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01-12-2011 | Original Paper

CCL5, CXCL16, and CX3CL1 are associated with Henoch-Schonlein purpura

Authors: Tao Chen, Zai-pei Guo, Xiao-yan Jiao, Rui-zhen Jia, Yu-hong Zhang, Jing-yi Li, Xu-lei Huang, Hong-jie Liu, Ting Lai

Published in: Archives of Dermatological Research | Issue 10/2011

Abstract

Chemokines are involved in the pathogenesis of various vascular inflammations. However, information about chemokines in Henoch-Schonlein purpura (HSP) is limited. Herein, we investigated the serum CCL5, CXCL16, and CX3CL1 levels in HSP patients with controls and the ability of sera from HSP patients on chemokine production in human dermal microvascular endothelial cells. Enzyme-linked immunosorbent assay (ELISA) detected serum CCL5, CXCL16, and CX3CL1 levels in patients with HSP. Human dermal microvascular endothelial cell line (HMEC-1) was treated with sera from patients with HSP at different stages, patients with acute spontaneous urticaria, or controls. Serum levels of CCL5, CXCL16, and CX3CL1 were elevated in HSP patients at acute stage, which correlated with the severity of this disease. Sera from patients with active HSP markedly induced CCL5, CXCL16, and CX3CL1 production at both mRNA and protein levels. In addition, patients’ sera-stimulated HMEC-1 supernatants enhanced HL-60 or THP-1 cells migration. Furthermore, patients’ sera increased the phosphorylation of inhibitor of κB-α (IκBα) and phosphorylation of extracellular signal-regulated kinase (ERK)1/2 protein levels, upregulated the translocation of nuclear factor-κB (NF-κB) p65 to the nucleus. Taken together, we show firstly that CCL5, CXCL16, and CX3CL1 may be involved in the pathogenesis of HSP. Factors present in sera from patients with active HSP may act as an inducer of inflammatory response in HMEC-1 cells and contribute to chemokine production through NF-κB and ERK 1/2 pathways.

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Title: CCL5, CXCL16, and CX3CL1 are associated with Henoch-Schonlein purpura
Authors: Tao Chen
Zai-pei Guo
Xiao-yan Jiao
Rui-zhen Jia
Yu-hong Zhang
Jing-yi Li
Xu-lei Huang
Hong-jie Liu
Ting Lai
Publication date: 01-12-2011
Publisher: Springer-Verlag
Published in: Archives of Dermatological Research / Issue 10/2011
Print ISSN: 0340-3696
Electronic ISSN: 1432-069X
DOI: https://doi.org/10.1007/s00403-011-1150-z

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CCL5, CXCL16, and CX3CL1 are associated with Henoch-Schonlein purpura

Abstract

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