) Nitrate supplementation’s improvement of 10-km time-trial performance in trained cyclists. Int J Sport Nutr Exerc Metab 22:64–71CrossRefPubMed Cermak NM, Gibala MJ, van Loon LJ, others (2012a) Nitrate supplementation’s improvement of 10-km time-trial performance in trained cyclists. Int J Sport Nutr Exerc Metab 22:64–71CrossRefPubMed
go back to reference Cermak NM, Res P, Stinkens R, Lundberg JO, Gibala MJ, van Loon L JC (2012b) No improvement in endurance performance after a single dose of beetroot juice. Int J Sport Nutr Exerc Metab 22:470–478CrossRefPubMed Cermak NM, Res P, Stinkens R, Lundberg JO, Gibala MJ, van Loon L JC (2012b) No improvement in endurance performance after a single dose of beetroot juice. Int J Sport Nutr Exerc Metab 22:470–478CrossRefPubMed
go back to reference Coggan AR, Leibowitz JL, Kadkhodayan A, Thomas DP, Ramamurthy S, Spearie CA, Waller S, Farmer M, Peterson LR (2015) Effect of acute dietary nitrate intake on maximal knee extensor speed and power in healthy men and women. Nitric Oxide 48:16–21. doi:10.1016/j.niox.2014.08.014 CrossRefPubMed Coggan AR, Leibowitz JL, Kadkhodayan A, Thomas DP, Ramamurthy S, Spearie CA, Waller S, Farmer M, Peterson LR (2015) Effect of acute dietary nitrate intake on maximal knee extensor speed and power in healthy men and women. Nitric Oxide 48:16–21. doi:10.​1016/​j.​niox.​2014.​08.​014 CrossRefPubMed
go back to reference Cosby K, Partovi KS, Crawford JH, Patel RP, Reiter CD, Martyr S, Yang BK, Waclawiw MA, Zalos G, Xu X, Huang KT, Shields H, Kim-Shapiro DB, Schechter AN, Cannon RO, Gladwin MT (2003) Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation. Nat Med 9:1498–1505. doi:10.1038/nm954 CrossRefPubMed Cosby K, Partovi KS, Crawford JH, Patel RP, Reiter CD, Martyr S, Yang BK, Waclawiw MA, Zalos G, Xu X, Huang KT, Shields H, Kim-Shapiro DB, Schechter AN, Cannon RO, Gladwin MT (2003) Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation. Nat Med 9:1498–1505. doi:10.​1038/​nm954 CrossRefPubMed
go back to reference Duncan C, Dougall H, Johnston P, Green S, Brogan R, Leifert C, Smith L, Golden M, Benjamin N (1995) Chemical generation of nitric oxide in the mouth from the enterosalivary circulation of dietary nitrate. Nat Med 1:546–551CrossRefPubMed Duncan C, Dougall H, Johnston P, Green S, Brogan R, Leifert C, Smith L, Golden M, Benjamin N (1995) Chemical generation of nitric oxide in the mouth from the enterosalivary circulation of dietary nitrate. Nat Med 1:546–551CrossRefPubMed
go back to reference Ferguson SK, Holdsworth CT, Wright JL, Fees AJ, Allen JD, Jones AM, Musch TI, Poole DC (2015) Microvascular oxygen pressures in muscles comprised of different fiber types: impact of dietary nitrate supplementation. Nitric Oxide 48:38–43. doi:10.1016/j.niox.2014.09.157 CrossRefPubMed Ferguson SK, Holdsworth CT, Wright JL, Fees AJ, Allen JD, Jones AM, Musch TI, Poole DC (2015) Microvascular oxygen pressures in muscles comprised of different fiber types: impact of dietary nitrate supplementation. Nitric Oxide 48:38–43. doi:10.​1016/​j.​niox.​2014.​09.​157 CrossRefPubMed
go back to reference Govoni M, Jansson EA, Weitzberg E, Lundberg JO (2008) The increase in plasma nitrite after a dietary nitrate load is markedly attenuated by an antibacterial mouthwash. Nitric Oxide Biol Chem Off J Nitric Oxide Soc 19:333–337. doi:10.1016/j.niox.2008.08.003 CrossRef Govoni M, Jansson EA, Weitzberg E, Lundberg JO (2008) The increase in plasma nitrite after a dietary nitrate load is markedly attenuated by an antibacterial mouthwash. Nitric Oxide Biol Chem Off J Nitric Oxide Soc 19:333–337. doi:10.​1016/​j.​niox.​2008.​08.​003 CrossRef
go back to reference Hopkins WG (2007) A spreadsheet for deriving a confidence interval, mechanistic inference and clinical inference from a P value. Sportscience 11:16–20. Hopkins WG (2007) A spreadsheet for deriving a confidence interval, mechanistic inference and clinical inference from a P value. Sportscience 11:16–20.
go back to reference Issekutz B, Birkhead NC, Rodahl K (1962) Use of respiratory quotients in assessment of aerobic work capacity. J Appl Physiol 17:47–50 Issekutz B, Birkhead NC, Rodahl K (1962) Use of respiratory quotients in assessment of aerobic work capacity. J Appl Physiol 17:47–50
go back to reference Jones AM (2007) Middle- and long-distance running. In: Winter EM, Jones AM, Davison R, Bromley PD, Mercer TH (eds) Sport and exercise physiology testing guidelines: volume I—sport testing. The British Association of Sport and Exercise Sciences Guide. Routledge, London Jones AM (2007) Middle- and long-distance running. In: Winter EM, Jones AM, Davison R, Bromley PD, Mercer TH (eds) Sport and exercise physiology testing guidelines: volume I—sport testing. The British Association of Sport and Exercise Sciences Guide. Routledge, London
go back to reference Lane SC, Hawley JA, Desbrow B, Jones AM, Blackwell JR, Ross ML, Zemski AJ, Burke LM (2014) Single and combined effects of beetroot juice and caffeine supplementation on cycling time trial performance. Appl Physiol Nutr Metab 39:1050–1057. doi:10.1139/apnm-2013-0336 CrossRefPubMed Lane SC, Hawley JA, Desbrow B, Jones AM, Blackwell JR, Ross ML, Zemski AJ, Burke LM (2014) Single and combined effects of beetroot juice and caffeine supplementation on cycling time trial performance. Appl Physiol Nutr Metab 39:1050–1057. doi:10.​1139/​apnm-2013-0336 CrossRefPubMed
go back to reference Lansley KE, Winyard PG, Bailey SJ, Vanhatalo A, Wilkerson DP, Blackwell JR, Gilchrist M, Benjamin N, Jones AM (2011) Acute dietary nitrate supplementation improves cycling time trial performance. Med Sci Sports Exerc 43:1125–1131. doi:10.1249/MSS.0b013e31821597b4 CrossRefPubMed Lansley KE, Winyard PG, Bailey SJ, Vanhatalo A, Wilkerson DP, Blackwell JR, Gilchrist M, Benjamin N, Jones AM (2011) Acute dietary nitrate supplementation improves cycling time trial performance. Med Sci Sports Exerc 43:1125–1131. doi:10.​1249/​MSS.​0b013e31821597b4​ CrossRefPubMed
go back to reference McMahon NF, Leveritt MD, Pavey TG (2016) The effect of dietary nitrate supplementation on endurance exercise performance in healthy adults: A systematic review and meta-analysis. Sports Med. doi:10.1007/s40279-016-0617-7 McMahon NF, Leveritt MD, Pavey TG (2016) The effect of dietary nitrate supplementation on endurance exercise performance in healthy adults: A systematic review and meta-analysis. Sports Med. doi:10.​1007/​s40279-016-0617-7
go back to reference Midgley AW, McNaughton LR, Polman R, Marchant D (2007) Criteria for determination of maximal oxygen uptake: a brief critique and recommendations for future research. Sports Med 37:1019–1028CrossRefPubMed Midgley AW, McNaughton LR, Polman R, Marchant D (2007) Criteria for determination of maximal oxygen uptake: a brief critique and recommendations for future research. Sports Med 37:1019–1028CrossRefPubMed
go back to reference Nagababu E, Rifkind JM (2010) Measurement of plasma nitrite by chemiluminescence. In: Uppu RM, Murthy SN, Pryor WA, Parinandi NL (eds) Free radicals and antioxidant protocols. Humana, Totowa, pp 41–49CrossRef Nagababu E, Rifkind JM (2010) Measurement of plasma nitrite by chemiluminescence. In: Uppu RM, Murthy SN, Pryor WA, Parinandi NL (eds) Free radicals and antioxidant protocols. Humana, Totowa, pp 41–49CrossRef
go back to reference Pawlak-Chaouch M, Boissière J, Gamelin FX, Cuvelier G, Berthoin S, Aucouturier J (2016) Effect of dietary nitrate supplementation on metabolic rate during rest and exercise in human: a systematic review and a meta-analysis. Nitric Oxide 53:65–76. doi:10.1016/j.niox.2016.01.001 CrossRefPubMed Pawlak-Chaouch M, Boissière J, Gamelin FX, Cuvelier G, Berthoin S, Aucouturier J (2016) Effect of dietary nitrate supplementation on metabolic rate during rest and exercise in human: a systematic review and a meta-analysis. Nitric Oxide 53:65–76. doi:10.​1016/​j.​niox.​2016.​01.​001 CrossRefPubMed
go back to reference Peacock O, Tjønna AE, James P, Wisløff U, Welde B, Böhlke N, Smith A, Stokes K, Cook C, Sandbakk Ø (2012) Dietary nitrate does not enhance running performance in elite cross-country skiers. Med Sci Sports Exerc 44:2213–2219. doi:10.1249/MSS.0b013e3182640f48 CrossRefPubMed Peacock O, Tjønna AE, James P, Wisløff U, Welde B, Böhlke N, Smith A, Stokes K, Cook C, Sandbakk Ø (2012) Dietary nitrate does not enhance running performance in elite cross-country skiers. Med Sci Sports Exerc 44:2213–2219. doi:10.​1249/​MSS.​0b013e3182640f48​ CrossRefPubMed
go back to reference Porcelli S, Ramaglia M, Bellistri G, Pavei G, Pugliese L, Montorsi M, Rasica L, Marzorati M (2014) Aerobic fitness affects the exercise performance responses to nitrate supplementation. Med Sci Sports Exerc. doi:10.1249/MSS.0000000000000577 Porcelli S, Ramaglia M, Bellistri G, Pavei G, Pugliese L, Montorsi M, Rasica L, Marzorati M (2014) Aerobic fitness affects the exercise performance responses to nitrate supplementation. Med Sci Sports Exerc. doi:10.​1249/​MSS.​0000000000000577​
go back to reference Richardson RS, Noyszewski EA, Kendrick KF, Leigh JS, Wagner PD (1995) Myoglobin O2 desaturation during exercise. Evidence of limited O2 transport. J Clin Invest 96:1916–1926CrossRefPubMedPubMedCentral Richardson RS, Noyszewski EA, Kendrick KF, Leigh JS, Wagner PD (1995) Myoglobin O2 desaturation during exercise. Evidence of limited O2 transport. J Clin Invest 96:1916–1926CrossRefPubMedPubMedCentral
go back to reference Russell RD, Redmann SM, Ravussin E, Hunter GR, Larson-Meyer DE (2004) Reproducibility of endurance performance on a treadmill using a preloaded time trial. Med Sci Sports Exerc 36:717–724CrossRefPubMed Russell RD, Redmann SM, Ravussin E, Hunter GR, Larson-Meyer DE (2004) Reproducibility of endurance performance on a treadmill using a preloaded time trial. Med Sci Sports Exerc 36:717–724CrossRefPubMed
go back to reference Sale DG (1987) Influence of exercise and training on motor unit activation. Exerc Sport Sci Rev 15:95–151CrossRefPubMed Sale DG (1987) Influence of exercise and training on motor unit activation. Exerc Sport Sci Rev 15:95–151CrossRefPubMed
go back to reference Shannon O, Barlow M, Duckworth L, Woods DR, Barker T, Grindrod A, Griffiths A, O’Hara JP (2016a) The reliability of a pre-loaded treadmill time-trial in moderate normobaric hypoxia. Int J Sports Med 37:825–830. doi:10.1055/s-0042-108651 CrossRefPubMed Shannon O, Barlow M, Duckworth L, Woods DR, Barker T, Grindrod A, Griffiths A, O’Hara JP (2016a) The reliability of a pre-loaded treadmill time-trial in moderate normobaric hypoxia. Int J Sports Med 37:825–830. doi:10.​1055/​s-0042-108651 CrossRefPubMed
go back to reference Shannon OM, Duckworth L, Barlow MJ, Woods D, Lara J, Siervo M, O’Hara JP (2016b) Dietary nitrate supplementation enhances high-intensity running performance in moderate normobaric hypoxia, independent of aerobic fitness. Nitric Oxide 59:63–70. doi:10.1016/j.niox.2016.08.001 CrossRefPubMed Shannon OM, Duckworth L, Barlow MJ, Woods D, Lara J, Siervo M, O’Hara JP (2016b) Dietary nitrate supplementation enhances high-intensity running performance in moderate normobaric hypoxia, independent of aerobic fitness. Nitric Oxide 59:63–70. doi:10.​1016/​j.​niox.​2016.​08.​001 CrossRefPubMed
go back to reference Taylor HL, Buskirk E, Henschel A (1955) Maximal oxygen intake as an objective measure of cardio-respiratory performance. J Appl Physiol 8:73–80PubMed Taylor HL, Buskirk E, Henschel A (1955) Maximal oxygen intake as an objective measure of cardio-respiratory performance. J Appl Physiol 8:73–80PubMed
go back to reference Vanhatalo A, Bailey SJ, Blackwell JR, DiMenna FJ, Pavey TG, Wilkerson DP, Benjamin N, Winyard PG, Jones AM (2010) Acute and chronic effects of dietary nitrate supplementation on blood pressure and the physiological responses to moderate-intensity and incremental exercise. AJP Regul Integr Comp Physiol 299:R1121–R1131. doi:10.1152/ajpregu.00206.2010 CrossRef Vanhatalo A, Bailey SJ, Blackwell JR, DiMenna FJ, Pavey TG, Wilkerson DP, Benjamin N, Winyard PG, Jones AM (2010) Acute and chronic effects of dietary nitrate supplementation on blood pressure and the physiological responses to moderate-intensity and incremental exercise. AJP Regul Integr Comp Physiol 299:R1121–R1131. doi:10.​1152/​ajpregu.​00206.​2010 CrossRef
go back to reference Wilkerson DP, Hayward GM, Bailey SJ, Vanhatalo A, Blackwell JR, Jones AM (2012) Influence of acute dietary nitrate supplementation on 50 mile time trial performance in well-trained cyclists. Eur J Appl Physiol 112:4127–4134. doi:10.1007/s00421-012-2397-6 CrossRefPubMed Wilkerson DP, Hayward GM, Bailey SJ, Vanhatalo A, Blackwell JR, Jones AM (2012) Influence of acute dietary nitrate supplementation on 50 mile time trial performance in well-trained cyclists. Eur J Appl Physiol 112:4127–4134. doi:10.​1007/​s00421-012-2397-6 CrossRefPubMed
Metadata
Title
Dietary nitrate supplementation enhances short but not longer duration running time-trial performance
Authors
Oliver Michael Shannon
Matthew John Barlow
Lauren Duckworth
Emily Williams
Georgina Wort
David Woods
Mario Siervo
John Paul O’Hara
Publication date
01-04-2017
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Applied Physiology / Issue 4/2017
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI
https://doi.org/10.1007/s00421-017-3580-6

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