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Archives of Orthopaedic and Trauma Surgery

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01-09-2012 | Basic Science

The optimal carrier for BMP-2: a comparison of collagen versus fibrin matrix

Authors: Sebastian Schützenberger, Arthur Schultz, Thomas Hausner, Rudolf Hopf, Gerald Zanoni, Tatjana Morton, Karl Kropik, Martijn van Griensven, Heinz Redl

Published in: Archives of Orthopaedic and Trauma Surgery | Issue 9/2012

Abstract

Introduction

The aim of our study was to investigate in vitro and in a new in vivo rat model for impaired bone healing whether a low dose BMP-2 preparation in fibrin would be equivalent or better than the combination of collagen and a high dose of BMP-2 which is currently in clinical use.

Materials and methods

In a 14 day period we compared the in vitro release kinetics of an absorbable collagen sponge (ACS) with 72 μg rhBMP-2 in the BMPC group and fibrin matrix with 10 μg rhBMP-2 in the BMPF group. In our in vivo experiment a critical sized osteotomy was performed in the rat femur, which was filled with a spacer, inhibiting bone formation for a period of 4 weeks. In a second operation this spacer was removed and the test item was applied into the defect. We compared the BMPF and BMPC groups with the ACS alone, FIBRIN alone and the EMPTY (4w/8w) control groups. 4 and 8 weeks after the second operation, specimens were analysed by X-ray and μCT imaging. Mechanically stable femurs were biomechanically evaluated.

Results

Cumulative BMP-2 release was five times higher in the BMPF group than in the BMPC group during the observation period. μCT analysis revealed that both the extent of bone union and the bone volume were significantly higher in the group with a lower dose of BMP-2 in fibrin matrix than in the groups without BMP-2 treatment. However there was no statistically significant difference between the BMPF and BMPC groups.

Conclusion

We conclude that fibrin matrix is an excellent carrier for BMP-2 and that it provides equivalent results with a sevenfold lower dose of BMP-2 compared with ACS.

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Title: The optimal carrier for BMP-2: a comparison of collagen versus fibrin matrix
Authors: Sebastian Schützenberger
Arthur Schultz
Thomas Hausner
Rudolf Hopf
Gerald Zanoni
Tatjana Morton
Karl Kropik
Martijn van Griensven
Heinz Redl
Publication date: 01-09-2012
Publisher: Springer-Verlag
Published in: Archives of Orthopaedic and Trauma Surgery / Issue 9/2012
Print ISSN: 0936-8051
Electronic ISSN: 1434-3916
DOI: https://doi.org/10.1007/s00402-012-1551-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The optimal carrier for BMP-2: a comparison of collagen versus fibrin matrix

Abstract

Introduction

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction

Materials and methods

Results

Conclusion

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