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Open Access 01-09-2014 | Original Paper

Brain pericytes acquire a microglial phenotype after stroke

Authors: Ilknur Özen, Tomas Deierborg, Kenichi Miharada, Thomas Padel, Elisabet Englund, Guillem Genové, Gesine Paul

Published in: Acta Neuropathologica | Issue 3/2014

Abstract

Pericytes are located on the abluminal side of endothelial cells lining the microvasculature in all organs. They have been identified as multipotent progenitor cells in several tissues of the body including the human brain. New evidence suggests that pericytes contribute to tissue repair, but their role in the injured brain is largely unknown. Here, we investigate the role of pericytes in ischemic stroke. Using a pericyte-reporter mouse model, we provide unique evidence that regulator of G-protein signaling 5 expressing cells are activated pericytes that leave the blood vessel wall, proliferate and give rise to microglial cells after ischemic brain injury. Consistently, we show that activated pericytes express microglial markers in human stroke brain tissue. We demonstrate that human brain-derived pericytes adopt a microglial phenotype and upregulate mRNA specific for activated microglial cells under hypoxic conditions in vitro. Our study indicates that the vasculature is a novel source of inflammatory cells with a microglial phenotype in brain ischemia and hence identifies pericytes as an important new target for the development of future stroke therapies.

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Title: Brain pericytes acquire a microglial phenotype after stroke
Authors: Ilknur Özen
Tomas Deierborg
Kenichi Miharada
Thomas Padel
Elisabet Englund
Guillem Genové
Gesine Paul
Publication date: 01-09-2014
Publisher: Springer Berlin Heidelberg
Published in: Acta Neuropathologica / Issue 3/2014
Print ISSN: 0001-6322
Electronic ISSN: 1432-0533
DOI: https://doi.org/10.1007/s00401-014-1295-x

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Brain pericytes acquire a microglial phenotype after stroke

Abstract

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