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01-05-2007 | Original Article

Development of species identification tests targeting the 16S ribosomal RNA coding region in mitochondrial DNA

Authors: Kazuhiko Imaizumi, Tomoko Akutsu, Sachio Miyasaka, Mineo Yoshino

Published in: International Journal of Legal Medicine | Issue 3/2007

Abstract

This report describes the development of a species testing system based on the diversity of nucleotide sequences in mitochondrial DNA (mtDNA) among species. Five species, human, cow, pig, dog, and cat, were considered. The partial nucleotide sequences in 16S ribosomal RNA coding region were chosen as the target for discriminating the species. The sequence diversities of this approximately 400 bp long region ranged from 15.7 to 24.1% among the five species. Sequencing analysis of this target on 50 individuals of each species (53 for dogs) revealed that the nucleotide sequences were well preserved within species. Species-specific PCR for each species was also designed, and satisfactory results with regard to both sensitivity and specificity were obtained. A validation study with DNA extracted from bovine bone exposed to the environment revealed that the PCRs designed in this study worked correctly. From the results obtained, it was shown that this testing system could be a good tool for species identification. One successful case report is also demonstrated.

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Title: Development of species identification tests targeting the 16S ribosomal RNA coding region in mitochondrial DNA
Authors: Kazuhiko Imaizumi
Tomoko Akutsu
Sachio Miyasaka
Mineo Yoshino
Publication date: 01-05-2007
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Legal Medicine / Issue 3/2007
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-006-0127-5

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Development of species identification tests targeting the 16S ribosomal RNA coding region in mitochondrial DNA

Abstract

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