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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-07-2010 | Basic Science

IL-2 and IFN-gamma in the retina of diabetic rats

Authors: Siv Johnsen-Soriano, María Sancho-Tello, Emma Arnal, Amparo Navea, Enrique Cervera, Francisco Bosch-Morell, Maria Miranda, Francisco Javier Romero

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 7/2010

Abstract

Background

The pathophysiology of the early events leading to diabetic retinopathy is not fully understood. It has been suggested that Inflammatory processes are involved in the development of the disease; however, the concentrations of tissue retinal inflammatory mediators and their possible alteration in diabetic retinopathy have not been described. The aim of this work was to study T-helper cell cytokine and chemokine profiles, and tyrosine nitration in retinal tissue of diabetic rats.

Methods

Cytokines (interleukin IL-1a, IL-1b, IL-2, IL-4, IL-6, IL-10, TNFa, GM-CSF, IFN-g), chemokines (MIP-1a, MIP-2, MIP-3a, MCP-1, GRO/KC, RANTES, Fractalkine), and tyrosine nitration were measured in retinal homogenate obtained from Long–Evans rats after 5 months of experimental diabetes.

Results

The T-helper type 1 cytokines IL-2 and INF-gamma, in addition to NO production (measured as nitrotyrosine), were found to be significantly elevated in diabetic rat retina homogenates. None of the other cytokines and chemokines studied were affected by the diabetic condition.

Conclusions

Immunoregulatory cytokines belonging to the Th-1 group (IL-2 and IFN-gamma) were increased in the retina of experimental diabetic rats. Moreover, the nitrotyrosine formation (as an expression of increased NO production) was significantly elevated in the diabetic retina, supporting the concept of an inflammatory element in the development of diabetic retinopathy.

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Title: IL-2 and IFN-gamma in the retina of diabetic rats
Authors: Siv Johnsen-Soriano
María Sancho-Tello
Emma Arnal
Amparo Navea
Enrique Cervera
Francisco Bosch-Morell
Maria Miranda
Francisco Javier Romero
Publication date: 01-07-2010
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 7/2010
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-009-1289-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

IL-2 and IFN-gamma in the retina of diabetic rats

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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