Top

Archives of Gynecology and Obstetrics

Published in:

01-10-2013 | Maternal-Fetal Medicine

Proteomic analysis of preterm premature rupture of membranes in placental tissue

Authors: Aimin Chang, Zhan Zhang, Linlin Zhang, Yan Gao, Ling Zhang, Liting Jia, Shihong Cui, Peng Wang

Published in: Archives of Gynecology and Obstetrics | Issue 4/2013

Abstract

Objection

Preterm premature rupture of membranes (PPROM) is an obstetrics complication and is the leading cause of perinatal mortality and morbidity. PPROM results in critical care emergencies, and nearly all PPROM events are spontaneous and unpredictable. In addition, changes in the proteome in placental tissue during pregnancy that lead to PPROM are not clear.

Methods

We utilize a proteomics approach to study the molecular mechanisms behind human PPROM. A better understanding of proteome alteration could lead to the identification of better diagnostic/prognostic markers. Human placental tissue was collected in clearly differentiated cases of PPROM and in a healthy term control. Two-dimensional gel polyacrylamide electrophoresis coupled with mass spectrometry and bioinformatics analysis was utilized to identify proteins with altered expression.

Results

In this study, only the most important protein differences were selected for further analysis. Most of the identified proteins were structural/cytoskeletal components of the cell or involved in the regulation of energy metabolism and oxidative stress.

Conclusions

As a result, this approach has led to the identification of several proteins involved in the underlying pathophysiological mechanisms that can further serve as novel diagnostic tools and targets for rational drug intervention.

Parry S, Fau-Strauss JF, 3rd, Strauss JF, 3rd (1998) Premature rupture of the fetal membranes (0028-4793, Print)

Locatelli A, Ghidini A, Paterlini G, Patane L, Doria V, Zorloni C, Pezzullo JC (2005) Gestational age at preterm premature rupture of membranes: a risk factor for neonatal white matter damage. Am J Obstet Gynecol 193(3 Pt 2):947–951. doi:10.1016/j.ajog.2005.06.039 PubMedCrossRef

Rosen T, Kuczynski E, O’Neill LM, Funai EF, Lockwood CJ (2001) Plasma levels of thrombin-antithrombin complexes predict preterm premature rupture of the fetal membranes. J Matern Fetal Med 10(5):297–300PubMedCrossRef

Caughey AB, Robinson JN, Norwitz ER (2008) Contemporary diagnosis and management of preterm premature rupture of membranes. Rev Obstet Gynecol 1(1):11–22PubMed

Gomez-Lopez N, Laresgoiti-Servitje E, Olson DM, Estrada-Gutierrez G, Vadillo-Ortega F (2010) The role of chemokines in term and premature rupture of the fetal membranes: a review. Biol Reprod 82(5):809–814. doi:10.1095/biolreprod.109.080432 PubMedCrossRef

Williams MA, Mittendorf R, Stubblefield PG, Lieberman E, Schoenbaum SC, Monson RR (1992) Cigarettes, coffee, and preterm premature rupture of the membranes. Am J Epidemiol 135(8):895–903PubMed

Ekwo EE, Gosselink CA, Woolson R, Moawad A (1993) Risks for premature rupture of amniotic membranes. Int J Epidemiol 22(3):495–503PubMedCrossRef

Guinn DA, Goldenberg RL, Hauth JC, Andrews WW, Thom E, Romero R (1995) Risk factors for the development of preterm premature rupture of the membranes after arrest of preterm labor. Am J Obstet Gynecol 173(4):1310–1315. doi:0002-9378(95)91377-7 PubMedCrossRef

Wang H, Parry S, Macones G, Sammel MD, Kuivaniemi H, Tromp G, Argyropoulos G, Halder I, Shriver MD, Romero R, Strauss JF 3rd (2006) A functional SNP in the promoter of the SERPINH1 gene increases risk of preterm premature rupture of membranes in African Americans. Proc Natl Acad Sci USA 103(36):13463–13467. doi:10.1073/pnas.0603676103 PubMedCrossRef

10.

Lockwood CJ, Kuczynski E (2001) Risk stratification and pathological mechanisms in preterm delivery. Paediatr Perinat Epidemiol 15(Suppl 2):78–89. doi:ppe010 PubMedCrossRef

11.

Butt RH, Lee MW, Pirshahid SA, Backlund PS, Wood S, Coorssen JR (2006) An initial proteomic analysis of human preterm labor: placental membranes. J Proteome Res 5(11):3161–3172. doi:10.1021/pr060282n PubMedCrossRef

12.

Vuadens F, Benay C, Crettaz D, Gallot D, Sapin V, Schneider P, Bienvenut WV, Lemery D, Quadroni M, Dastugue B, Tissot JD (2003) Identification of biologic markers of the premature rupture of fetal membranes: proteomic approach. Proteomics 3(8):1521–1525. doi:10.1002/pmic.200300455 PubMedCrossRef

13.

Thadikkaran L, Crettaz D, Siegenthaler MA, Gallot D, Sapin V, Iozzo RV, Queloz PA, Schneider P, Tissot JD (2005) The role of proteomics in the assessment of premature rupture of fetal membranes. Clin Chim Acta 360(1–2):27–36. doi:10.1016/j.cccn.2005.04.018 PubMedCrossRef

14.

Robinson JM, Ackerman WEt, Kniss DA, Takizawa T, Vandre DD (2008) Proteomics of the human placenta: promises and realities. Placenta 29(2):135–143. doi:10.1016/j.placenta.2007.12.005 PubMedCrossRef

15.

Robinson JM, Vandre DD, Ackerman WEt (2009) Placental proteomics: a shortcut to biological insight. Placenta 30(Suppl A):S83–S89. doi:10.1016/j.placenta.2008.10.017

16.

Churchward MA, Butt RH, Lang JC, Hsu KK, Coorssen JR (2005) Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis. Proteome Sci 3(1):5. doi:10.1186/1477-5956-3-5 PubMedCrossRef

17.

Butt RH, Coorssen JR (2005) Postfractionation for enhanced proteomic analyses: routine electrophoretic methods increase the resolution of standard 2D-PAGE. J Proteome Res 4(3):982–991. doi:10.1021/pr050054d PubMedCrossRef

18.

Davis MT, Stahl DC, Hefta SA, Lee TD (1995) A microscale electrospray interface for on-line, capillary liquid chromatography/tandem mass spectrometry of complex peptide mixtures. Anal Chem 67(24):4549–4556PubMedCrossRef

19.

Cui S, Hu J, Yang B, Shi L, Huang F, Tsai SN, Ngai SM, He Y, Zhang J (2009) Proteomic characterization of Phragmites communis in ecotypes of swamp and desert dune. Proteomics 9(16):3950–3967. doi:10.1002/pmic.200800654 PubMedCrossRef

20.

Perkins DN, Pappin DJ, Creasy DM, Cottrell JS (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20(18):3551–3567. doi:10.1002/(SICI)1522-2683(19991201)20:18<3551:AID-ELPS3551>3.0.CO;2-2 PubMedCrossRef

21.

Carroll SG, Blott M, Nicolaides KH (1995) Preterm prelabor amniorrhexis: outcome of live births. Obstet Gynecol 86(1):18–25. doi:10.1016/0029-7844(95)00085-6 PubMedCrossRef

22.

Pasquier JC, Picaud JC, Rabilloud M, Claris O, Ecochard R, Moret S, Mellier G (2009) Neonatal outcomes after elective delivery management of preterm premature rupture of the membranes before 34 weeks’ gestation (DOMINOS study). Eur J Obstet Gynecol Reprod Biol 143(1):18–23. doi:10.1016/j.ejogrb.2008.10.017 PubMedCrossRef

23.

Gygi SP, Corthals GL, Zhang Y, Rochon Y, Aebersold R (2000) Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology. Proc Natl Acad Sci USA 97(17):9390–9395. doi:10.1073/pnas.160270797 PubMedCrossRef

24.

Garbis S, Lubec G, Fountoulakis M (2005) Limitations of current proteomics technologies. J Chromatogr A 1077(1):1–18PubMedCrossRef

25.

Righetti PG, Castagna A, Herbert B, Candiano G (2005) How to bring the “unseen” proteome to the limelight via electrophoretic pre-fractionation techniques. Biosci Rep 25(1–2):3–17. doi:10.1007/s10540-005-2844-2 PubMedCrossRef

26.

Schober EA, Kusy RP, Savitz DA (1994) Resistance of fetal membranes to concentrated force applications and reconciliation of puncture and burst testing. Ann Biomed Eng 22(5):540–548PubMedCrossRef

27.

Buhimschi IA, Kramer WB, Buhimschi CS, Thompson LP, Weiner CP (2000) Reduction-oxidation (redox) state regulation of matrix metalloproteinase activity in human fetal membranes. Am J Obstet Gynecol 182(2):458–464. doi:S0002937800504359 PubMedCrossRef

28.

Menon R, Fortunato SJ (2004) The role of matrix degrading enzymes and apoptosis in rupture of membranes. J Soc Gynecol Investig 11(7):427–437. doi:10.1016/j.jsgi.2004.04.001 PubMedCrossRef

29.

Draper D, McGregor J, Hall J, Jones W, Beutz M, Heine RP, Porreco R (1995) Elevated protease activities in human amnion and chorion correlate with preterm premature rupture of membranes. Am J Obstet Gynecol 173(5):1506–1512. doi:0002-9378(95)90640-1 PubMedCrossRef

30.

Michel PE, Crettaz D, Morier P, Heller M, Gallot D, Tissot JD, Reymond F, Rossier JS (2006) Proteome analysis of human plasma and amniotic fluid by Off-Gel isoelectric focusing followed by nano-LC–MS/MS. Electrophoresis 27(5–6):1169–1181. doi:10.1002/elps.200500680 PubMedCrossRef

31.

Neuer A, Spandorfer SD, Giraldo P, Dieterle S, Rosenwaks Z, Witkin SS (2000) The role of heat shock proteins in reproduction. Hum Reprod Update 6(2):149–159PubMedCrossRef

32.

Jaattela M, Wissing D, Kokholm K, Kallunki T, Egeblad M (1998) Hsp70 exerts its anti-apoptotic function downstream of caspase-3-like proteases. EMBO J 17(21):6124–6134. doi:10.1093/emboj/17.21.6124 PubMedCrossRef

33.

Thornberry NA (1998) Caspases: key mediators of apoptosis. Chem Biol 5(5):R97–R103. doi:S1074-5521(98)90615-9 PubMedCrossRef

34.

Georgopoulos C, Welch WJ (1993) Role of the major heat shock proteins as molecular chaperones. Annu Rev Cell Biol 9:601–634. doi:10.1146/annurev.cb.09.110193.003125 PubMedCrossRef

35.

Dix DJ, Allen JW, Collins BW, Mori C, Nakamura N, Poorman-Allen P, Goulding EH, Eddy EM (1996) Targeted gene disruption of Hsp70-2 results in failed meiosis, germ cell apoptosis, and male infertility. Proc Natl Acad Sci USA 93(8):3264–3268PubMedCrossRef

36.

Gelber SE, Bongiovanni AM, Jean-Pierre C, Linhares IM, Skupski DW, Witkin SS (2007) Antibodies to the 70 kDa heat shock protein in midtrimester amniotic fluid and intraamniotic immunity. Am J Obstet Gynecol 197(3):278 e271–278 e274. doi:10.1016/j.ajog.2007.06.019 CrossRef

37.

Jean-Pierre C, Perni SC, Bongiovanni AM, Kalish RB, Karasahan E, Ravich M, Ratushny V, Skupski DW, Witkin SS (2006) Extracellular 70-kD heat shock protein in mid-trimester amniotic fluid and its effect on cytokine production by ex vivo-cultured amniotic fluid cells. Am J Obstet Gynecol 194(3):694–698. doi:10.1016/j.ajog.2006.01.066 PubMedCrossRef

38.

Scannell M, Flanagan MB, deStefani A, Wynne KJ, Cagney G, Godson C, Maderna P (2007) Annexin-1 and peptide derivatives are released by apoptotic cells and stimulate phagocytosis of apoptotic neutrophils by macrophages. J Immunol 178(7):4595–4605. doi:178/7/4595 PubMed

39.

Maderna P, Yona S, Perretti M, Godson C (2005) Modulation of phagocytosis of apoptotic neutrophils by supernatant from dexamethasone-treated macrophages and annexin-derived peptide Ac(2-26). J Immunol 174(6):3727–3733. doi:174/6/3727 PubMed

40.

Hu N, Flaig MJ, Su H, Shou JZ, Roth MJ, Li WJ, Wang C, Goldstein AM, Li G, Emmert-Buck MR, Taylor PR (2004) Comprehensive characterization of annexin I alterations in esophageal squamous cell carcinoma. Clin Cancer Res 10(18 Pt 1):6013–6022. doi:10.1158/1078-0432.CCR-04-0317 PubMedCrossRef

41.

Xin W, Rhodes DR, Ingold C, Chinnaiyan AM, Rubin MA (2003) Dysregulation of the annexin family protein family is associated with prostate cancer progression. Am J Pathol 162(1):255–261. doi:10.1016/S0002-9440(10)63816-3 PubMedCrossRef

42.

de Coupade C, Gillet R, Bennoun M, Briand P, Russo-Marie F, Solito E (2000) Annexin 1 expression and phosphorylation are upregulated during liver regeneration and transformation in antithrombin III SV40 T large antigen transgenic mice. Hepatology 31(2):371–380. doi:10.1002/hep.510310217 PubMedCrossRef

43.

Bai XF, Ni XG, Zhao P, Liu SM, Wang HX, Guo B, Zhou LP, Liu F, Zhang JS, Wang K, Xie YQ, Shao YF, Zhao XH (2004) Overexpression of annexin 1 in pancreatic cancer and its clinical significance. World J Gastroenterol 10(10):1466–1470PubMed

44.

Masuda J, Takayama E, Satoh A, Ida M, Shinohara T, Kojima-Aikawa K, Ohsuzu F, Nakanishi K, Kuroda K, Murakami M, Suzuki K, Matsumoto I (2004) Levels of annexin IV and V in the plasma of pregnant and postpartum women. Thromb Haemost 91(6):1129–1136. doi:10.1267/THRO04061129 PubMed

45.

Prosperi MT, Ferbus D, Karczinski I, Goubin G (1993) A human cDNA corresponding to a gene overexpressed during cell proliferation encodes a product sharing homology with amoebic and bacterial proteins. J Biol Chem 268(15):11050–11056PubMed

46.

Zhang P, Liu B, Kang SW, Seo MS, Rhee SG, Obeid LM (1997) Thioredoxin peroxidase is a novel inhibitor of apoptosis with a mechanism distinct from that of Bcl-2. J Biol Chem 272(49):30615–30618PubMedCrossRef

47.

Araki M, Nanri H, Ejima K, Murasato Y, Fujiwara T, Nakashima Y, Ikeda M (1999) Antioxidant function of the mitochondrial protein SP-22 in the cardiovascular system. J Biol Chem 274(4):2271–2278PubMedCrossRef

48.

Chae HZ, Kim HJ, Kang SW, Rhee SG (1999) Characterization of three isoforms of mammalian peroxiredoxin that reduce peroxides in the presence of thioredoxin. Diabetes Res Clin Pract 45(2–3):101–112PubMedCrossRef

49.

Radi R, Turrens JF, Chang LY, Bush KM, Crapo JD, Freeman BA (1991) Detection of catalase in rat heart mitochondria. J Biol Chem 266(32):22028–22034PubMed

50.

Knopp EA, Arndt TL, Eng KL, Caldwell M, LeBoeuf RC, Deeb SS, O’Brien KD (1999) Murine phospholipid hydroperoxide glutathione peroxidase: cDNA sequence, tissue expression, and mapping. Mamm Genome 10(6):601–605PubMedCrossRef

51.

Kalish RB, Nguyen DP, Vardhana S, Gupta M, Perni SC, Witkin SS (2005) A single nucleotide A>G polymorphism at position −670 in the Fas gene promoter: relationship to preterm premature rupture of fetal membranes in multifetal pregnancies. Am J Obstet Gynecol 192(1):208–212. doi:10.1016/j.ajog.2004.06.106 PubMedCrossRef

Title: Proteomic analysis of preterm premature rupture of membranes in placental tissue
Authors: Aimin Chang
Zhan Zhang
Linlin Zhang
Yan Gao
Ling Zhang
Liting Jia
Shihong Cui
Peng Wang
Publication date: 01-10-2013
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 4/2013
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-013-2837-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Proteomic analysis of preterm premature rupture of membranes in placental tissue

Abstract

Objection

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objection

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 4/2013

Message from the Editor-in-Chief

The change of LTB4 and 5-LO during pregnancy

The association of RANK gene C421T and C575T polymorphisms with bone mineral density in postmenopausal Turkish women

Combination of non-invasive and semi-invasive tests for diagnosis of minimal to mild endometriosis

Effects of gender roles, child wish motives, subjective well-being, and marital adjustment on infertility-related stress: a preliminary study with a Hungarian sample of involuntary childless men and women

Comparison of “sandwich chemo-radiotherapy” and six cycles of chemotherapy followed by adjuvant radiotherapy in patients with stage IIIC endometrial cancer: a single center experience