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01-10-2013 | Original Article

Acute effects of sex-specific sex hormones on heat shock proteins in fast muscle of male and female rats

Authors: William A. Romani, David W. Russ

Published in: European Journal of Applied Physiology | Issue 10/2013

Abstract

Heat shock protein (HSP) expression and sex hormone levels have been shown to influence several aspects of skeletal muscle physiology (e.g., hypertrophy, resistance to oxidative stress), suggesting that sex hormone levels can effect HSP expression. This study evaluated the effects of differing levels of sex-specific sex hormones (i.e., testosterone in males and estrogen in females) on the expression of 4: HSP70, HSC70, HSP25, and αB-crystallin in the quadriceps muscles of male and female rats. Animals were assigned to 1 of 3 groups (n = 5 M and F/group). The first group (Ctl) consisted of typically cage-housed animals that served as controls. The second group (H) was gonadectomized and received either testosterone (males) or estradiol (females) via injection for 12 consecutive days. The third group (Gx) was gonadectomized and injected as above, but with vehicle only, rather than hormones. Significant sex by condition interactions (P < 0.05 by two-way MANOVA) were found for all 4 proteins studied, except for HSP70, which exhibited a significant effect of condition only. The expression of all HSPs was greater (1.9–2.5-fold) in males vs. females in the Ctl group, except for HSP70, which was no different. Generally, gonadectomy appeared to have greater effects in males than females, but administration of the exogenous sex hormones tended to produce more robust relative changes in females than males. There were no differences in myosin composition in any of the groups, suggesting that changes in fiber type were not a factor in the differential protein expression. These data may have implications for sex-related differences in muscular responses to exercise, disuse, and injury.

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Title: Acute effects of sex-specific sex hormones on heat shock proteins in fast muscle of male and female rats
Authors: William A. Romani
David W. Russ
Publication date: 01-10-2013
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Applied Physiology / Issue 10/2013
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-013-2686-8

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Acute effects of sex-specific sex hormones on heat shock proteins in fast muscle of male and female rats

Abstract

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