Top

Archives of Orthopaedic and Trauma Surgery

Published in:

01-01-2010 | Basic Science

In vitro testing of the osteoinductive potential of different bony allograft preparations

Authors: N. Bormann, A. Pruss, G. Schmidmaier, Britt Wildemann

Published in: Archives of Orthopaedic and Trauma Surgery | Issue 1/2010

Abstract

Introduction

Bony allografts are used frequently in the clinic for bone defect filling, however, less comparative data concerning their osteoinductive potential are available.

Aim

The purpose of the present study was the comparative analysis of different allograft preparations. From five donors, we investigated fresh-frozen cancellous bone (native), peracetic acid–ethanol sterilized (PES) cancellous bone, cortical bone and demineralised bone matrix (DBM). In addition, two commercially available DBM products from five different donors were analyzed: Allomatrix^® (Wright Medical Technology Inc.) and DBX putty^® (Synthes GmbH). For positive control and as a clinically used growth factor, BMP-2 was chosen.

Method

To investigate the osteoinductivity C2C12 cells were cultured with the different materials and the effect on cell proliferation and alkaline phosphatase activity were measured.

Result

Proliferation was significantly enhanced by the native cancellous bone, Allomatrix, and BMP-2 and decreased by the PES-processed cancellous bone. The osteogenic differentiation was significantly enhanced by BMP-2 and the two commercial DBM products and decreased by PES-sterilized cancellous bone. All tested materials revealed a high donor-dependent variability. This is the first comparative study on the osteoinductivity of bony allografts frequently used in clinic.

Alt V, Heissel A (2006) Economic considerations for the use of recombinant human bone morphogenetic protein-2 in open tibial fractures in Europe: the German model. Curr Med Res Opin 22(Suppl 1):S19–S22

Bonewald LF (2002) Transforming growth factor-β. In: Bilezikan JP, Raisz LG, Rodan GA (eds) Principles of bone biology. Academic Press, San Diego, pp 903–918

DeLong WG Jr, Einhorn TA, Koval K, McKee M, Smith W, Sanders R et al (2007) Bone grafts and bone graft substitutes in orthopaedic trauma surgery. A critical analysis. J Bone Jt Surg Am 89:649–658CrossRef

Dinopoulos HT, Giannoudis PV (2006) Safety and efficacy of use of demineralised bone matrix in orthopaedic and trauma surgery. Expert Opin Drug Saf 5:847–866

Drosos GI, Kazakos KI, Kouzoumpasis P, Verettas DA (2007) Safety and efficacy of commercially available demineralised bone matrix preparations: a critical review of clinical studies. Injury 38(Suppl 4):S13–S21

Eufinger H, Leppanen H (2000) Iliac crest donor site morbidity following open and closed methods of bone harvest for alveolar cleft osteoplasty. J Craniomaxillofac Surg 28:31–38PubMed

Finkemeier CG (2002) Bone-grafting and bone-graft substitutes. J Bone Jt Surg Am 84-A:454–464

Glowacki J (2005) A review of osteoinductive testing methods and sterilization processes for demineralized bone. Cell Tissue Bank 6:3–12CrossRefPubMed

Govender S, Csimma C, Genant HK, Valentin-Opran A, Amit Y, Arbel R et al (2002) Recombinant human bone morphogenetic protein-2 for treatment of open tibial fractures: a prospective, controlled, randomized study of four hundred and fifty patients. J Bone Jt Surg Am 84-A:2123–2134

10.

Greenwald AS, Boden SD, Goldberg VM, Khan Y, Laurencin CT, Rosier RN (2001) Bone-graft substitutes: facts, fictions, and applications. J Bone Jt Surg Am 83-A(Suppl 2) Pt 2:98–103

11.

Han B, Tang B, Nimni ME (2003) Quantitative and sensitive in vitro assay for osteoinductive activity of demineralized bone matrix. J Orthop Res 21:648–654CrossRefPubMed

12.

Han B, Yang Z, Nimni M (2005) Effects of moisture and temperature on the osteoinductivity of demineralized bone matrix. J Orthop Res 23:855–861CrossRefPubMed

13.

Hill PA (1998) Bone remodelling. Br J Orthod 25:101–107CrossRefPubMed

14.

Jortikka L, Laitinen M, Wiklund J, Lindholm TS, Marttinen A (1998) Use of myoblasts in assaying the osteoinductivity of bone morphogenetic proteins. Life Sci 62:2359–2368CrossRefPubMed

15.

Katagiri T, Yamaguchi A, Komaki M, Abe E, Takahashi N, Ikeda T et al (1994) Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage. J Cell Biol 127:1755–1766CrossRefPubMed

16.

Levander G (1945) Tissue induction: nature 155:148–149

17.

Pinholt EM, Solheim E (1998) Osteoinductive potential of demineralized rat bone increases with increasing donor age from birth to adulthood. J Craniofac Surg 9:142–146CrossRefPubMed

18.

Pruss A, Baumann B, Seibold M, Kao M, Tintelnot K, Von Versen R et al (2001) Validation of the sterilization procedure of allogeneic avital bone transplants using peracetic acid–ethanol. Biologicals 29:59–66CrossRefPubMed

19.

Schmidmaier G, Wildemann B, Stemberger A, Haas NP, Raschke M (2001) Biodegradable poly(d,l-lactide) coating of implants for continuous release of growth factors. J Biomed Mater Res Appl Biomat 58:449–455CrossRef

20.

Schwartz Z, Somers A, Mellonig JT, Carnes DL Jr, Dean DD, Cochran DL et al (1998) Ability of commercial demineralized freeze-dried bone allograft to induce new bone formation is dependent on donor age but not gender. J Periodontol 69:470–478PubMed

21.

Sen MK, Miclau T (2007) Autologous iliac crest bone graft: should it still be the gold standard for treating nonunions? Injury 38(Suppl 1):S75–S80CrossRefPubMed

22.

Shigeyama Y, D’Errico JA, Stone R, Somerman MJ (1995) Commercially-prepared allograft material has biological activity in vitro. J Periodontol 66:478–487PubMed

23.

Silber JS, Anderson DG, Daffner SD, Brislin BT, Leland JM, Hilibrand AS et al (2003) Donor site morbidity after anterior iliac crest bone harvest for single-level anterior cervical discectomy and fusion. Spine 28:134–139CrossRefPubMed

24.

Spemann H, Mangold H (2001) Induction of embryonic primordia by implantation of organizers from a different species. 1923. Int J Dev Biol 45:13–38PubMed

25.

Traianedes K, Russell JL, Edwards JT, Stubbs HA, Shanahan IR, Knaack D (2004) Donor age and gender effects on osteoinductivity of demineralized bone matrix. J Biomed Mater Res B Appl Biomater 70:21–29CrossRefPubMed

26.

Urist MR (1953) Physiologic basis of bone-graft surgery, with special reference to the theory of induction. Clin Orthop 1:207–216PubMed

27.

Urist MR, Strates BS (1971) Bone morphogenetic protein. J Dent Res. 50:1392–1406PubMed

28.

Wildemann B, Kadow-Romacker A, Pruss A, Haas NP, Schmidmaier G (2007) Quantification of growth factors in allogenic bone grafts extracted with three different methods. Cell Tissue Bank 8:107–114CrossRefPubMed

29.

Yamaguchi A, Ishizuya T, Kintou N, Wada Y, Katagiri T, Wozney JM et al (1996) Effects of BMP-2, BMP-4, and BMP-6 on osteoblastic differentiation of bone marrow-derived stromal cell lines, ST2 and MC3T3–G2/PA6. Biochem Biophys Res Commun 220:366–371CrossRefPubMed

30.

Zhang M, Powers RM Jr, Wolfinbarger L Jr (1997) Effect(s) of the demineralization process on the osteoinductivity of demineralized bone matrix. J Periodontol 68:1085–1092PubMed

Title: In vitro testing of the osteoinductive potential of different bony allograft preparations
Authors: N. Bormann
A. Pruss
G. Schmidmaier
Britt Wildemann
Publication date: 01-01-2010
Publisher: Springer-Verlag
Published in: Archives of Orthopaedic and Trauma Surgery / Issue 1/2010
Print ISSN: 0936-8051
Electronic ISSN: 1434-3916
DOI: https://doi.org/10.1007/s00402-009-0908-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

In vitro testing of the osteoinductive potential of different bony allograft preparations

Abstract

Introduction

Aim

Method

Result

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction

Aim

Method

Result

Please log in to get access to this content

Other articles of this Issue 1/2010

Digastric trochanteric flip osteotomy and surgical dislocation of hip in the management of acetabular fractures

Injury of the right and left inferior epigastric artery during the implantation of a stand-alone ALIF cage through a left retroperitoneal approach: a case report

Complete polyethylene wear-through and secondary breakage of the expansion cup in a ceramic-polyethylene total hip arthroplasty

Quadriceps force in relation of intrinsic anteroposterior stability of TKA design

An unusual case of chondrolysis of the hip following excision of a torn acetabular labrum

Biodegradable microspherical implants containing teicoplanin for the treatment of methicillin-resistant Staphylococcus aureus osteomyelitis