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01-01-2007 | Short Communication

In vivo levels of IL-4, IL-10, TGF-β1 and IFN-γ mRNA of the peripheral blood mononuclear cells in patients with alopecia areata in comparison to those in patients with atopic dermatitis

Authors: Kazumoto Katagiri, Shoko Arakawa, Yutaka Hatano

Published in: Archives of Dermatological Research | Issue 8/2007

Abstract

Alopecia areata (AA) has been considered to be supported by an aberrant expression of IFN-γ as a result of antigen dependent immune response. On the other hand, AA sometimes concurs with atopic diseases, although the mechanism of the concurrence is not clear. This study was designed to elucidate the immune status of AA and the similarity between AA and atopic dermatitis (AD) by analysis of in vivo levels of mRNA of Th1, Th2, and suppressive cytokines of peripheral blood mononuclear cells (PBMC). Using semiquantitative RT-PCR, the levels of cytokine mRNA were measured in freshly isolated PBMC of 47 patients with AA, 15 patients with AD, and 12 healthy controls (HC). The levels of IL-4, IFN-γ, and TGF-β1 mRNA were lower in patients with AA than those in HC. The levels of IL-10 mRNA in AA were comparable with those in HC. Decreased levels of IFN–γ and TGF-β1 were also shown in patients with AD. These results indicated a similarity (decreased levels of IFN-γ and TGF-β1) between AD and AA based on the cytokine profile. In addition, decreased levels of IL-4 mRNA in AA might also explain the experience that the severity of atopic disease coincident with AA is mild in the most of cases. Next, we compared the levels of these cytokine mRNA among the three subgroups of AA that were categorized based on the severity of the symptoms: mild, severe and totalis. Although there was no significant difference between any combinations of the subgroups, there was a tendency to increase the levels of IFN-γ mRNA and to decrease the levels of IL-4 mRNA according to the severity of alopecia. However, the levels of IFN-γ mRNA in any subgroups were less than those of HC. These results suggest that IFN-γ is therefore involved in the pathogenesis of AA, although the information from PBMC is limited. In conclusion, AA might be induced by an aberrant expression of IFN-γ in individuals whose PBMC produce low amounts of IFN-γ and TGF-β1. Further analysis is therefore required to investigate the phenotypes of the population in PBMC with or without reference to regulatory T cells.

Arakawa S, Hatano Y, Katagiri K (2004) Differential expression of mRNA for Th1 and Th2 cytokine-associated transcription factors and suppressors of cytokine signalling in peripheral blood mononuclear cells of patients with atopic dermatitis. Clin Exp Immunol 135:505–510PubMedCrossRef

Arca E, Musabak U, Akar A, Erbil AH, Tastan HB (2004) Interferon-gamma in alopecia areata. Eur J Dermatol 14:33–36PubMed

Bröcker EB, Echternacht-Happle K, Hamm H, Happle R (1987) Abnormal expression of class I and class II major histocompatibility antigens in alopecia areata: modulation by topical immunotherapy. J Invest Dermatol 87:564–568CrossRef

Deeths MJ, Endrizzi BT, Irvin ML, Steiner LP, Ericson ME, Hordinsky MK (2006) Phenotypic analysis of T-cells in extensive alopecia areata scalp suggests partial tolerance. J Invest Dermatol 126:366–373PubMedCrossRef

Hatano Y, Katagiri K, Ise T, Yamaguchi T, Itami S, Nawa Y, Takayasu S. (1999) Expression of Th1 and Th2 cytokine mRNAs in freshly isolated peripheral blood mononuclear cells of a patient with cutaneous paragonimiasis. J Dermatol Sci 19:144–147PubMedCrossRef

Hoffmann R, Wenzel E, Huth A, van der Steen P, Schäufele M, Henninger HP, Happle R (1994) Cytokine mRNA levels in Alopecia areata before and after treatment with the contact allergen diphenylcyclopropenone. J Invest Dermatol 103:530–533PubMedCrossRef

Jujo K, Renz H, Abe J, Gelfand EW, Leung DYM (1992) Decreased interferon gamma and increased interleukin-4 production in atopic dermatitis promotes IgE synthesis. J Allergy Clin Immunol 90:323–331PubMedCrossRef

Katagiri K, Itami S, Hatano Y, Takayasu S (1997) Increased levels of IL-13 mRNA, but not IL-4 mRNA, are found in vivo in peripheral blood mononuclear cells of patients with atopic dermatitis. Clin Exp Immunol 108:289–294PubMedCrossRef

Katagiri K, Itami S, Hatano Y, Yamaguchi T, Takayasu S (1997) In vivo expression of IL-4, IL-5, IL-13 and IFN-gamma mRNAs in peripheral blood mononuclear cells and effect of cyclosporin A in a patient with Kimura’s disease. Br J Dermatol 137:972–977PubMedCrossRef

10.

Mcdonagh AJG, Snowden JA, Stierle C, Elliott K, Messenger AG (1993) HLA and ICAM-1 expression in alopecia areata in vivo and in vitro: the role of cytokines. Br J Dermatol 129:250–256PubMedCrossRef

11.

Messenger AG, Bleehen SS (1985) Expression of HLA-DR by anagen hair follicles in alopecia areata. J Invest Dermatol 85:569–572PubMedCrossRef

12.

Rook A, Dawber R (1982) Diseases of the hair and scalp, 1st edn. Blackwell Scientific publications, Oxford, pp 298–302

13.

Taylor A, Verhagen J, Blaser K, Akdis M, Akdis CA (2006) Mechanisms of immune suppression by interleukin-10 and transforming growth factor-β: the role of T regulatory cells. Immunology 117:433–442PubMedCrossRef

14.

Yoshino T, Asada H, Ando Y, Fujii H, Yamaguchi Y, Yoshikawa K, Itami S (2001) Impaired responses of peripheral blood mononuclear cells to T-cell stimulants in alopecia areata patients with a poor response to topical immunothereapy. Br J Dermatol 145:415–421PubMedCrossRef

15.

Zöller M, McElwee KJ, Vitacolonna M, Hoffmann R (2004) The progressive state, in contrast to the stable or regressive state of alopecia areata, is reflected in peripheral blood mononuclear cells. Exp Dermatol 13:435–444PubMedCrossRef

16.

Zöller M, McElwee KJ, Vitacolonna M, Hoffmann R (2004) Apoptosis resistance in peripheral blood lymphocytes of alopecia areata patients. J Autoimmun 23:241–256PubMedCrossRef

Title: In vivo levels of IL-4, IL-10, TGF-β1 and IFN-γ mRNA of the peripheral blood mononuclear cells in patients with alopecia areata in comparison to those in patients with atopic dermatitis
Authors: Kazumoto Katagiri
Shoko Arakawa
Yutaka Hatano
Publication date: 01-01-2007
Publisher: Springer-Verlag
Published in: Archives of Dermatological Research / Issue 8/2007
Print ISSN: 0340-3696
Electronic ISSN: 1432-069X
DOI: https://doi.org/10.1007/s00403-006-0700-2

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In vivo levels of IL-4, IL-10, TGF-β1 and IFN-γ mRNA of the peripheral blood mononuclear cells in patients with alopecia areata in comparison to those in patients with atopic dermatitis

Abstract

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Abstract

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