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01-12-2009 | Original Article

A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro

Authors: Dunja M. Baston-Büst, Alexandra P. Hess, Jens Hirchenhain, Jürgen Krücken, Frank Wunderlich, Jan S. Krüssel, Ulrike K. Friebe-Hoffmann

Published in: Archives of Gynecology and Obstetrics | Issue 6/2009

Abstract

Purpose

Based on the reported tocolytic action of the hormone relaxin (RLX) in rodents, locally produced in reproductive tissues and the corpus luteum in mammals, the present study aimed to evaluate the influence of RLX on contraction-mediating cyclooxygenases-1 and -2 (COX) and the contractile prostaglandin PGE₂ in human myometrial and decidual cells. Primary cultured cells were obtained from uteri and placentas of term and preterm women undergoing elective caesarean section.

Methods

In vitro culture of primary myometrial and decidual cells, immunocytochemistry, reverse transcription and real-time PCR, Western blot, ELISA.

Results

We demonstrate for the first time an activating effect of RLX for human COX-1 and COX-2 in primary myometrial and decidual cells in vitro.

Conclusions

These effects might potentially contribute to birth-associated induction of contractions in vivo.

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Title: A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro
Authors: Dunja M. Baston-Büst
Alexandra P. Hess
Jens Hirchenhain
Jürgen Krücken
Frank Wunderlich
Jan S. Krüssel
Ulrike K. Friebe-Hoffmann
Publication date: 01-12-2009
Publisher: Springer-Verlag
Published in: Archives of Gynecology and Obstetrics / Issue 6/2009
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-009-1046-8

2024 ASCO Annual Meeting

Springer Medicine

A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro

Abstract

Purpose

Methods

Results

Conclusions

2024 ASCO Annual Meeting

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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