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European Journal of Applied Physiology

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01-03-2016 | Original Article

Muscle damage and repeated bout effect following blood flow restricted exercise

Authors: Peter Sieljacks, Andreas Matzon, Mathias Wernbom, Steffen Ringgaard, Kristian Vissing, Kristian Overgaard

Published in: European Journal of Applied Physiology | Issue 3/2016

Abstract

Purpose

Blood-flow restricted resistance exercise training (BFRE) is suggested to be effective in rehabilitation training, but more knowledge is required about its potential muscle damaging effects. Therefore, we investigated muscle-damaging effects of BFRE performed to failure and possible protective effects of previous bouts of BFRE or maximal eccentric exercise (ECC).

Methods

Seventeen healthy young men were allocated into two groups completing two exercise bouts separated by 14 days. One group performed BFRE in both exercise bouts (BB). The other group performed ECC in the first and BFRE in the second bout. BFRE was performed to failure. Indicators of muscle damage were evaluated before and after exercise.

Results

The first bout in the BB group led to decrements in maximum isometric torque, and increases in muscle soreness, muscle water retention, and serum muscle protein concentrations after exercise. These changes were comparable in magnitude and time course to what was observed after first bout ECC. An attenuated response was observed in the repeated exercise bout in both groups.

Conclusion

We conclude that unaccustomed single-bout BFRE performed to failure induces significant muscle damage. Additionally, both ECC and BFRE can precondition against muscle damage induced by a subsequent bout of BFRE.

Armstrong RB, Warren GL, Warren JA (1991) Mechanisms of exercise-induced muscle fibre injury. Sports Med 3:184–207CrossRef

Belcastro AN, Shewchuk LD, Raj DA (1998) Exercise-induced muscle injury: a calpain hypothesis. Mol Cell Biochem 1–2:135–145CrossRef

Chen HL, Nosaka K, Chen TC (2012) Muscle damage protection by low-intensity eccentric contractions remains for 2 weeks but not 3 weeks. Eur J Appl Physiol 2:555–565. doi:10.1007/s00421-011-1999-8 CrossRef

Cumming KT, Paulsen G, Wernbom M, Ugelstad I, Raastad T (2014) Acute response and subcellular movement of HSP27, alphaB-crystallin and HSP70 in human skeletal muscle after blood-flow-restricted low-load resistance exercise. Acta Physiol (Oxf) 4:634–646. doi:10.1111/apha.12305 CrossRef

Eston RG, Finney S, Baker S, Baltzopoulos V (1996) Muscle tenderness and peak torque changes after downhill running following a prior bout of isokinetic eccentric exercise. J Sports Sci 4:291–299. doi:10.1080/02640419608727714 CrossRef

Farup J, de Paoli F, Bjerg K, Riis S, Ringgard S, Vissing K (2015) Blood flow restricted and traditional resistance training performed to fatigue produce equal muscle hypertrophy. Scand J Med Sci Sports. doi:10.1111/sms.12396

Foley JM, Jayaraman RC, Prior BM, Pivarnik JM, Meyer RA (1999) MR measurements of muscle damage and adaptation after eccentric exercise. J Appl Physiol (1985) 6:2311–2318

Fredsted A, Mikkelsen UR, Gissel H, Clausen T (2005) Anoxia induces Ca2+ influx and loss of cell membrane integrity in rat extensor digitorum longus muscle. Exp Physiol 5:703–714. doi:10.1113/expphysiol.2005.030247 CrossRef

Fredsted A, Gissel H, Madsen K, Clausen T (2007) Causes of excitation-induced muscle cell damage in isometric contractions: mechanical stress or calcium overload? Am J Physiol Regul Integr Comp Physiol 6:R2249–R2258. doi:10.1152/ajpregu.00415.2006 CrossRef

Friden J, Sjostrom M, Ekblom B (1983) Myofibrillar damage following intense eccentric exercise in man. Int J Sports Med 3:170–176CrossRef

Ingalls CP, Warren GL, Williams JH, Ward CW, Armstrong RB (1998) E–C coupling failure in mouse EDL muscle after in vivo eccentric contractions. J Appl Physiol (1985) 1:58–67

Komi PV, Kaneko M, Aura O (1987) EMG activity of the leg extensor muscles with special reference to mechanical efficiency in concentric and eccentric exercise. Int J Sports Med 8:22–29 CrossRefPubMed

Larsen RG, Ringgaard S, Overgaard K (2007) Localization and quantification of muscle damage by magnetic resonance imaging following step exercise in young women. Scand J Med Sci Sports 17(1):76–83 PubMed

Lieber RL, Friden J (1993) Muscle damage is not a function of muscle force but active muscle strain. J Appl Physiol (1985) 2:520–526

Loenneke JP, Thiebaud RS, Fahs CA, Rossow LM, Abe T, Bemben MG (2013) Blood flow restriction does not result in prolonged decrements in torque. Eur J Appl Physiol 4:923–931. doi:10.1007/s00421-012-2502-x CrossRef

Madarame H, Neya M, Ochi E, Nakazato K, Sato Y, Ishii N (2008) Cross-transfer effects of resistance training with blood flow restriction. Med Sci Sports Exerc 2:258–263. doi:10.1249/mss.0b013e31815c6d7e CrossRef

Madarame H, Kurano M, Fukumura K, Fukuda T, Nakajima T (2013) Haemostatic and inflammatory responses to blood flow-restricted exercise in patients with ischaemic heart disease: a pilot study. Clin Physiol Funct Imaging 1:11–17. doi:10.1111/j.1475-097X.2012.01158.x CrossRef

McHugh MP (2003) Recent advances in the understanding of the repeated bout effect: the protective effect against muscle damage from a single bout of eccentric exercise. Scand J Med Sci Sports 2:88–97CrossRef

Moritani T, Muramatsu S, Muro M (1987) Activity of motor units during concentric and eccentric contractions. Am J Phys Med 6:338–350

Nardone A, Romano C, Schieppati M (1989) Selective recruitment of high-threshold human motor units during voluntary isotonic lengthening of active muscles. J Physiol 409:451–471PubMedCentralCrossRefPubMed

Newham DJ, McPhail G, Mills KR, Edwards RH (1983) Ultrastructural changes after concentric and eccentric contractions of human muscle. J Neurol Sci 1:109–122CrossRef

Newham DJ, Jones DA, Clarkson PM (1987) Repeated high-force eccentric exercise: effects on muscle pain and damage. J Appl Physiol 4:1381–1386

Nielsen JL, Aagaard P, Bech RD, Nygaard T, Hvid LG, Wernbom M, Suetta C, Frandsen U (2012) Proliferation of myogenic stem cells in human skeletal muscle in response to low-load resistance training with blood flow restriction. J Physiol Pt 17:4351–4361. doi:10.1113/jphysiol.2012.237008 CrossRef

Nosaka K, Clarkson PM (1995) Muscle damage following repeated bouts of high force eccentric exercise. Med Sci Sports Exerc 9:1263–1269

Nosaka K, Clarkson PM (1996) Changes in indicators of inflammation after eccentric exercise of the elbow flexors. Med Sci Sports Exerc 8:953–961CrossRef

Nosaka K, Sakamoto K, Newton M, Sacco P (2001) How long does the protective effect on eccentric exercise-induced muscle damage last? Med Sci Sports Exerc 9:1490–1495CrossRef

Ohta H, Kurosawa H, Ikeda H, Iwase Y, Satou N, Nakamura S (2003) Low-load resistance muscular training with moderate restriction of blood flow after anterior cruciate ligament reconstruction. Acta Orthop Scand 1:62–68. doi:10.1080/00016470310013680 CrossRef

Pasiakos SM, Carbone JW (2014) Assessment of skeletal muscle proteolysis and the regulatory response to nutrition and exercise. IUBMB Life 7:478–484. doi:10.1002/iub.1291 CrossRef

Raastad T, Owe SG, Paulsen G, Enns D, Overgaard K, Crameri R, Kiil S, Belcastro A, Bergersen L, Hallen J (2010) Changes in calpain activity, muscle structure, and function after eccentric exercise. Med Sci Sports Exerc 1:86–95. doi:10.1249/MSS.0b013e3181ac7afa CrossRef

Stupka N, Tarnopolsky MA, Yardley NJ, Phillips SM (2001) Cellular adaptation to repeated eccentric exercise-induced muscle damage. J Appl Physiol (1985) 4:1669–1678

Suga T, Okita K, Morita N, Yokota T, Hirabayashi K, Horiuchi M, Takada S, Omokawa M, Kinugawa S, Tsutsui H (2010) Dose effect on intramuscular metabolic stress during low-intensity resistance exercise with blood flow restriction. J Appl Physiol (1985) 6:1563–1567. doi:10.1152/japplphysiol.00504.2009 CrossRef

Takahashi H, Kuno S, Miyamoto T, Yoshioka H, Inaki M, Akima H, Katsuta S, Anno I, Itai Y (1994) Changes in magnetic resonance images in human skeletal muscle after eccentric exercise. Eur J Appl Physiol Occup Physiol 5:408–413CrossRef

Takarada Y, Nakamura Y, Aruga S, Onda T, Miyazaki S, Ishii N (2000) Rapid increase in plasma growth hormone after low-intensity resistance exercise with vascular occlusion. J Appl Physiol (1985) 1:61–65

Thiebaud RS, Yasuda T, Loenneke JP, Abe T (2013) Effects of low-intensity concentric and eccentric exercise combined with blood flow restriction on indices of exercise-induced muscle damage. Interv Med Appl Sci 2:53–59. doi:10.1556/IMAS.5.2013.2.1 CrossRef

Thiebaud RS, Loenneke JP, Fahs CA, Kim D, Ye X, Abe T, Nosaka K, Bemben MG (2014) Muscle damage after low-intensity eccentric contractions with blood flow restriction. Acta Physiol Hung 2:150–157. doi:10.1556/APhysiol.101.2014.2.3 CrossRef

Umbel JD, Hoffman RL, Dearth DJ, Chleboun GS, Manini TM, Clark BC (2009) Delayed-onset muscle soreness induced by low-load blood flow-restricted exercise. Eur J Appl Physiol 6:687–695. doi:10.1007/s00421-009-1175-6 CrossRef

Vissing K, Overgaard K, Nedergaard A, Fredsted A, Schjerling P (2008) Effects of concentric and repeated eccentric exercise on muscle damage and calpain–calpastatin gene expression in human skeletal muscle. Eur J Appl Physiol 3:323–332. doi:10.1007/s00421-008-0709-7 CrossRef

Warren GL, Lowe DA, Armstrong RB (1999) Measurement tools used in the study of eccentric contraction-induced injury. Sports Med 1:43–59CrossRef

Wernbom M, Jarrebring R, Andreasson MA, Augustsson J (2009) Acute effects of blood flow restriction on muscle activity and endurance during fatiguing dynamic knee extensions at low load. J Strength Cond Res 8:2389–2395. doi:10.1519/JSC.0b013e3181bc1c2a CrossRef

Wernbom M, Paulsen G, Nilsen TS, Hisdal J, Raastad T (2012) Contractile function and sarcolemmal permeability after acute low-load resistance exercise with blood flow restriction. Eur J Appl Physiol 6:2051–2063. doi:10.1007/s00421-011-2172-0 CrossRef

Wilson JM, Lowery RP, Joy JM, Loenneke JP, Naimo MA (2013) Practical blood flow restriction training increases acute determinants of hypertrophy without increasing indices of muscle damage. J Strength Cond Res 11:3068–3075. doi:10.1519/JSC.0b013e31828a1ffa CrossRef

Zhang BT, Yeung SS, Allen DG, Qin L, Yeung EW (2008) Role of the calcium–calpain pathway in cytoskeletal damage after eccentric contractions. J Appl Physiol (1985) 1:352–357. doi:10.1152/japplphysiol.90320.2008 CrossRef

Title: Muscle damage and repeated bout effect following blood flow restricted exercise
Authors: Peter Sieljacks
Andreas Matzon
Mathias Wernbom
Steffen Ringgaard
Kristian Vissing
Kristian Overgaard
Publication date: 01-03-2016
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Applied Physiology / Issue 3/2016
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-015-3304-8

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Springer Medicine

Muscle damage and repeated bout effect following blood flow restricted exercise

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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