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Archives of Gynecology and Obstetrics

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01-06-2009 | Original Article

Cytokine and immune cell levels in peritoneal fluid and peripheral blood of women with early- and late-staged endometriosis

Authors: H. Hassa, H. Mete Tanir, B. Tekin, S. D. Kirilmaz, F. Sahin Mutlu

Published in: Archives of Gynecology and Obstetrics | Issue 6/2009

Abstract

Objective

To investigate the level of cytokines and immune cells in the peripheral blood (PB) and peritoneal fluid (PF) of different stages of endometriosis.

Methods

A prospective study was conducted to include 97 women with (n 60) and without (n 37) histopathologically confirmed endometriosis. Based on rASRM classification, stage I/II and stage III/IV were categorized as early-and late-staged endometriosis. Prior to surgery, 10 ml of blood was withdrawn from antecubital vein and serum was obtained. Aliquots were made and stored at −70°C until assayed for cytokines. PF was aspirated from the pouch of Douglas. Peripheral and PF samples were analyzed by ELISA in terms of IL-2, IL-4, IL-10 and IFN-γ. Determinations of T helper, T suppressor, NK, and B cells were assessed by using cluster determinant-3 (CD-3), CD4, CD8, CD25, CD28, CD45, CD16, CD23 and antibodies against early T cell activation antigens such as CD45RA/CD45RO, CD-69 and late activation antigens such as HLA-DR. A multiparameter flow cytometry was applied to detect the cell activation antigen expression.

Results

In terms of cytokine levels in PB and PF’s of control group and early- and late-staged endometriosis cases, no significant difference was depicted in the cytokine levels (p > 0.05). Levels of immune cells did not differ between three groups (p > 0.05).

Conclusions

The result of this study did not show any significant difference in PB and PF cytokine and lymphocyte subgroups between normal and early- and late-staged endometriosis.

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Title: Cytokine and immune cell levels in peritoneal fluid and peripheral blood of women with early- and late-staged endometriosis
Authors: H. Hassa
H. Mete Tanir
B. Tekin
S. D. Kirilmaz
F. Sahin Mutlu
Publication date: 01-06-2009
Publisher: Springer-Verlag
Published in: Archives of Gynecology and Obstetrics / Issue 6/2009
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-008-0844-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Cytokine and immune cell levels in peritoneal fluid and peripheral blood of women with early- and late-staged endometriosis

Abstract

Objective

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Methods

Results

Conclusions

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