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Archives of Gynecology and Obstetrics

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01-01-2014 | Gynecologic Oncology

Establishment of Fischer 344 rat model of ovarian cancer with lymphatic metastasis

Authors: Lingling Fan, Yingtao Liu, Xiaoyan Zhang, Yu Kang, Congjian Xu

Published in: Archives of Gynecology and Obstetrics | Issue 1/2014

Abstract

Purpose

To establish a model of ovarian cancer with highly lymphatic metastasis in immunocompetent rats.

Methods

Thirty-two female Fischer 344 rats were divided randomly and equally into two groups: footpad group and intraperitoneal (i.p.) group. At 8 weeks after injection with NuTu-19 ovarian cancer cells, lymphatic metastasis were analyzed by pathohistology; body weight was monitored per week, Survival curves were determined by Kaplan–Meier analysis.

Results

Footpad injection could efficiently generate the lymphatic metastasis; specifically, the incidences of metastasis in the ipsilateral popliteal, inguinal and para-iliac lymph nodes were 100 % (8/8), 75 % (6/8), and 37.5 % (3/8), respectively. The mean volume and weight of the ipsilateral popliteal lymph nodes were 0.405 ± 0.096 cm³ and 0.418 ± 0.118 g in footpad group. However, no lymphatic metastasis lesions were found in i.p. group. Moreover, Kaplan–Meier analysis showed that the average survival time of the footpad group was significantly longer than that of the i.p. group (18.429 ± 1.112 vs. 10.286 ± 0.505 weeks).

Conclusions

Our experiments suggest that footpad injection is a very efficient method to generate ovarian cancer with lymphatic metastasis in an immune-competent animal, and we believe that this model will be very helpful for shedding light on the mechanism of lymphogenous metastasis and developing novel therapeutic targets for ovarian cancer patients.

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Title: Establishment of Fischer 344 rat model of ovarian cancer with lymphatic metastasis
Authors: Lingling Fan
Yingtao Liu
Xiaoyan Zhang
Yu Kang
Congjian Xu
Publication date: 01-01-2014
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 1/2014
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-013-2937-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Establishment of Fischer 344 rat model of ovarian cancer with lymphatic metastasis

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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