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01-08-2003 | Technical Note

Age estimation in dental pulp DNA based on human telomere shortening

Authors: Tomoya Takasaki, Akiko Tsuji, Noriaki Ikeda, Masamichi Ohishi

Published in: International Journal of Legal Medicine | Issue 4/2003

Abstract

Age estimation based on evidence found in teeth has received considerable attention within the field of forensic science. We determined the terminal restriction fragment (TRF) length, as telomere length, to estimate age. Using dental pulp DNA we found the average TRF length showed a tendency to shortening with aging. Our findings show that telomere shortening, based on dental pulp DNA is a new and useful approach to estimate age of the subject at the time of death.

Acil Y, Springer ING, Prasse JG, Hedderich J, Jepsen S (2002) Concentration of collagen cross-links in human dentin bears no relation to the individual age. Int J Legal Med 116:340–343PubMed

Baccino E, Ubelaker DH, Hayek LC, Zerilli A (1999) Evaluation of seven methods of estimating age at death from mature human skeletal remains. J Forensic Sci 44:931–936PubMed

Foti B, Adalian P, Signoli M, Ardagna Y, Dutour O, Leonetti G (2001) Limits of the Lamendin method in age determination. Forensic Sci Int 122:101–106CrossRefPubMed

Gustafson G (1950) Age determinations on teeth. J Am Dent Assoc 41:45–54

Harley CB, Futcher AB, Greider CW (1990) Telomeres shorten during ageing of human fibroblasts. Nature 345:458–460PubMed

Hastie ND, Dempster M, Dunlop MG, Thompson AM, Green DK, Allshire RC (1990) Telomere reduction in human colorectal carcinoma and with ageing. Nature 346:866–86

Kvaal SI, Kolltveit KM, Thomsen IO, Solheim T (1995) Age estimation of adults from dental radiographs. Forensic Sci Int 74:175–185CrossRefPubMed

Lamendin H, Baccino E, Humbert JF, Tavernier JC, Nossintchouk RM, Zerilli A (1992) A simple technique for age estimation in adult corpses: the two criteria dental method. J Forensic Sci 37:1373–1379PubMed

Martin-de las Heras S, Valenzuela A, Villanueva E (1999) Deoxypridinoline crosslinks in human dentin and estimation of age. Int J Legal Med 112:222–226PubMed

10.

Moyzis RK, Buckingham JM, Cram LS et al. (1988) A highly conserved repetitive DNA sequence, (TTAGGG)_n, present at the telomeres of human chromosomes. Proc Natl Acad Sci USA 85:6622–6626PubMed

11.

Mönstad H, Pfeifffer H, Teivens A (1994) Estimation of dental age using HPLC-technique to determine the degree of aspartic acid racemization. J Forensic Sci 39:1425–1431PubMed

12.

Ohtani S (2002) Technical notes for age estimation using the femur: influence of various analytical conditions on D-aspartic acid contents. Int J Legal Med 116:361–364PubMed

13.

Pfeiffer H, Hühne J, Seitz B, Brinkmann B (1999) Influence of soil storage and exposure period on DNA recovery from teeth. Int J Legal Med 112:142–144PubMed

14.

Ritz-Timme S, Cattaneo C, Collins MJ, Waite ER, Schütz HW, Kaatsch H-J (2000) Age estimation: the state of the art in relation to the specific demands of forensic practise. Int J Legal Med 113:129–136PubMed

15.

Smith BC, Fisher DL, Weedn VW, Warnock GR, Holland MM (1993) A systematic approach to the sampling of dental DNA. J Forensic Sci 38:1194–1209PubMed

16.

Solheim T, Sundnes PK (1980) Dental age estimation of Norwegian adults. A comparison of different methods. Forensic Sci Int 16:7–17PubMed

17.

Sweet DJ, Sweet CHW (1995) DNA analysis of dental pulp to link incinerated remains of homicide victim to crime scene. J Forensic Sci 40:310–314PubMed

18.

Tsuji A, Ishiko A, Takasaki T, Ikeda N (2002) Estimating age of humans based on telomere shortening. Forensic Sci Int 126:197–199CrossRefPubMed

19.

Zakian VA (1995) Telomeres: beginning to understand the end. Science 270:1601–1607PubMed

Title: Age estimation in dental pulp DNA based on human telomere shortening
Authors: Tomoya Takasaki
Akiko Tsuji
Noriaki Ikeda
Masamichi Ohishi
Publication date: 01-08-2003
Publisher: Springer-Verlag
Published in: International Journal of Legal Medicine / Issue 4/2003
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-003-0376-5

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Age estimation in dental pulp DNA based on human telomere shortening

Abstract

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Abstract

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